B. AP SOW_f.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

STATEMENT OF WORK (SERVICES)

(SOW)

GENERAL INFORMATION

Title of Project:

Custom Knockin Cell lines

Statement of Need and Purpose:

The purpose of this procurement is the acquisition of custom knockin cell lines or the Functional Genomics Lab. The National Center for Advancing Translational Sciences (NCATS) requires services for custom knockin cell lines from cells that NCATS will provide to the Contractor.

Background Information and Objective:

The National Institutes of Health (NIH) has established an RNAi facility to provide intramural researchers with access to the equipment and expertise necessary to conduct genome-wide RNAi screens in mammalian cells. This facility works as a collaborative resource for doing RNAi assay development, screening, and initial follow up. Our facility works with investigators to develop and optimize their assays for screening, conduct the screens and related informatics analyses, then performs follow-up screens on promising leads. Further work is done with the PI to do the downstream biology required to get the quality publications and the kind of groundbreaking results needed. This request is required for this facility to develop a flexible functional genomics platform that offers complementary and orthogonal CRISPR screens to NIH intramural investigators. Cellecta synthesizes state of the art clonal cell lines that follow the RNAi workflow closely. This item is needed to facilitate our RNAi screeningThis program is to provide intramural researchers with access to the equipment and expertise necessary to conduct large scale functional genomics screens. The cell lines being requested will facilitate this program by completing an important mission related project at NCATS. The cell lines that are specifically being requested from this Contractor would be in the programs best interest due to the high possibility that obtaining colonal lines from another service would skew the existing data and would then require re-optimization of established workflow.

Period of Performance:

One-Time delivery 3 months after receipt of order

SCOPE OF WORK

General Requirements:

Independently and not as an agent of the Government, the Contractor shall furnish all the necessary services, qualified personnel, material, equipment, and facilities, not otherwise provided by the Government as needed to perform the Statement of Work below:

Specific Requirements:

Cellecta will generate tet-inducible dCas9-KRAB clonal lines from parental NPC24, NPC25, NPC57 cells, constitutive dCas9-KRAB clonal lines from parental PEO1_OlaR, PEO1_OlaJR, PEO4 cells, constitutive wtCas9 clonal line from parental RAW264.7-Trex1 cells. All parental cells will be provided by the customer.

LEVEL OF EFFORT:

Deliverables (2 -3 months: ~7 for cell pools and ~7 for clonal selection)

GOVERNMENT RESPONSIBILITIES

NCATS will provide Cellecta with the following cell lines:NPC24, NPC25, NPC57 and PEO1_OlaR, PEO1_OlaJR, PEO4 cells and RAW264.7-Trex1 parental cells

DELIVERY OR DELIVERABLES

Deliverables (2 -3 months: ~7 for cell pools and ~7 for clonal selection)

REPORTING REQUIREMENTS

Contractor shall provide data upon completion of the custom knockin cell lines

OTHER CONSIDERATIONS

Travel:

Key Personnel:

Information System Security Plan:

Data Rights:

The Government assumes the ownership of all data generated in the course of this project. The Contractor agrees that any data provided to carry out the project are confidential and cannot be disclosed to a third party without the consent of the Customer.

Section 508—Electronic and Information Technology Standards:

Publications and Publicity:

Confidentiality of Information:

ATTACHMENTS

Instructions: Include the following attachments with all SOWs

1. Independent Government’s Cost Estimate

2. Evaluation / Selection Criteria