Attachment No 3 Salient Characteristics Official Release.pdf

ATTACHMENT NO. 3

SALIENT CHARACTERISTIC

Item: Inverted Microscope w/Fluorescence, camera and imaging software

Vendor: Nikon

Model: Ti2E

Performance Features:

1. Inverted Microscope with fluorescence, brightfield, camera and imaging software with workstation.

2. The inverted microscopy system with diverse imaging and acquisition and analysis methods should include multidimensional time-lapse imaging to acquire temporal, spatial and spectra information of fast, dynamic live cell processes.

3. The Microscope should have a motorized stand and motorized components. Objective lenses should include a 10x, 20x, 40x and 60x fluorescent and apochromatic Lens.

4. Filter sets for fluorescent viewing of DAPI, FITC/Cy2, and Texas Red.

5. Cameras need to be able collect monochromatic and color images.

6. Microscope needs to have a workstation with software for acquisition and analysis of images. Workstation needs sufficient processing and RAM to easily run advanced software acquisition and analysis of images.

7. As a minimum the system should have:

a. a 4 Core, 3.6 GHz processor (4 cores)

b. 32GB DDR4 2666 Mhz RAM

c. P4000 8GB GDDR5X GPU

d. 512GBTurbo M.2 OS drive.

e. Minimum storage must be at least 2 TB storage

f. Must have keyboard and optical mouse.

g. Must have Windows 10 64-bit Professional operating system.

h. Acquisition software must include capture capabilities of time laps, z-stack, multi stage-position, multichannel fluorescence, AVI acquisition and intensity correction.

i. Software for displaying and processing images must include annotation, 3D imaging, volume rendering & movie making, and montage viewing.

j. Software must be expandable to meet needs of future research including TIRF, FRET and photobleaching/photoactivation to study the dynamics and interactions of fluorescent protein molecules in living cells and tissues.

System Features System Must:

1. Be an inverted microscopy system supporting Stable Time-lapse Imaging with Automatic

Focus Correction System

2. Have improved Performance in Broad Wavelength Range

3. Maintain Focus at Greater Depths, Compatible with Plastic Cell Culture Dishes and Cell

Culture Well Plates

4. Have High-speed Motorized Control and Acquisition

5. Have High-quality Phase Contrast Images using High NA Lenses

6. Be capable of Advanced Integration with Peripherals via NIS- Elements

7. Have Imaging Software with Ti-LAPP Modular Illumination System, DMD Module, H-

TIRF Module, Flexible Module Combination, Two tiered Configuration Capability, and

Multiport Design with a Maximum of Five Imaging Ports.

Physical Specifications:

System Dimensions and Weight: Width 50 in. x Depth 23 in. x Height 30 in.

Electrical Requirements: 120-240 VAC, 15A