Attachment A_SOW_Genome Sequencing.pdf
PDF 119 KB Posted
- Attached to
- Genome Sequencing Federal contract opportunity
- Solicitation number
- 1232SA25Q0273
About this file
This Statement of Work (SOW) details a genome sequencing requirement for the United States Department of Agriculture (USDA) Agricultural Research Service (ARS) Animal Parasitic Diseases Laboratory in Beltsville, MD. The laboratory seeks a single cell genomic service to support scientific research on cellular dynamics of host-parasite interactions in goats, sheep, and rodents, with a focus on understanding molecular changes in complex gut tissue cell populations.
The SOW specifies technical requirements including tissue dissociation, single-cell library preparation using 10X Genomics Chromium GEM-X Single Cell Gene Expression kits, and next-generation sequencing using an Illumina NovoSeq X sequencer. Key technical specifications include processing 31 tissue samples in two batches, achieving 10,000-20,000 cells per sample, maintaining >75% cell type recovery efficiency, detecting rare cell types at 0.01-0.05% frequency, and generating 30,000-50,000 read pairs per cell. The contract duration is 12 months with one optional 1-year extension, requiring Illumina standard format data output with full digital gene expression signature profile analysis.
View the file
Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Attachment C_Wage Rates.pdf | ||
| Attachment B_Solicitation Clauses and Provisions_Genome Sequencing.pdf | ||
| Combined Synopsis Solicitation_Genome Sequencing.pdf |
On GovTribe
Work with this file on GovTribe
- Download the original file
- Contacts named in this file
- Similar government files
- Ask GovTribe AI about this file
Text version
Genome Sequencing
United States Department of Agriculture (USDA)
Animal Parasitic Diseases Laboratory
1.0 Introduction
1.1 The Agricultural Research Service (ARS) is the U.S. Department of Agriculture's chief scientific in-house research agency with the mission to deliver scientific solutions to national and global agricultural challenges. The ARS has been tasked to find solutions to agricultural problems that affect
Americans every day from field to table. The ARS delivers cutting-edge, scientific tools and innovative solutions for American farmers, producers, industry, and communities to support the nourishment and well-being of all people; sustain our nation’s agroecosystems and natural resources; and ensure the economic competitiveness and excellence of our agriculture.
1.2 The USDA, ARS Animal Parasitic Diseases Laboratory (APDL), located in Beltsville, MD, has a requirement for Genome Sequencing.
2.0 Objectives
2.1 This requirement is for Genome Sequencing.
2.2 The USDA APDL conducts basic and applied scientific research to understand cellular dynamics of host-parasite interactions in goats, sheep, and rodents in response to antiparasitic drug treatments. To accomplish project milestones, APDL needs ready access to advanced sequencing technologies to characterize molecular changes in individual cells in a complex gut tissue consisting of many cell types, including Tuft cells, goblets, and various immune cells (e.g., T cells, macrophages, dendritic cells, and neutrophils).
3.0 Requirements
3.1 The single cell genomic service must include the following three interdependent processes.
3.1.1 Tissue dissociation using enzymatic and mechanical approaches to disintegrate tissues into live, intact, single cell suspensions or homogenates while preserving cellular integrity.
3.1.2 Single-cell library preparation using 10X Genomics Chromium GEM-X Single Cell Gene
Expression 3' v4 kits and related specialized instruments.
3.1.3 Next-generation sequencing using an Illumina NovoSeq X sequencer and data delivery and analysis.
4.0 Additional Requirements
4.1 Required throughput: 10,000 to 20,000 cells per sample for 31 fresh or frozen tissue samples submitted in two batches.
4.2 Sample recovery: >75% cell type recovery efficiency.
4.3 Sensitivity: Must be able to detect rare cell types at a frequency of 0.01% to 0.05% of total cell populations as well as fragile cells, such as regulatory T cells (Treg), and cells that are difficult to isolate.
4.4 Sequencing depth: Must be 30,000 to 50,000 read pairs per cell, or 80 to 100 Gb sequences per sample.
4.5 Data Output: Illumina standard formats, must provide digital gene expression signature profiles with full data analysis.
5.0 Deliverable/Schedule
5.1 12 months with one 1-year optional period.
File details come from the government source that posted it. Updated .