Attachment 2_SOW Cotton DNA Sequencing.pdf
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- Attached to
- Cotton DNA Sequencing Services Federal contract opportunity
- Solicitation number
- 1232SA26Q0389
About this file
This is a Statement of Work for DNA sequencing services issued by the USDA Agricultural Research Service (ARS) Cotton Fiber Bioscience & Utilization Research Unit in New Orleans, Louisiana. The contract requires next-generation sequencing of 550 cotton leaf DNA samples to support research objectives under the NP301 Plant Genetic Resources, Genomics, and Genetic Improvement program. The work aims to understand genome structure in an advanced cotton population and identify sequence variants between progeny plants to facilitate development of improved cotton varieties.
The contractor must perform quality assessment and bio-analysis of extracted DNA from young cotton leaf tissues, conduct PCR-free library preparation for each sample, assess library quality, and complete next-generation sequencing using an Illumina NovaSeq X 10B platform (300 cycles shared flowcell) or similar platform with 2 x 150 paired-end reads and a guaranteed output of 50 Gigabases per sample. Low-quality samples must be replaced prior to library preparation to ensure only high-quality samples are sequenced. All 550 samples must be processed together in a single batch to maintain consistency and optimize data quality. The contractor must deliver raw sequence reads in fasta or fastq format for all samples on a hard disk shipped to the customer, with a guaranteed turnaround time of 45 business days from sample receipt. The contractor must demonstrate specialized experience with all aspects of DNA sequencing operations.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Attachment 1 - Solicitation Terms and Conditions_Cotton DNA Sequencing.pdf | ||
| Attachment 3_Wage Rates.pdf |
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STATEMENT OF WORK
DNA Sequencing Service of Cotton Leaf Tissues
Background USDA, Agricultural Research Service (ARS), Cotton Fiber Bioscience & Utilization Research Unit in New Orleans, LA has a requirement for the next-generation sequencing of 550 cotton leaf DNA samples. The data generated will help us to understand the genome structure of an advanced population and identify sequence variants between the progeny plants. This work will help to breed better cotton varieties and advance our research objectives under NP301 Plant Genetic Resources, Genomics, and Genetic Improvement.
Statement of Work Sequencing 550 DNAs extracted from cotton leaf tissues. This will include DNA quality assessment, library preparation, library quality assessment, and sequencing.
Specifically, DNA extracted from young cotton leaf tissues will be evaluated for quality through bio-analysis. High-quality DNA will be used to generate sequencing libraries. A PCR-free preparation method must be used to construct a specialized library using each of the provided DNA samples.
Additionally, any samples that are deemed of a lower quality must be replaced prior to moving on to library preparation, guaranteeing only high-quality samples will be sequenced.
Next Generation Sequencing must be completed using the Illumina NovaSeq X 10B (300 cycles shared flowcell) or similar platform with a read length of 2 x 150 (Paired End), with a guaranteed sequencing output of 50 Gigabases (Gb) per sample.
All samples must be run together in a single batch to maintain consistency and ensure optimal data quality.
The guaranteed turnaround time from the date of receiving DNA samples must be 45 business days; this is a minimum requirement. The company must have specialized experience with all aspects of DNA sequencing.
Schedule of Line Items Line Item – quality assessment, PCR free library preparation, and sequencing of 550 cotton DNA samples.
Deliverable will include raw sequence reads in fasta or fastq format for each of 550 cotton DNA samples in a hard disk and shipped to the customer.
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