Attachment 1 Specs and Salient Characteristics.docx
DOCX document 35 KB Posted
- Attached to
- Nanostring nCounter Sprint Profiler Federal contract opportunity
- Solicitation number
- 12305B22Q0109
View the file
Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| 12305B22Q0109.pdf |
On GovTribe
Work with this file on GovTribe
- Download the original file
- Contacts named in this file
- Similar government files
- Ask GovTribe AI about this file
Text version
PRODUCT SPECIFICATION
TITLE: nanoString nCounter SPRINT Profiler nanoString Relevant Characteristics nanoString is a novel technology for spatial profiling biology. This platform offers a unique capability to multiplexed (up to 800 genes) spatial profiling of RNA, DNA, and cell lysate from diverse sample types, including challenging Formalin-Fixed, Paraffin-Embedded (FFPE) tissue. Importantly, this technology is optimized to obtained same quality of data regardless of whether the sample processing is from freshly collected tissue or from previously fixed samples.
The nCounter SPRINT Profiler employs a novel digital barcode technology. These barcodes hybridize with target molecules, and unique digital barcodes are then directly counted using a single-molecule imaging to yield precise and sensitive quantification. Up to 800 targets can be measured simultaneously in a single sample, and up to 12 samples can be assayed in a single run.
The following nCounter® applications are supported:
-Vantage™ DNA, RNA (fusion) and Protein panels -Gene expression (mRNA) -MicroRNA (miRNA) -DNA copy number variation (CNV) -Long non-coding RNA (lncRNA) -DNA enriched by chromatin-immunoprecipitation (ChIP-String).
Key Features and Benefits
| Feature |
| Benefit |
Pre-designed Assays
No time wasted with assay optimization.
| Automation | |
| Accuracy and reproducibility for greater confidence in results. |
Enzyme-free Chemistry
Success using degraded samples (e.g., FFPE tissues).
| Digital Counting | |
| Quantifiable data output for easier, more reliable analysis. |
Single-tube Assay of up to 800 Targets
Better view of whole pathways or gene signatures and the ability to include additional housekeeping genes without extra effort.
Crude Cell Lysate Sample Input
Eliminate cost and time associated with nucleic acid extraction.
Minimal Hands-on Time
Fewer human resources required.
Platform Performance Specifications
| Description |
| Specification |
| Level of Target Multiplexing |
| Up to 800 targets |
| Recommended Amount of Starting Material |
| RNA: 25 ng if > 400 target plex 50 ng if < 400 target plex |
DNA: 150 ng RNA:Protein: 200–2,500 cell equivalent lysates
| Sample Types Supported |
| Total RNA, cell lysates in GITC, total RNA derived from FFPE tissues, and PAXgene™-lysed whole blood |
| Reaction Volume |
| Up to 35 μL |
| Limit of Detection |
| 15 zeptomole spike-in control |
| Fold Change Sensitivity |
| > 1.5-fold (if > 5 copies per cell) > 2-fold (if > 1 copy per cell) |
| Spike Correlation |
| R2 ≥ 0.95 |
| Linear Dynamic Range |
| 6 x 105 total counts |
| Throughput |
| 12 lanes per 6 hours |
Hardware Specification
| Description |
| Specification |
| Operating Temperature |
| 18–28°C |
| Humidity |
| 30-80% |
| Pollution Degree |
| 2 |
| Power Source |
| 100–240 VAC, 50–60 Hz |
| Dimensions |
| 91 x 76 x 53 cm |
| Weight |
| 81.65 |
File details come from the government source that posted it. Updated .