Attachment 1 Specifications.pdf
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- Attached to
- Two (2) qPCR-Real Time Systems (Parlier, CA) Federal contract opportunity
- Solicitation number
- 12905B23Q0157
About this file
This document outlines specifications for two quantitative polymerase chain reaction (qPCR) systems. The Agricultural Research Service of the Department of Agriculture seeks to procure one two-target qPCR system equivalent to Bio-Rad's CFX Duet Real-Time PCR System, with the ability to detect two DNA targets per well in a multiplex assay. Specifications include excitation/emission wavelengths of 450-580nm, a temperature range of 4-100°C, and the ability to detect one copy of target sequence. Additionally, one five-target qPCR system is required equivalent to Bio-Rad's CFX Opus 96 Real-Time PCR System, with standalone capabilities to detect up to five DNA targets per well in a multiplex assay. Specifications include excitation/emission wavelengths of 450-684nm and 515-730nm respectively, and the same temperature and detection requirements. Both systems must include appropriate software and reagents.
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| RFQ_12905B23Q0157 Terms and Conditions.pdf |
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12905B23Q0157 Parlier, CA
Attachment 1 – Specifications
USDA ARS SJVASC-CDPG requires two qPCR-Real Time systems (RT-qPCR), one of which can detect two DNA targets in a multiplex qPCR assay and the other capable of detecting up to five DNA targets in a multiplex qPCR assay.
Performance Specifications and Salient Characteristics (Brand Name or Equal):
Two Target RT-qPCR System, equal to BioRad CFX Duet Real-Time PCR System
This instrument must be capable of processing 96 reactions, with multiplex processing of two target genes/well simultaneously. The Real-Time System needs to use Fluorescence Resonance Energy Transfer (FRET) optics to detect target DNA amplicons which increases accuracy and the reliability of data.
SPECIFICATIONS of Two Target qPCR Real-Time System:
Reliable 96-well qPCR system with quick setup, easy installation, and factory-calibrated optics Multiplex analysis – up 2 DNA targets/well (plus FRET) simultaneously without the need for reference/normalizing dye Excitation/emission wavelength range covering 450-580nm Optical detection – excitation (3 filtered LEDs), detection (3 filtered photodiodes) Average ramp rate of at least 3.3⁰C/sec Thermocycler lid temperature range of 30-110⁰C Temperature range of 4-100⁰C Sensitivity: Capable of detecting one copy of target sequence in human genomic DNA Accuracy of +/- 0.2⁰C or better at 90⁰C Ability to process reaction volumes of 1-50µL Dynamic range of ten orders of magnitude Software (CFX Maestro v2.3 or equivalent) for Windows and Mac, with license Uniform optical system free of regular calibration requirements Audit and e-signature compatibility One year warranty iScript Reverse Transcription Supermix, SsoAdvanced Universal SYBR Green Supermix, and iTaq
Universal SYBR Green Supermix included
Five Target RT-qPCR System, equal to Bio-Rad CFX Opus 96 Real-Time PCR System
This instrument must have standalone features, and be capable of processing 96 reactions, with multiplex processing of up to five DNA targets (plus FRET) simultaneously.
Specifications of 5 Target qPCR Real-Time System:
Standalone capabilities (functional without hooking up to a computer) to be used for experiments in gene expression (without requiring ROX), end-point analysis for sequence
12905B23Q0157 Parlier, CA detection, and protein thermal shift assays Touchscreen interface Simultaneous multiplex processing of up to 5 target DNA sequences FRET Detection (Fluorescence Resonance Energy Transfer) of target DNA Excitation/Detection: 6 filtered LEDs/ 6 filtered photodiodes Excitation wavelength range covering 450-684nm Emission wavelength range covering 515-730nm Average ramp rate of at least 3.3⁰C/sec Thermocycler lid temperature 30-110⁰C Temperature range 4-100⁰C Accuracy of +/- 0.2⁰C or better at 90⁰C Sensitivity: capable of detecting one copy of target sequence in human genomic DNA Dynamic range of 10 orders of magnitude Ability to process reaction volumes of 1-50µL Uniform optical system free of regular calibration requirements Software (CFX Maestro v2.3) for Windows and Mac, with license included Audit and e-signature compatibility iScript Reverse Transcription Supermix (or equivalent), SsoAdvanced Universal SYBR Green
Supermix, and iTaq Universal SYBR Green Supermix One year warranty
File details come from the government source that posted it. Updated .