Attachment 1 Specifications and Salient Characteristics.pdf

REQUEST FOR QUOTATION (RFQ)

RFQ # 12905B22Q0231 Real-Time PCR Instrument (Salinas, CA)

Attachment 1

Specifications and Salient Characteristics (Brand Name or Equal)

One Real-Time PCR Instrument

The following thermal details are required to execute experimental PCR protocols that were developed from 2019 to 2022 and optimized specifically for use on a Real-Time PCR Instrument that has the following specifications: Maximum ramp rate, 5 °C/sec; Average ramp rate, 3.3 °C/sec; Peltier heating and cooling method; Lid = 30–110 °C; Temperature Range = 4-100 °C;

Accuracy = ±0.2 °C of programmed target at 90°C; Uniformity = ±0.3 °C well-to-well after 10 sec of arrival at target; Operational range = 30–100 °C; Programmable span = 1-24 °C.

The following optical/detection details are required to execute experimental PCR protocols that were developed from 2019 to 2022: 6 filtered LEDs with excitation ranges between 450–684 nm and 6 filtered photodiodes for detection between 450–650 nm to allow for multiplexing and detection of up to 6 genetic targets using established PCR protocols. The sensitivity of the machine must be equivalent to 1 copy of target sequence from human genomic DNA with a dynamic range of 10 orders of magnitude.

The following connectivity and dimension details are needed to acquire and transfer the data in a limited space within the laboratory: Communication interface = USB 2.0; Electrical approvals = IEC, CE; Dimensions (W x D x H), cm/in = 33x56x36 / 13x22x14; Weight, kg/lb = 22/48;

Touch Screen Adjustable from 15°–22°.

The following software and hardware is needed to set up experiments and analyze the data, and is necessary for compatibility with experiments run previously. Software that can support real-time PCR plate setup, data collection, statistics, and graphing of results. A Windows PC Wi-Fi Adapter to enable Wi-Fi connection to the Real-Time PCR Instrument in North America. 1 Computer PC for software and database to communicate with and store data generated by the system. Computer monitor for viewing chromatograms and actions performed on the PC. It is necessary to continue expansion of our program using the same technology to allow comparison of previously generated and newly generated data.

REQUEST FOR QUOTATION (RFQ)

RFQ # 12905B22Q0231 Real-Time PCR Instrument (Salinas, CA)

ITEM DESCRIPTION QTY

CFX Opus 96 Real-Time PCR Instrument 96-well, 5-color plus FRET, networkconnected real-time PCR detection system, includes CFX Opus 96 base unit, cables 1

CFX Maestro Software 2.3 for Windows PC 1 license, CFX Maestro 2.3, software for real-time PCR plate setup, data collection, statistics, and graphing of results; for Windows PCs 2

CFX Opus Wi-Fi Adapter, North America & Others Wi-Fi antenna enables Wi-Fi connection for CFX Opus in North America and other specific countries. 1

CFX Maestro Software, Mac Edition Mac edition of CFX Maestro Software for real-time PCR plate setup, data analysis, statistics, and graphing of results 10

Computer PC for ChromLab Software and database, communicates with and stores data generated by the NGC System 1

Monitor, 22" for use with 788-4015, for viewing chromatograms and actions performed on the PC 1