Attachment 1 - Purchase Description Light Sheet Microscope.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Multispectral Light Sheet Microscope System

1. Statement of Need and Purpose: The National Institute of Neurological Disorders and

Stroke (NINDS) Flow and Imaging Cytometry Core Facility (FICCF) provides routine use of conventional flow cytometry, imaging cytometry, fluorescence-activated cell sorting (FACS) and in situ multispectral microscope imaging technology and application techniques to assay a multitude of biological properties at the molecular, subcellular, cellular and tissue levels, as specified by NINDS and other NIH intramural investigators.

To this end, the NINDS FICCF owns flow cytometry and imaging equipment which requires periodic upgrades, replacements and/or addition of new state-of-the-art equipment featuring new bioassay technologies and techniques to ensure full operational performance to optimally support continuoulsly evolving research requirements performed at the Facility. FICCF currently needs to purchase a new state-of-the-art multispectral light sheet imaging system in order to continue to innovate and provide optimal services to NINDS and other Institutes at NIH, which collaborate with

NINDS.

2. Background Information and Objective: NINDS FICCF has a need to provide routine use of flow and imaging cytometry technology and application techniques in order to support over 250 basic and clinical research projects in 45 NINDS and other intramural programs. The new light sheet imaging system is required for development of innovative 3D multispectral imaging applications of whole organ tissues (i.e., whole mouse and rat brains after tissue clearing). This is a highly complex system combining high-throughput multispectral whole organ imaging at subcellular level of spatial resolution. The purpose of this acquisition is to acquire a state-of-the-art light sheet microscope system that would best meet the current and future facility requirements to optimally support intramural and collaborative research at NINDS.

3. Purchase Description: Multispectral Light Sheet Microscope System

4. Salient Characteristics: NINDS FICCF has a need for a state-of-the-art multispectral light sheet microscope system that would best meet the current and future facility needs for 3D multispectral imaging applications of whole organ tissues (i.e., whole mouse and rat brains after tissue clearing) in order to optimally support current and future intramural and collaborative research at NINDS. The system must meet the following required technical specifications:

a) full UV-NIR light spectrum excitation capability that includes at least 7 of the following laser line options 375 nm, 405 nm, 450 nm 488 nm, 561 nm, 592 nm, 647 nm, 730 nm and/or 785 nm including the availability of other wavelengths using a supercontinuum light source, with up to 7 simultaneous lines available per single scan

b) full UV-NIR light spectrum emission detection capability with commonly used fluorophores including DAPI, Alexa Fluor 405, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 594, Alexa Fluor 647, IRDye 680LT, IRDye 800CW, and PerCP

c) high-resolution (2048 x 2048 pixels or larger; 6.5x6.5 mm/pixel size or smaller), high- QE (75% or higher peak QE), high dynamic range (14-bit or 16-bit) CCD or sCMOS camera(s) with large field of view (18 mm or larger sensor diagonal) imaging, and optional support for ORCA-Fusion BT sCMOS camera for large specimen scanning

d) detection of up to 10 imaging fluorescence channels per specimen scan, including the option to iteratively scan the same specimen multiple times with automated image registration of the separately scanned multi-channel image datasets at the subpixel level

e) support for fully automated multiple specimen imaging (i.e., up to 5 whole mouse brains per batch scanning session), including large specimen imaging (i.e., whole monkey brain) up to 15 x 45 x 25mm3 that can fit into a large imaging chamber up to 130 x 55 x 65mm3

f) automated support for multiple high-NA microscope objectives optimized for light sheet imaging including 1X, 4X and 12X with zoom magnifications from 1X-30X range, large working distance up to 16 mm, and a large field of view up to 33 mm diagonal

g) optimized light sheet optics including adjustable light sheet NA and width for high-resolution, high-quality deep cleared tissue imaging at subcellular resolution with minimal illumination, photobleaching and spectral bleedthrough artifacts

h) compatibility with specimen imaging in both aqueous and organic solvents, with high refractive index matching spanning 1.33-1.56 range, and with the option to exchange samples without the need to drain the imaging chamber

i) user-friendly specimen setup and image acquisition software with software controlled on the fly chromatic correction of signal detection across full spectral range spanning 400-850 nm during specimen scanning

j) integrated image processing software for seamless 3D montage image stitching, image artifact correction (i.e., vignetting, photobleaching, autofluorescence, background fluorescence), shadow/stripping correction, chromatic correction, spectral unmixing, 3D image reconstruction, rendering, deconvolution, and extended depth-of-focus

k) integrated visualization and analysis software including algorithms for compound whole cell masking and feature extraction using separate image channels of subcellular compartments, standard and user-customizable machine learning/deep learning capabilities, and robust statistical data analysis, visualization and presentation tools (including movie and VR presentations of 3D montaged imagery)

l) data analysis import/export compatibility with flow cytometry standard (FCS) and image cytometry experiment (ICE) formats compatible with 3rd party flow cytometry and image analysis and presentation software (i.e., FCS Express, FlowJo, Kaluza, etc.)

4. Quantity: 1 Light Sheet Microscope System

5. Delivery Date: Delivery and installation within 30 days of purchase.

6. Warranty: Contractor shall provide a one year from date of installation warranty on the system. All service performed on this equipment shall be provided by certified and factory trained technicians. Contractor shall provide any required replacement components due to malfunctions on-site and will include all travel, parts and labor.

7. Delivery Requirements: The Contractor shall deliver and install the required equipment in coordination with Government officials. Delivery must be FOB Destination and must include inside installation. Delivery, installation and training must be performed at Building 10, Room 5S241, Bethesda, MD 20892 between the hours of 8:00 am and 5:00 pm, local prevailing time, Monday through Friday.

Multispectral Light Sheet Microscope System