Attachment 1 - Purchase Description - Inverted Microscope.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

ZEISS Axiovert 5 Digital Microscope with with Integrated Microscopy Mono Camera and Labscope Software or Equal

1. Statement of Need and Purpose: The purpose of this requirement is to procure one Zeiss

Axiovert 5 digital w/ integrated monochrome camera or equal.

2. Background Information and Objective: The Receptor Biology Section (RBS) characterizes the molecular mechanisms underlying neurotransmitter receptor transport and localization at the synapse. The lab requires a new inverted microscope and camera to replace an existing cell imaging system which sustained water damage in a recent flood.

3. Generic Name of Product: Inverted microscope

4. Purchase Description: ZEISS Axiovert 5 Digital with integrated Microscopy Mono Camera and

Labscope software or equal

5. Salient characteristics

a) must have integrated LED light sources for both transmitted and fluorescent light (385nm, 470nm, 565nm, 625nm) with > 60K work hours (except 385nm > 40K)

b) must include an option to automatically switch off and preserve integrated LEDs

c) must have a 0.4 NA condenser with light shield to block ambient light

d) must have a 6-position fluorescence filter turret, encoded for automatic detection, with options for both single-band and multi-band filter sets

e) must have a 6-position objective nosepiece, encoded for automatic detection

f) must include objectives corrected for 1-mm polystyrene for optimal use with standard plastic laboratory consummables

g) must include an integrated monochrome research camera with global shutter CMOS sensor, at least 5 megapixels, at least 3.45μm x 3.45μm pixel size, 350-1000nm spectral range, approximately 10500e full well capacity, 12-bit digitization, temperature stable at 25°C for ambient temperatures between 18°C and 30°C

h) must have a coarse/fine focus drive with 13mm of range and adjustable focus stop

i) must have a flexible manual XY stage that can fit microwell plates, flasks, Petri dishes d=36-95mm, object slides 76x26mm, and chambers (ibidi µ-SlideTM, Nunc Lab-TekTM I/II)

j) must have an image snap button on stand for ergonomic usage

k) must have one-click cell counting that calculates statistics for multiple fields

l) must have one-click confluency measurements that calculates statistics for multiple fields

m) must have live image measurement and annotation

n) must include software with intensity histogram, adjustments for gamma, brightness, contrast, color, and sharpness, over-exposure indicator, split view, multi-channel view, automatic detection of objective and filter set position, annotation, scaled measurements, file management, and options to save as image types CZI, JPG, and TIF

o) must have a reporting function to Word and PDF formats

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

p) must have a tablet mount that is upgradable to future-proof the microscope against tablet upgrades and is height/tilt adjustable for ergonomic usage and space management

q) must have a clamp to protect against tablet theft

r) must have a small footprint (maximum 363mm width, 503 mm depth, 505mm height) and recessed design for wetlab/bench compatibility

s) must have a modular build to allow for upgrades

6. Quantity: 1 (one) system

7. Delivery, Installation and Training: As soon as possible

The Contractor shall deliver and install the equipment within 45 days after receipt of order.

Delivery must be Freight on Board (FOB) Destination and include inside delivery. Training must take place within two (2) weeks of equipment installation. The equipment shall be delivered and installed in coordination with the Contracting Officer’s Representative (COR).

The contractor shall provide an Original Equipment Manufacturer (OEM) certified service technician to perform setup, installation, and an on-site 2-day training session for staff members. The contractor shall also provide one day of follow up training with Applications Specialist within 30 days of equipment installation.

Delivery Address:

Bldg 35 2C 907 9000 Rockville Pike Bethesda MD 20892

8. Other Requirements

The Contractor shall provide an Original Equipment Manufacturer (OEM) certified service technician to perform setup and installation.

Warranty - The Contractor shall warrant that the equipment will be free from defects for a period of at least 12 months from the date of installation, inspection by the Government, and acceptance.