Attachment 1 Purchase Description.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The National Institute of Neurological Disorders and Stroke (NINDS), Synaptic Transmission Section (STS) performs fluorescence protein-based experiments for 1) imaging the nanostructures at the cell membrane and 2) tracking the dynamics of a variety of proteins during vesicle exocytosis and endocytosis in the cells. These experiments need to resolve sub-10 nm range of proteins and membrane, which require ‘Super-Resolution Microscopy’ with Minimal Photon Fluxes (MINFLUX).

2. Background Information and Objective:

Synaptic Transmission Section combines physiological and cell biological approaches to understand the dynamics of cell membrane and a variety of proteins during vesicle exocytosis and endocytosis in living cells at nanometer scale, e.g. dense core vesicle docking, curvature generation and endocytosis. Our mission is first to characterize these processes with super-resolution imaging technique, and then determine the underlying regulating molecular mechanisms involved in neurodevelopmental and neurodegenerative disorders.

3. Generic Name of Product: 3D MINFLUX microscope

4. Purchase Description:

3D MINFLUX microscope with two MINFLUX Lasers (561nm and 642nm) a molecular localization precision of a few nm in all 3 dimensions with adaptive optics including an active sub nm accuracy sample stabilization in xyz (3D autofocus module) including an IX83 microscope body and multiple imaging modalities, four confocal excitations at 405nm, 485nm, 561nm, and 640nm - including full autoalignment and 5 detection channels.

5. Salient characteristics:

The following product features/characteristics are required for this requirement:

• MINFLUX technology with 3 nm localization precision, EOM-scanning localization via excitation minima using a doughnut-shaped excitation beam and nanometer-precision tracking at up to 100 kHz

• Inverted microscope body

• Scanning stage with travel range 125 x 75 mm, velocity up to 120 mm/s, resolution 0.1 µm

• Four-mirror Quad scanning with four galvo scanners and four galvo servo controllers with high pointing stability, with scan lens-free design for minimal wavefront distortions

• Optics package with full auto alignment of all beams and pinholes

• Deformable mirror unit for precise and fast z-positioning of all beams

• Active sample stabilization with nanometer precision in all three dimensions

• Ultra-fast (> 10 kHz) beam-scanning with << 1 nm precision within 400 nm – 950 nm wavelength range

• Direct output of the photon counts for quantitative measurements

• Eight gating channels for single-photon counting with < 80 ps time resolution

• Interleaved multi-channel acquisition as pulse and/or pixel and/or line-interleaved Direct output of the photon counts for quantitative measurements

• Workstation PC (min. 16 GB RAM, Intel Core, graphics GTX670 or equivalent) with software package for Data acquisition (software package for MINFLUX tracking acquisition mode) and Analysis (incorporating automatic control of imaging, automatic measurements, toolbox for quantitative microscopy, color-encoded display of quantification and tracking, and GUI with customized workflows); Monitor (>= 32 TFT)

• High-power laser modules for 561 nm and 642 nm with > 1000 mW power and power stability < 2% for at least 8 hrs, with option of using different objective lenses (oil, water, silicone, glycerol).

• Beam-shaping modules for 561 nm and 642 nm with spatial light modulator for dynamic beam shaping, capable of forming doughnut-shaped STED beam with correction for sample distortions

• Activation laser module in MINFLUX mode with ultrafast digital modulation

• Objectives: UPlanSApo 100X/1.40 Oil, UplanSApo 60X/NA 1.2 water, UPlanSApo

20X/NA 0.85

• Widefield Module for Epifluorescence

• High sensitivity CMOS epifluorescence camera for epifluorescence detection ( Orca

FLASH 4.0 V3 or equivalent with peak quantum efficiency of >= 80% at 560 nm, 6.5 x

6.5 μm cell size)

• Laser modules for 485 nm, 561 nm, 642 nm, 405 nm

• Three detection channels with Single-photon counting APD with a superior sensitivity of up to 65% detection efficiency and low dark count (< 250 Hz).

• Optical Table 1800 x 1250 x 55 mm with actively leveled isolation frame and breadboard.

• Optical enclosure for background-free imaging

• On-site customer training

6. Quantity: 1 system

7. Delivery and Installatin requirements:

The contractor shall deliver to National Institutes of Health within 22 weeks after receipt of the order award. Then the contractor shall provide delivery and relocation of the device from shipping receiving storage to final site location and provide installation of the equipment within 70 days after receipt of order. Site location address is Building 35, Room 2BC-909, 35 Convent Drive, Bethesda, MD 20892-3706.

8. Warranty Requirements:

Contractor shall warrant that the Equipment will be free from material defects for a period of twelve (12) months from the date of acceptance. All service performed on this equipment shall be provided by certified and factory trained technicians. Technicians shall have the ability to diagnose the systems on site via remote access. Contractor shall provide any required replacement components due to malfunctions on-site. Warranty shall not include periodic maintenance services.