Attachment 1 Purchase Description.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The Neuromuscular and Neurogenetic Disorders of Childhood Section at NINDS studies cellular and animal models of neuromuscular diseases and is in need of a confocal fluorescence imaging system with the specifications described in this document.

2. Background Information and Objective:

At the Neuromuscular and Neurogenetic Disorders of Childhood Section, within the Neurogenetic Branch of the National Institute of Neurological Disorders and Stroke, the lab is developing cellular and animal models of early onset neuromuscular diseases, including diseases in which the primary defect is located at the neuromuscular junction, the myomatrix, or within the myofiber.

Our two main focuses are to 1) study disease pathomechanisms and 2) conduct preclinical testing of gene- and transcript-directed therapeutic strategies. Our ultimate goal is to translate innovative therapeutic approaches from the bench to the bedside, and to provide disease-modifying therapies to patients affected with these rare conditions.

Confocal microscopy allows imaging of multiple fluorescently labeled epitopes within cells and tissues. This experimental approach is instrumental to our studies, to visualize structures in situ and assess localization of specific proteins of interest. Our current instrument, the Leica TCS SP5, is a confocal system with optoacoustic splitter and hybrid detectors, used for variety of applications including some limited live imaging. We are looking into upgrades to this system to include super resolution imaging, 3D imaging options, and lifetime/live imaging sensors. These upgrades are necessary to address some of the challenges we are facing with our current system (limited sensitivity and number of probes, lack of 3D reconstruction, limited live imaging, etc.).

3. Generic Name of Product:

Confocal laser scanning microscopy system

4. Purchase Description and Salient Characterisitics:

This requirement will be for brand name or equal items as described in this document.

The vendor shall provide equipment brand name or equal to Leica Microsystems Stellaris 8 with the following modalities:

• STED Scan Head with Leica's Exclusive Acousto-Optical-Beam-Splitter (AOBS) o Filter-free dynamic beam splitter AOBS® manages up to 8 lines simultaneously o High transmission (>95%) across full visible spectrum o Automatically adapts to all laser lines selected, including any combination of 8 simultaneous lines

• White Light Laser (WLL) o Eight (8) simultaneous excitation lines between 440nm and 790nm o Each line tunable in 1nm increments o Dynamic beam splitter AOBS® instantly adapts to each laser line (up to 8 simultaneously) o Pulsed laser for lifetime imaging with all Power HyD® detectors

• High-Speed 8K Resonant Scanner with Pixel-Clock Control for scanning feedback and Optimization o Scan formats up to 2,496 x 2,496 o Continuous Zoom and Scan Field Rotation o 28 fps x 5 simultaneous channels = 140 images per second at 512 x 512

• Tunable-Speed Conventional Scanner o Linear Scan Mode o Scan speeds up to 2,600 Hz, 5,200 Hz Bidirectional o Scan formats up to 8,192 x 8,192 o Continuous Zoom and Scan Field Rotation o 10 fps x 5 simultaneous channels = 50 images per second @ 512 x 512 o 22mm FOV scanner with continuous zoom and scan field rotation

• Five (5) Simultaneous Spectral SP® Detection Channels based on Leica's High- Effifficiency Pellin-Broca Prism o Power HyD S, Positions 1,3, and 5 New silicon-based, solid state detector with >56% Photon Detection

Efficiency (PDE) Increased sensitivity in the NIR up to 850nm >2-fold increase in dynamic range over previous generation HyD detectors Analog and Photon Counting modes with PowerCounting o Power HyD X, Positions 2 and 4 Leica's GaAsP based detector optimized for STED, functional imaging with FALCON FLIM and FCS High efficiency and extremely low noise from 410nm to 750nm Analog and Photon Counting modes with PowerCounting

• TauSense modalities o TauContrast o TauSeparation o TauGating

• TauSTED Nanoscopy for 2D and 3D SuperResolution o STED Scanner module with Auto Beam Alignment o STED depletion laser 775nm pulsed (upgradeable to 592nm and 660nm depletion) o TauSense based TauSTED capability for low laser power nanoscopy with fixed and live cells

• Premium CUDA Workstation with high-end GPU for accelerated 3D image display and

Processing

• Leica Lightning for Near Real-Time Confocal SuperResolution and Image Detail

Extraction o SuperResolution with resolution to 120nm lateral and 200nm axial o Lighting & Dynamic Signal Enhancement with Conventional and Resonant

Scanning o for maximum S/N and SuperResolution without compromising Speed or FOV o Compatible with all microscope objectives

General Requirements

• Generate high-resolution 2D and 3D images of skeletal muscle structures (including the neuromuscular junction, the myomatrix and the myofibers), for the purpose of detecting and quantifying changes to these structures in several models of neuromuscular diseases and under various therapeutic paradigms

• Perform live imaging in cellular models, for the purpose of imaging and quantifying pathways involved in the pathogenesis of these diseases;

• Support a high-throughput workspace, for the purpose of drug screening.

• A tandem scanner with continuous zoom and scan field roation including

• a high-speed 8K resonant scanner (up to 140 images per second),

• a tunable-speed conventional scanner (up to 5,200 Hz bidirectional), high resolution (up to

8,192 x 8,192 scan formats)

• White light laser that allows up to 8 simultaneous excitation lines (in the 440nm to 790nm range),

• each tunable in 1nm increments

• Five detection channels (high sensitivity HyD S (3) and HyD X (2) detectors)

• TauSense family of modalities to generate high-contrast, multiplexed and detailed images

• Leica Lightning for SuperResolution and image detail extraction

• Leica Super Z Galvo Stage for high-speed Z series and live XZ scanning

• Five objectives

• Installation and Training

Compatibility Requirements

• The system will come with its own parts, computer and software. We don’t currently have pieces of equipment or IT that will be compatible with this new system.

5. Trade in Requirement:

The following government owned equipment will be traded in as part of this requirement;

• Leica SP5, Serial Number: 5100001590

6. Other Requirements:

The Contractor shall provide an Original Equipment Manufacturer (OEM) certified service technician to perform setup and installation for staff members.

Warranty - The Contractor shall warrant that all computer equipment (computer, monitor) will be free from defects for a period of at least three (3) years from the date of installation, inspection by the Government, and acceptance. The Contractor shall further warrant that all optical and mechanical components will be free from defects for a period of at least five (5) years from the date of installation, inspection and acceptance by the Government. Warranty service shall include trouble-shooting capabilities based on complete knowledge of the entire system. Warranty shall not include routine or periodic maintenance services.

7. Quantity:

One (1) microscope system

8. Delivery, Installation and Training:

The Contractor shall deliver and install the equipment to the NIH in North Bethesda, Maryland within 90 days after receipt of order. The equipment shall be delivered and installed between the hours of 8:00am and 5:00pm EST, Monday through Friday. Delivery must be Freight on Board (FOB) Destination and include inside delivery. The equipment shall be delivered and installed in coordination with the Contracting Officer’s Representative (COR) at the time the equipment is ready to be shipped.

Contractor shall provide on-site training within 30 days weeks of equipment installation.

Contractor shall deliver, install, and provide training on the system within 120 days after receipt of order.

9. Period of Performance: N/A

10. Option(s): N/A