Attachment 1 - Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Lasers for Multiphoton Microscope – Dr. Yi Gu Spatial Navigation and Memory Unit

(SNMU), NINDS

1. Statement of Need and Purpose:

The Spatial Navigation and Memory Unit needs two fixed-wavelength (920 nm and 1064

nm) compact femtosecond lasers to perform research studies focusing on the synaptic plasticity in the medial entorhinal cortex (MEC) during spatial learning. Specifically, fixed wavelength femtosecond lasers at 920 nm and 1064 nm are essential for imaging synaptic surface dynamics of GluA1 using GluA1-SEP and calcium dynamics of the

MEC neuron using jRGECO, respectively. These two wavelengths are known to optimally activate these indicators. In addition, the lasers should be compact to fit our small air table, and both lasers should have built-in total power control (TPC) and dispersion compensation.

2. Background Information and Objective:

Dr. Yi Gu heads the Spatial Navigation and Memory Unit (SNMU), which aims to investigate the neural basis pf spatial navigation and memory. Specifically, the experiments in the laboratory plans to use in vivo two-photon imaging approach to measure calcium dynamics of the MEC neurons while observing their structural and molecular properties at the subcellular level. The laboratory will purchase a two-photon laser scanning microscope system to achieve the above measurement. For this microscope system, the laboratory will require two fixed-wavelength (920 nm and 1064

nm) compact femtosecond lasers. The collected data using entire system, including the microscopes and the two lasers, are important to understand the synaptic plasticity of the MEC in spatial learning.

3. Generic Name of Product:

Lasers for multiphoton microscope

4. Product Description:

This requirement is issued on a brand name or equal basis to:

(1) Trade name: Axon 920-1 TPC and Axon 1064-1 TPC

(2) Product’s brand name: Axon 920-1 TPC and Axon 1064-1 TPC

(3) Manufacturer’s name: Coherent Inc.

(4) Model: Axon 920-1 TPC and Axon 1064-1 TPC

(5) Catalog number: 1403616 and 1403618

5. Salient characteristics

The NINDS SNMU requires a combination of two fixed-wavelength (920 nm and 1064

nm) compact femtosecond lasers, which must have the salient features listed below:

Minimum features of the lasers:

Simultaneous laser outputs at two wavelengths: 920nm and 1064nm

Integrated dispersion precompenstation of 920nm laser: 0-22000 fs2

Integrated dispersion precompenstation of 1064nm laser: 0-13000 fs2

Average power: 800 mw

Pulse duration: < 150 fs

Repetition rate: 80 MHz

Beam mode: M2<1.3

Beam asymmetry: 0.8 to 1.2

Beam diameter: 1.2 ± 0.2 mm

Power stability: ± 0.5%

Noise (%): <0.25

Polarization: >100:1 vertical

Manipulation rise/fall time: <1000ns

Contrast ratio: > 1000:1

Laser head dimensions: 212 x 318 x 62 mm

Umbilical length: 7m

Laser power supply dimensions: 3U, 19” rack mount unit

Operating temperature range: 19 to 26°C

Relative humidity: <85. Non-condensing

Power requirements: 100/240 VAC

Control interface: RS-232 or USB

Synch output: BNC, 50% duty cycle, 3.5 V into 50 Ω

Analog power control 0-5 V

Fully integrated power modulation via fast AOM control

Built-in total power control

6. Quantity:

Two (2) lasers

7. Delivery Date:

The contractor shall deliver and install the equipment within 60 days after receipt of order. Delivery must be FOB destination and include inside delivery. On-site training must take place within two (2) weeks of equipment installation. The equipment shall be delivered and installed between the hours of 8:00am and 5:00 pm, Bethesda, MD local prevailing time. The contractor shall provide an Original Equipment Manufacturer

(OEM) certified service technician to perform setup, installation, and an on-site 1-day training session for 10 staff members.

8. Warranty Requirements:

Contractor shall warrant that the Equipment will be free from material defects for a period of twelve (12) months from the date of acceptance or fourteen (14) months from the date of Delivery if installation is delayed through no fault of Company, whichever occurs first.

9. Option(s):

N/A