Attachment 1 Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The goal of our brain imaging studies at the National Institute of Mental Health (NIMH), Molecular Imaging Branch is to advance the notion of quantitative measures in the diagnosis of psychiatric illnesses. Such an approach relies heavily on specific target brain receptor imaging. An important part of our research and development studies is the screening and selection of newly synthesized compounds that show selectivity and good affinity to designated brain receptors. Compounds that have favorable properties are then radiolabeled with the short half-lives (11C-20 min and 18F-110 min) radionuclide for preclinical animal evaluations. The most favorable properties that is measured in vitro is by the competitive binding assays. The synthesized compounds are made to compete against a known receptor specific ligand. These ligands are commonly radiolabeled with tritium (3H-). The only methods that may detect the 3H-radioactivity and thus help gather the necessary assay results is a liquid scintillation counter. Therefore, the purpose of the requested Beta Microplate Reader system is to allow the reliable detection of the 3H-radioactivity.

2. Background Information and Objective:

The Molecular Imaging Branch current liquid scintillation counter (Tricarb-3100TR), which is in building 49 (Rm B1A59) in our in vitro binding assay lab, is over 16 years old.

This instrument is no longer supported by the manufacturer. A new replacement system is needed which allows performance at a higher throughput study. The old system has been used efficiently with protocols that were optimized to allow efficient procedures, but an improvement in a higher throughput studies with shorter time between start and obtaining useful results is needed.

The requested Beta Microplate Reader instrument will be used to shorten the assay time and increase the throughput studies. Furthermore, although the MicroBeta system is a completely different technology and assay system, it will still allow us to use our existing protocols for comparative purposes and to allow secure switching to the new instrument.

These studies include, but are not limited to, the investigations of selecting promising compounds that we synthesize and are targeting in human cyclooxygenase 1 and 2 (COX-1 & 2). The efficient assays for these two targets would be important to support our proposed protocols in finding compounds to radiolabel with 11C- and 18F- and perform positron emission imaging (PET) studies. Thus, the goal of our work is the successful research and development of brain receptor imaging.

3. Generic Name of Product: Beta Microplate Reader.

4. Purchase Description: MicroBeta2 Plate reader and a Cell Harvester

*MicroBeta2 Plate Reader with 2-detectors Quantity: 1 EACH

Salient characteristics:

-TRLSC and coincidence counting -16 Plate capacity

- Internal PC with Windows 10 -LCD display -Keyboard -Mouse -MicroBeta2 software -10 pcs of rigid 96-well cassettes -10 pcs of rigid 384-well cassettes ( -100 pcs ID Support Plates (for attachment of bar code labels) -ID labels -MicroBeta2 Instrument Manual (English) -MicroBeta2 User Manual (English) -MicroBeta2 Quick Guide (Multilingual) -roller for sealing plates -Starter kit for MicroBeta

1 bottle of MicroScint-PS 1L 1 bottle of Super Mix 1L 1 (Microbeta plate starter kit):

5 EACH (96 well Flexible Plates) 5 EACH (24 well Flexible Plates);

2 EACH (Isoplate 96 well format, white and clear bottom) 20 EACH (Sealing Tape, permanent) 20 EACH (Sealing Tape, removable)

*Installation Kit_100, 115, 120, 240 V (+/- 10%), 50/60 Hz Quantity: 1 EACH

*Microbeta Normalization Standard Quantity: 1 EACH

*Cassette for 4ml Mini Vials Quantity: 1 PACKAGE

*4ml MicrobetaI Vial Quantity: 1 PACKAGE

*New Instrument Online Training & Support

*Installation II

*Cell Harvester for MB Filtermat-96 well Quantity: 1 EACH

Salient characteristics:

-Short fluid path -Separate hot and cold waste lines -Built-in scrubbers

*Wash Bottle & Tubing Kit Quantity: 1 EACH

*Vacuum Pump Kit, 110V Quantity: 1 EACH

*New Instrument Online Training & Support

5. Delivery Date: Thirty days after receipt of order.

6. Period of Performance: N/A

7. Warranty: one year (parts, labor, and travel)

8. Option(s): N/A