Attachment 1 - Purchase Description_AKTA Go or equal.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The purpose of this acquisition is to procure a chromatography system for purification of proteins used in biochemical studies of the tubulin/microtubules and their regulators. The laboratory requires a new purification system to replace a chromatography system for purification of proteins that was damaged.

2. Background Information and Objective:

The National Institutes of Health (NIH) is the nation’s leading medical research agency and the primary Federal agency whose mission is to seek fundamental knowledge about the nature and behavior of living systems and the application of that knowledge. The mission of the NIH also includes enhancing health, lengthening life, reducing illness and disability, conducting, supporting and making medical discoveries that improves and saves people’s health and lives.

The Cell Biology and Biophysics Unit (CBBU) conducts biophysical and structural studies on microtubules and microtubules regulating proteins with focused on understanding the function of post-translational modifications on the recruitment of cellular effectors. The required chromatography system is needed for preparation of proteins used on daily basis for multiple ongoing research projects.

3. Generic Name of Product:

Chromatography system for purification of proteins.

4. Purchase Description:

a) Product’s brand name: AKTA Go Chromatography System or Equal

b) Manufacturer's name: Cytiva Global Life Sciences Solutions USA LLC (formerly GE

Healthcare)

c) Model, type, catalog, and/or part number(s): AKTA Go with Unicorn software and components or equal (Part Nos. 29383015, 29702890, 28956500, 28656342, 28956327, 18111089, 29011367, 28956282, 29454032, 29476924, 29435648)

5. Salient characteristics The chromatography system shall meet the following requirements:

a) footprint of 335 × 464 mm (width × depth) and height 705 mm with of F9-T tunnel, to fit in the cold cabinet space.

b) Fraction collector must be compatible with a range of deep-well plates, microplates, and small tubes. F9-T can be placed in a tunnel underneath the system for optimization of cold-room space.

c) UV monitor is silent and eco-friendly with low power consumption and long life span. Requires no warm-up prior to runs and does not heat samples

d) In order to control biomolecule separations, a conductivity detector that functions in the range of one mS/cm to 999.9 mS/cm is needed

e) System have to work with up to 25 ml/min flow rate and pressure up to 5MPa to support commonly used chromatography techniques

f) Because we will be using salt buffers, the entire wetted flow path of the system must be inert to halides. The pump heads must be titanium. This stops the buildup of hydrophobic products within the pump head. All valves and tubing must be

PEEK.

g) The pumps, UV, conductivity monitors, valves and fraction collector must all be specified for use in a cold room at 4 degrees C.

h) System must be equipped with static mixer, which requires low maintenance due to no moving parts

i) The system must have modular design, which brings versatility and flexibility in using the system. Additional parts/modules can later be added on to further expand the system capability and productivity.

j) The platform shall contain a sample inlet valve

k) The unit shall contain air sensor, automatically detecting and prevent air from entering columns and enable the safer exclusion of air from the system.

l) The system must be control by software UNICORN version 7.0 or later (or equal), which provides a fast and simple start to creating methods and starting runs, easy protocol transfer during scale-up and an improved visual interface for intuitive and flexible method editing. It also allows of easy access to manual controls even during method runs.

m) The chromatography system must be compatibility with existing equipment (AKTA Pure 25 M1) and the associated chromatography columns, connecting parts and tubing used in the laboratory.

n) Installation shall be provided by a manufacturer certified service technician.

o) On-line/remote training

p) Technical Support available on site and via phone/email with Protein Purification

Applications Scientist

q) Warranty extension for an additional 48 months for unlimited service repair coverage (travel, labor and parts)

6. Quantity: One (1) AKTA Go chromatography system or equal with associated components

7. Delivery Date:

Delivery must be completed within 10-15 days after contractor receipt of award.

Installation and training must be completed within six (6) weeks after system delivery.

8. Delivery Requirements: The Contractor shall deliver and install the equipment in coordination with Government officials. Delivery must be FOB Destination and must include inside installation. Delivery, installation and training must be performed at 35 Convent Drive, Room 3B205, Bethesda, MD 20892 between the hours of 9:00 am and 5:00 pm, local prevailing time, Monday through Friday.

9. Other Requirements:

The Contractor shall provide an Original Equipment Manufacturer (OEM) certified service technician to perform setup and installation.

Contractor shall warrant that the equipment will be free from material defects for a minimum period of twelve months from the date of installation, inspection by the Government, and acceptance. All service performed on this equipment shall be provided by certified and factory trained technicians. Contractor shall provide any required replacement components due to malfunctions on-site.