Attachment 1 Product Description NIMH20003894RFQ.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The NIMH Systems Neuroscience Imaging Resource

(SNIR) requires a new slide scanning microscope. The purpose is to allow SNIR users to program unattended image acquisition from multiple tissue sections on multiple slides.

2. Background Information and Objective: NIMH IRP investigators who use the SNIR perform experiments in which they need to collect light microscope images of very large amounts of sectioned material. This is frequently of entire brains from mice, rats, marmosets and macaques. The material is cut into sections that may be 20-60 microns thick and mounted onto slides. Depending on the species, the section thickness, and whether all sections or a sampling are used, a brain may create from 20 to several hundred sections. It may be necessary to image them in either brightfield or fluorescence. Depending on details of the experimental information required, it may be appropriate to image at relatively low magnification (2.5-5X), medium (10-20X), or high

(40X) magnification, or to initially image at low magnification and subsequently image selected regions of interest at higher magnification. It may be appropriate to collect images from a single focal plane or to collect images from multiple focal planes at each position. For some experiments widefield imaging does not provide sufficient contrast or axial resolution in all parts of an image and in these cases confocal reimaging selected parts of the image is desirable. The goal of this acquisition is to purchase an instrument that meets these needs in a technically excellent manner and with a user-friendly interface.

3. Generic Name of Product: Widefield and Confocal Slide Scanning Microscope

4. Purchase Description:

Slide scanning system including capacity for 1x3 and 2x3 inch slides, capable of widefield and confocal fluorescent imaging and brightfield imaging, with low and high magnification objectives, and an anti-vibration table.

5. Salient characteristics:

1) Includes holders for glass slides that are 1 x 3 inches (minimum 120).

2) Provides brightfield, phase contrast and fluorescence illumination.

3) Accommodates objectives from 2X to at least 40X, holding at least 6 at one time.

4) Includes 2X, 4X, 10X, 20X, 40X/0.75 NA and 40X/0.95 NA , dry objectives or equivalent.

5) Software provides automatic sample identification, focus mapping, stitching, separate control of acquisition parameters (objective, illumination colors, emission filters, z-stack parameters), and marking of regions of interest on low magnification scans for rescan at higher magnification.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

6) LED illuminator with at least 6 peaks at covering the range from approximately 390 to 730 nm.

7) Able to image and discriminate DAPI, Alexa 488, Alexa 594, Cy5, and Cy 7, adaptable to image and discriminate other fluorophores.

8) Capable of imaging fluorescent samples in either widefield or confocal modes.

9) Anti-vibration air table that accommodates the system.

10) Following delivery and installation, no less than 16 hours of on-site training that is necessary for the routine operation of the microscope.

6. Quantity: One

7. Delivery Date: 60-120 days following the award of the contract

8. Period of Performance: All items fully warrantied for one year. Equipment is expected to be fully functional for greater than 5 years with minor service, as required.

Maintenance service after one year may be purchased as an annual contract or on an as needed basis.

9. Option(s): None