Attachment 1 NIMH20003934 RFQ- Product Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The Laboratory of Neuropsychology (LN) and

Laboratory of Brain and Cognition (LBC) require a new slide scanning microscope. The purpose is to allow LN/LBC users to program unattended image acquisition from multiple tissue sections on multiple slides.

2. Background Information and Objective: LN/LBC investigators perform experiments in which they need to collect light microscope images of very large amounts of sectioned material. This is frequently of entire brains from marmosets and macaques. The material is cut into sections that may be 20-40 microns thick and mounted onto slides. Depending on the species, the section thickness, and whether all sections or a sampling are used, a brain may create several hundred sections. It may be necessary to image them in either brightfield or fluourescence. Depending on details of the experimental information required, it may be appropriate to image at relatively low magnification (2.5-5X), medium

(10-20X), or high (40X) magnification, or to initially image at low magnification and subsequently image selected regions of interest at higher magnification. It may be appropriate to collect images from a single focal plane or to collect images from multiple focal planes at each position. The goal of this acquisition is to purchase an instrument that meets these needs in a technically excellent manner and with a user friendly interface.

3. Generic Name of Product: Slide scanning microscope.

4. Purchase Description:

A) Slide scanning microscope system with the following capabilities:

i) Capacity for scanning 1x3 and 2x3 inch slides in X, Y and Z.

ii) Objectives with 2X, 4X, 10X, 20X and 40X magnification or similar.

iii) LED fluorescent illuminator capable of efficient excitation of dyes ranging in excitation maximum from approximately 405-700 nm

iv) Configured for imaging DAPI, Alexa 405, Alexa 488, Alexa 555, Alexa 594, Alexa 647, Cy7.

v) Separate control of excitation and emission filters to provide for imaging long Stokes shift and novel fluorophores.

vi) Must work on 110V mains input.

vii) Software that provides: stitching, option of saving Z-stacks or projections.

B) Antivibration table with casters that accommodates the slide scanning microscope system.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

5. Salient characteristics:

1) Accommodates glass slides that are 1 x 3 inches (minimum 200) and glass slides that are 2 x 3 inches (minimum 100).

2) Provides brightfield, phase contrast and fluorescence illumination.

3) Accommodates objectives from 2X to at least 40X, holding at least 6 at one time.

4) Includes 2X, 4X/0.16 N, 10X/0.4 NA, 20X/0.8 NA, 40X/0.95 NA,

5) Software provides automatic sample identification, focus mapping, stitching, and marking of regions of interest on low magnification scans for rescan at higher magnification.

6) LED illuminator with at least 6 peaks at approximately 385, 430, 475, 545, 650, 735 nm.

7) Able to image and discriminate DAPI, Alexa 488, Alexa 594, Cy5, Cy 7, adaptable to image and discriminate other fluorophores

8) Color camera with software switching between monochrome and color cameras.

9) Anti-vibration air table that accommodates the system

10) Following delivery and installation, no less than 16 hours of on-site training that is necessary for the routine operation of the microscope

6. Quantity: One

7. Delivery Date: 60-120 days following the award of the contract

8. Period of Performance: All items fully warrantied for one year. Equipment is expected to be fully functional for greater than 5 years with minor service, as required.

Maintenance service after one year may be purchased as an annual contract or on an as needed basis.

9. Option(s): None