Attachment 1 20003895 - New Confocal PD.pdf

NIMH20003895RFQ Attachment 1

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The NIMH Systems Neuroscience Imaging Resource

(SNIR) requires a new microscope system to provide state of the art confocal microscope imaging.

2. Background Information and Objective: Imaging brain sections using confocal microscopy is an essential step for experiments that are being carried out by basic science investigators within the NIMH intramural program. The SNIR supports this by managing shared equipment used by multiple investigators. The SNIR requires a new microscope because current instruments are heavily used and because the state of the art has improved significantly over that used in any of the microscopes the SNIR now manages. To continue their ground-breaking research, IRP investigators need to take advantage of some of the recent technological advances. The goal of this purchase is to acquire a new microscope that incorporates features that will provide state of the art imaging capabilities that are relevant to the research projects of NIMH IRP investigators.

These include: i) rapid high-resolution imaging, ii) simultaneous imaging and discrimination of multiple fluorophores, iii) discrimination of fluorophores with overlapping or identical emission spectra, iv) imaging of fluorophores with excitation peaks that range from near 400nm to over 750nm and emission peaks that range from near 400nm to at least 800nm.

3. Generic Name of Product: Laser scanning confocal microscope.

4. Purchase Description:

Laser scanning confocal microscope system with a pulsed white light laser. Capabilities must include rapid high-resolution x/y/z imaging, discrimination of multiple fluorophores with excitation maximum that cover at least 405 to 730 nm and the possibility to discriminate fluorophores with overlapping emission spectra based on fluorescence lifetime.

5. Salient characteristics:

Confocal microscope system with:

1) 8 kHz tandem scanner

2) 405 nm Laser

3) Pulsed White Light Laser (WLL) with fully tunable 470-670 nm or greater range output and up to 8 laser lines simultaneously coupled to the scanner with an Acousto-Optical-Beam-Splitter (AOBS)

4) Minimum of five Internal and independent Spectral Detector Channels, including a minimum of 3 HyDs (or equivalent)

a. Filter free, prism-based spectral detectors, individually regulatable channels

NIMH20003895RFQ Attachment 1

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

b. Detectors must be time-gated and synchronized with the pulsed WLL

5) Continuously adjustable non-resonant scanner (1-1800 Hz)

6) Transmitted Light Brightfield Detector

7) XY Scanning Stage with optical feedback loop

8) Image resolution of up to 64 Megapixels (8,192 X 8,192)

9) Objectives including 5X, 10X/0.4, 20X/0.75, 40X/1.3 Oil

10) Navigator software (large area overview scanning with both confocal and camera) for fast acquisition of large areas of sample to define regions of specific interest for higher magnification acquisition. Must include an automatic focus map generation to handle tissue irregularities and sample vessels that are not flat. Must be able to define irregular area tile scans with different z stack parameters for each area.

11) Following System set up and installion by vendor, no less than 16 hours of on-site training that is necessary for the routine operation of the microscope

6. Quantity: One

7. Delivery Date: Between 60-120 days following the award of the contract

8. Period of Performance: All items fully warrantied for one year. Equipment is expected to be fully functional for greater than 5 years with minor service, as required.

Maintenance service after one year may be purchased as an annual contract or on an as needed basis.

9. Option(s): None