Questions_and_Answers.docx

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Next Generation Target Sequence Capture in Wheat Federal contract opportunity
Solicitation number
AG-0202-S-18-0011
Issued by
Department of Agriculture Agricultural Research Service Field Research Implementation and Information Delivery Pacific West Area

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DATE:09/19/2018
RE:AG-0202-S-18-0011 Next Generation Target Sequence Capture in Wheat
SUBJECT:Questions and Answers
Q:Could you specify the specific location of targeted region (i.e. the initial and terminal position of the gene)?
A:We do not know where the gene is located, only that it is in a 7 Mbp region between 675 and 681 Mbp on chromosome 2BL.

Q: Do you accept similar kits and equipment as you specified (such as using Agilent SureSelect to capture probes)?

A: Probably any probe technology that can be sequenced using Illumina’s HiSeq 4000 will work.

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