Specifications_for_Digital_Droplet_PCR_system.docx
DOCX document 13 KB Posted
- Attached to
- Digital Droplet PCR System Federal contract opportunity
- Solicitation number
- AG-0202-S-16-0006
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Specifications for Digital Droplet PCR system
1. 96-well throughput format, 20 microliter reaction volume; droplet generation time is rapid (several minutes or less); analysis time for droplet reader is 2.5 hrs or less.
2. Ability to count multiple copies in a single droplet
3. Ability to multiplex multiple targets
4. Reagent/consumables cost of $3 or less per sample
5. Sample amplification is performed on a standard thermalcycler
6. Capable of distinguishing a 20% difference in copy number of genes (that is, four vs. five copies)
7. Compatible with Taqman or EvaGreen chemistry (currently in use in our lab)
8. Small footprint, compatible with limited benchtop space (26 x 20 x 11" or similar size)
9. Capable of reading FAM, HEX, EvaGreen dyes
10. Comes with software capable of:
| -displaying fluorescence amplitude values per droplet for FAM, HEX, EvaGreen dyes |
| -shows multiplex data for up to two fluorphores per droplet |
| -Computes concentration and copy number of targets |
| -automatically sets significance thresholds, or allows user selected thresholds |
| -can merge results from replicate wells |
| -can calculate absolute copy number |
| -graphs and tables can be copied and pasted; data can be exported as .csv file |
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