A04_Specifications.pdf

Required Specifications

The USGS Eastern ecological Science Center is seeking to purchase an automated electrophoresis instrument for DNA and RNA sample quality control. The instrument requirements are as follow:

Must provide QC of RNA, genomic DNA (gDNA), cell-free DNA, NGS library, and PCR/qPCR fragment analysis

Sample consumption ≤2 µL) Analysis time ≤ 2 minutes per sample Low-throughput instrument with up to 16 samples per run, but can also run a single sample at one time without loss of reagents Small footprint ≤ 275 mm x 525 mm x 425 mm (WxDxH) Provides integrity standards for RNA (RIN) and genomic DNA (DIN) Quantitative range 25–10000 pg/μL of RNA 10-4000 pg/μL DNA Limit of detection ≤100 pg/μL RNA, ≤0.5 ng/μL DNA, ≤20 pg/μL cfDNA Minimum sample sizing range 100–6000 nt RNA, 200–60000 bp, DNA 50–800 bp cfDNA Instrument control via PC compatible data analysis and reporting software.

Results can be displayed as an electropherogram, gel image, or in tabular format.

Software must automatically determine sample size, quantity and molarity.

Reports can be saved as PDF or exported for to MS Word or Excel.