900009743 - Jamie Diemer - Custom TS-C Human Panel - 231025 - signed.pdf
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- Attached to
- TotalSeq™-C Custom Human Panel; 53 reactions Federal contract opportunity
- Solicitation number
- NHLBI-24-002830
About this file
This specification form details a request for a custom TotalSeq-C human antibody panel from BioLegend consisting of 53 reactions. The panel includes 111 antibody clones targeting various cell surface markers and intracellular proteins for human immune cell phenotyping and profiling. Key information provided in the specification form includes the intended research use of the final materials, packaging and logistics requirements such as vial types and documentation needs, and a spreadsheet listing each antibody clone, target specificity, assigned barcode, and recommended concentration per test. The form also identifies the requester as Chris Hourigan from the National Heart, Lung and Blood Institute of the National Institutes of Health. BioLegend will manufacture the custom panel to ISO 13485:2016 standards for research use only.
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Specification Form for Custom TotalSeq™ Panel Request This is not an order form – Please be sure to place your order with the appropriate contacts below.
CST015-F5/Form-02074 Specification Form for Custom TotalSeq™ Panel Request R3 Document No. Form-02074 R3 Effective Date: 7/15/2022
Date Printed 10/11/2023
BioLegend, Inc.
8999 BioLegend Way San Diego, CA 92121 Tel: 877.246.5343 Fax: 877.455.9587
Name and Institution: Chris Hourigan, NHLBI Date: 20230909
Email and Phone Number: Hourigan, Christopher (NIH/NHLBI) [E] <christopher.hourigan@nih.gov>
E-Signature:
US: Email: customorders@biolegend.com Phone: 877.246.5343 International: 1-858.768.5800 Fax: 877.455.9587 Europe: Email: infoeurope@biolegend.com Phone: +44 (0) 20 3475 3880 Fax: +44 (0) 20 3318 3271
A. General Information
Intended Usage of Final Materials
Research Use Only
ASR/IVD or other diagnostics
Resale/OEM
Other, please specify:
Oligo format
TotalSeq™-A
TotalSeq™-B
TotalSeq™-Bn
TotalSeq™-C
TotalSeq™-D
Other:
Sample Species Human Mouse Other:
Number of tests required Please note order minimum of 50 reactions
53 reactions
BioLegend custom products are manufactured in an environment certified to ISO 13485:2016 but not under design control guidelines. For Research Use Only. Not for use in diagnostic or therapeutic procedures. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
B. Packaging and Logistics
Will this be a repeat order?
No
Yes: please provide further details on total number of tests and order frequency:
Formulation and Storage Buffer Used
1X PBS, 1mM EDTA, lyophilized (standard)
Other, please specify
Packaging *Standard one-test vials should be reconstituted with 25µL of the antibody cocktail and 25µL of FcR blocked cells for a total staining volume of 50µL. This allows the staining of up to 5 x 105 cells.
Standard*, Lyophilized Single Test Vials, 5 vials per pouch
Other. Please specify desired staining volume, tests per vial, and number of vials per pouch:
Vial Types Standard Vials for Lyophilized Tests: 1.4 ml 2D barcoded tubes, internally threaded and gasketed
Other, please specify:
Documentation and Other Requirements Custom products do not include any QC unless requested by customer
CoA (Certificate of Analysis)
CoO (Certificate of Origin)
Additional Requirements, please specify:
mailto:christopher.hourigan@nih.gov mailto:christopher.hourigan@nih.gov mailto:customorders@biolegend.com mailto:infoeurope@biolegend.com mailto:christopher.hourigan@nih.gov mailto:christopher.hourigan@nih.gov mailto:customorders@biolegend.com mailto:infoeurope@biolegend.com
CST015-F5/Form-02074 Specification Form for Custom TotalSeq™ Panel Request R3 Document No. Form-02074 R3 Effective Date: 7/15/2022
C. Clones Information
Please use the “TotalSeq Panel Info-Spreadsheet.xlsx” form to describe the desired clones in the panel.
This sheet includes the following fields:
• Clone. Please enter the name of the clone.
• Specificity. Please enter the name of the target.
• Barcode. Please enter the numeric identifier of the TotalSeq Barcode associated with the clone.
• Customer specified concentration (ug antibody per 50µl test or as specified in ‘Packaging’ requirements above). If the customer has identified a working concentration for this clone in their experimental system, please enter that information here.
BioLegend Use Only
NAV Part Number 900009743 Expected Lead Time 10- 12 weeks
Approved by Approved Date
Signature for project feasibility and approving lead time
BioLegend TotalSeq Antibody Clones
TotalSeq Format
Catalog Number
(or purified cat# if not available)
Barcode (4 digits)
Specificity Clone How is ug per test/reaction determined?
(choose BioLegend default or customer specified; if customer, specify value in column H)
Customer Specified ug per lyophylized test/reaction (default reaction size is 50ul final staining volume for 500k cells) (Do not provide antibody concentration, provide absolute antibody mass to be included per antibody per reaction)
TotalSeq-C 312233 0062 CD10 HI10a Biolegend specified optimal ug/test Use 0.0625ug
TotalSeq-C 313243 0061 CD117 104D2 Biolegend specified optimal ug/test Use 0.0625ug
TotalSeq-C 301359 0161 CD11b ICRF44 Biolegend specified optimal ug/test
TotalSeq-C 371521 0053 CD11c S-HCL-3 Biolegend specified optimal ug/test
TotalSeq-C 306045 0064 CD123 6H6 Biolegend specified optimal ug/test
TotalSeq-C 301733 0364 CD13 WM15 Biolegend specified optimal ug/test
IMPORTANT NOTE: We cannot accept responsibility for any amended or additional specifications subsequent to acceptance of the order and failure to meet any such amended or additional specifications will not be accepted as cause for rejection of the product by the customer.
Ornella Salvatori (Oct 25, 2023 08:49 PDT) Ornella Salvatori
PD Manager Oct 25, 2023
CST015-F5/Form-02074 Specification Form for Custom TotalSeq™ Panel Request R3 Document No. Form-02074 R3 Effective Date: 7/15/2022
TotalSeq-C 356539 0055 CD138 MI15 Biolegend specified optimal ug/test
TotalSeq-C 301859 0081 CD14 M5E2 Biolegend specified optimal ug/test
TotalSeq-C 344125 0163 CD141 M80 Biolegend specified optimal ug/test
TotalSeq-C 302065 0083 CD16 3G8 Biolegend specified optimal ug/test
TotalSeq-C 333637 0358 CD163 GHI/61 Biolegend specified optimal ug/test
TotalSeq-C 302265 0050 CD19 HIB19 Biolegend specified optimal ug/test
TotalSeq-C 331547 0160 CD1c L161 Biolegend specified optimal ug/test
TotalSeq-C 309231 0367 CD2 TS1/8 Biolegend specified optimal ug/test
TotalSeq-C 363516 0393 CD22 S-HCL-1 Biolegend specified optimal ug/test
TotalSeq-C 302649 0085 CD25 BC96 Biolegend specified optimal ug/test
TotalSeq-C 300479 0034 CD3 UCHT1 Biolegend specified optimal ug/test
TotalSeq-C 333919 0028 CD30 BY88 Biolegend specified optimal ug/test Reference TS_D Data (0.5x)
TotalSeq-C 354241 0370 CD303 201A Biolegend specified optimal ug/test
TotalSeq-C 354527 0406 CD304 12C2 Biolegend specified optimal ug/test Reference TS_B Univ Data
TotalSeq-C 366633 0052 CD33 P67.6 Biolegend specified optimal ug/test Use 0.2ug
TotalSeq-C 343541 0054 CD34 581 Biolegend specified optimal ug/test Reference TS_D Data (0.5x)
TotalSeq-C 303543 0389 CD38 HIT2 Biolegend specified optimal ug/test
TotalSeq-C 300567 0072 CD4 RPA-T4 Biolegend specified optimal ug/test
TotalSeq-C 103063 0073 CD44 IM7 Biolegend specified optimal ug/test
TotalSeq-C 368545 0048 CD45 2D1 Biolegend specified optimal ug/test Reference 2D1 Concentration in
TBNK
TotalSeq-C 304163 0063 CD45RA HI100 Biolegend specified optimal ug/test
TotalSeq-C 304259 0087 CD45RO UCHL1 Biolegend specified optimal ug/test
TotalSeq-C 304345 0576 CD49d 9F10 Biolegend specified optimal ug/test
TotalSeq-C 300637 0138 CD5 UCHT2 Biolegend specified optimal ug/test
TotalSeq-C 362559 0047 CD56 5.1H11 Biolegend specified optimal ug/test
TotalSeq-C 304851 0147 CD62L DREG-56 Biolegend specified optimal ug/test
TotalSeq-C 304945 0218 CD62P AK4 Biolegend specified optimal ug/test
TotalSeq-C 305045 0162 CD64 10.1 Biolegend specified optimal ug/test Reference TS_B Univ Data
TotalSeq-C 310951 0146 CD69 FN50 Biolegend specified optimal ug/test
TotalSeq-C 343127 0066 CD7 CD7-6B7 Biolegend specified optimal ug/test
TotalSeq-C 334125 0394 CD71 CY1G4 Biolegend specified optimal ug/test Use 0.025ug
TotalSeq-C 344753 0046 CD8 SK1 Biolegend specified optimal ug/test
TotalSeq-C 305341 0359 CD83 HB15e Biolegend specified optimal ug/test
TotalSeq-C 328145 0060 CD90 5E10 Biolegend specified optimal ug/test Reference TS_D Data (0.5x)
TotalSeq-C 334645 0352 FceRIa AER-37
(CRA-1)
Biolegend specified optimal ug/test
TotalSeq-C 307663 0159 HLA-DR L243 Biolegend specified optimal ug/test
TotalSeq-C 394007 0594 CD133 S16016B Biolegend specified optimal ug/test No Data, use 0.25ug
CST015-F5/Form-02074 Specification Form for Custom TotalSeq™ Panel Request R3 Document No. Form-02074 R3 Effective Date: 7/15/2022
TotalSeq-C 369621 0151 CD152 BNI3 Biolegend specified optimal ug/test
TotalSeq-C 369335 0152 CD223
(LAG-3)
11C3C65 Biolegend specified optimal ug/test
TotalSeq-C 329751 0007 CD274 29E.2A3 Biolegend specified optimal ug/test
TotalSeq-C 329963 0088 CD279 EH12.2H7 Biolegend specified optimal ug/test
TotalSeq-C 345049 0169 CD366 F38-2E2 Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 305651 0156 CD95 DX2 Biolegend specified optimal ug/test
TotalSeq-C 338423 0175 CD96 NK92.39 Biolegend specified optimal ug/test
TotalSeq-C 371319 0845 CD99 3B2/TA8 Biolegend specified optimal ug/test
TotalSeq-C 353615 0853 CLEC12a 50C1 Biolegend specified optimal ug/test
TotalSeq-C 311449 0058 HLA-A,B,C W6/32 Biolegend specified optimal ug/test
TotalSeq-C 1018 HLA-
DR,DP,DQ
Tü39 Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 323131 0026 CD47 CC2C6 Biolegend specified optimal ug/test
TotalSeq-C 306533 0366 CD184 12G5 Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 303739 0353 CD41 HIP8 Biolegend specified optimal ug/test
TotalSeq-C 305523 0867 CD94 DX22 Biolegend specified optimal ug/test
TotalSeq-C 351356 0390 CD127 A019D5 Biolegend specified optimal ug/test
TotalSeq-C 313323 0351 CD135 BV10A4H2 Biolegend specified optimal ug/test Use 0.0625ug
TotalSeq-C 372729 0089 TIGIT A15153G Biolegend specified optimal ug/test
TotalSeq-C 358209 0912 GPR56 CG4 Biolegend specified optimal ug/test
TotalSeq-C 312121 0579 CD9 HI9a Biolegend specified optimal ug/test Reference TS_B Univ Data
TotalSeq-C 303225 0142 CD32 FUN-2 Biolegend specified optimal ug/test
TotalSeq-C 316021 0033 CD52 HI186 Biolegend specified optimal ug/test
TotalSeq-C 353127 0217 CD54 HA58 Biolegend specified optimal ug/test
TotalSeq-C 304715 0361 CD59 p282 (H19) Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 0234 CD68 Y1/82A Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 305447 0006 CD86 IT2.2 Biolegend specified optimal ug/test
TotalSeq-C 336123 0446 CD93 VIMD2 Biolegend specified optimal ug/test Reference TS_B Univ Data
TotalSeq-C 355705 0179 CX3CR1 K0124E1 Biolegend specified optimal ug/test
TotalSeq-C 342115 0920 CD82 ASL-24 Biolegend specified optimal ug/test
TotalSeq-C 329229 0447 CD200 OX-104 Biolegend specified optimal ug/test Reference TS_A Univ Data
TotalSeq-C 313635 0070 CD49f GoH3 Biolegend specified optimal ug/test
TotalSeq-C 350233 0145 CD103 Ber-ACT8 Biolegend specified optimal ug/test
TotalSeq-C 321147 0205 CD206 15-2 Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 337635 0023 CD155 SKII.4 Biolegend specified optimal ug/test
TotalSeq-C 302363 0100 CD20 2H7 Biolegend specified optimal ug/test
TotalSeq-C 355119 0027 CD70 113-16 Biolegend specified optimal ug/test Use 0.1ug
TotalSeq-C 400187 0090 Mouse IgG1, κ isotype Ctrl
MOPC-21 Biolegend specified optimal ug/test
CST015-F5/Form-02074 Specification Form for Custom TotalSeq™ Panel Request R3 Document No. Form-02074 R3 Effective Date: 7/15/2022
TotalSeq-C 400293 0091 Mouse IgG2a, κ isotype Ctrl
MOPC-173 Biolegend specified optimal ug/test
TotalSeq-C 400381 0092 Mouse IgG2b, κ isotype Ctrl
MPC-11 Biolegend specified optimal ug/test
TotalSeq-C 347115 0199 Siglec-8 7C9 Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 362925 1261 CD191 5F10B29 Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 320623 1136 CD181 8F1/CXCR1 Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 308613 0219 CD119 GIR-208 Biolegend specified optimal ug/test
TotalSeq-C 0578 CD79a HM47 Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 357523 0056 BCMA 19F2 Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 333021 1248 CD85k ZM4.1 Biolegend specified optimal ug/test No Data, use 0.25ug
TotalSeq-C 900009729 5078 CD151 50-6 Biolegend specified optimal ug/test Custom, use 0.25ug
TotalSeq-C 900009730 5079 CD182 5E8/CXCR2 Biolegend specified optimal ug/test Custom, use 0.25ug
TotalSeq-C 900009731 5081 CD49e NKI-SAM-1 Biolegend specified optimal ug/test Custom, use 0.25ug
TotalSeq-C 900009732 5082 MSLN MN Biolegend specified optimal ug/test Custom, use 0.25ug
TotalSeq-C 900009733 5084 CD312 W15101A Biolegend specified optimal ug/test Custom, use 0.25ug
TotalSeq-C 900009734 5085 CD97 VIM3b Biolegend specified optimal ug/test Custom, use 0.25ug
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