Attachment_1_-_Purchase_Description.pdf

Solicitation No. 75N95019Q00271 Attachment 1 – Purchase Description

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: This request is for the Neural Data Acquisition Systems. The purchase of this equipment allows more channel count for neural recording on rats, real-time biofeedback, calcium imaging and other expanded capabilities. These expanded capabilities will allow the lab to conduct 2-3 recording experiments at once without waiting months to run each additional experiment.

2. Background Information and Objective: The BNNS laboratory is interested in the neural circuits mediating associative learning and decision making and how alternations in those circuits contribute to maladaptive behaviors in neuropsychiatric disorders such as addiction. The BNNS uses rats as a model system to study behaviors and neural circuits that are believed to have direct relevance to understanding the human brain. The laboratory uses behavioral tasks based on principles derived from learning theory, combined with single unit recording, lesions, pharmacological and genetic manipulations to test hypotheses. Recording ensembles of single units from awake, behaving rats is the core competency of the lab. Each specific line item in the attached quotes have been thoroughly considered and found necessary to upgrade the lab’s single unit recording equipment.

3. Generic Name of Product: Neural Data Acquisition System

4. Purchase Description: Brand name or Equal-FAR 52.211-6 Brand Name or Equal (Aug 1999) - Omniplex components and software, manufactured by Plexon. The components of this system include the following brand name or equal items:

Line No. Item Brand Name or Equal Quantity Unit 1 Item # 94218: Neural Data Acquisition System Chassis, OmniPlex

D-DHP

1 Each

2 Item # 94220-128: 128-channel Digital Headstage Processer, Digital Headstage Processor DHP/128

1 Each

3 Item # 94219-128: OmniPlex Software, OmniPlex D SW/128 –

DHP

1 Each

4 Item # 92125-022: Digital Headstage cable to be used with

HST/64DS

2 Each

5 Item # 92410-044: 64-channel thumbtack design headstage 2 Each 6 Item # 40948: Headstage tested unit for HST/64DS Headstage 1 Each 7 Item # 91397-202: Carousel Commutator, OPT/Carousel

Commutator 2 LED-2DHST 1 Each

8 Item # 91797: Digital input cable for OmniPlex system, CBL/DI- OmniPlex-dsub25f;

1 Each

9 Item # 80000-008: Dell 6-core 5820 workstation, PLX-6 System Controller 3.7GHz

1 Each

10 Item # 33437: PlexBright optogenetic stimulation system series Y, OPT/Series Y BNC cable-100-10

1 Each

11 Item # 51382: PlexBright optogenetic stimulation system single channel, OPT/ 1ch_LED_Driver

1 Each

12 Shipping 1 Total

5. Salient characteristics: System shall be equipped with ten single unit 128 channel recording systems, with simultaneous 40kHz A/D conversion on each channel at 16 - bit resolution with 40kHz sampling rate on each channel.

• Systems shall be scalable to 512 channels, with all acquisition and processing happening in one system (i.e. cannot be multiple lower channel count systems “daisy-chained” together)

• Systems shall be equipped with software programmable digital filtering (e.g. Common Average Referencing (CAR), Common Median Referencing (CMR), channel-to-channel referencing) for wide - band, field potential, and spike frequency band isolation

• Systems shall have Real - time spike sorting in single electrode, stereotrode, or tetrode mode. Online sorting in both waveform space (time/voltage) and directly in feature space (PCA, Maxima/Minima, and others). Box, line, band, and template spike sorting in time/voltage space. 2D contour, 2D ellipse, and 3D ellipse spike sorting in feature space

• Systems shall have automatic sorting using valley - seeking and t - distribution expectation/maximization algorithms.

• Systems shall have automatic threshold setting based upon mean and standard deviation, and also “robust statistics” threshold setting (i.e. the use of the median as a robust statistic instead of the mean, or the use of the median absolute deviation (MAD) instead of the standard deviation.)

• Systems shall have Real - time spike timestamp, waveform, and digital event data link to user

- defined Matlab and C++ Clients, for customer - specific on - line analyses. Shall have Real -time Perievent histogram plots.

• Systems shall have Real - time data link with NeuroExplorer data analysis program, cross -correlation analyses, for real - time JPSTH plots, Perievent

• raster and histogram analyses, burst analyses, etc. NeuroExplorer is software package for neurophysiological data analysis for presentations.

• Systems shall have real - time interspike interval histogram display, with warning indicator for spike with interspike intervals less than user - defined refractory period.

• Shall have up to 32 digital inputs for external synch and experiment state variables from behavioral equipment, as individual TTL lines or multi - bit strobed words (see further requirements for this below).

• Shall provide lifetime Technical Support for all software and hardware

• Systems shall have 32 digital outputs for controlling third-party devices.

• Systems shall have 32 “auxiliary” analog inputs, for non-neural signal acquisition (i.e. EMG, EEG, blood pressure, speed).

• Systems shall fit within existing space on custom training rack, standard 19” rail spacing, approx. 9” high opening. Company will install the systems at our convenience, in between ongoing experiments (requiring several separate installation visits), validate their function with either artificial data or live subjects at our discretion, and validate that the systems interact with existing equipment detailed below.

• Systems shall output data in the pl2 format, which fits within our current workflow (i.e. we will not have to change any existing analysis scripts, post-processing steps, etc.).

• Systems shall be capable of receiving 15-bit words from custom lab behavioral computer to detect events, triggered via a strobed bit on the I/O card. Company shall demonstrate sub-ms resolution at event detection and detect accurately 65565 different events used in the task.

Event detection shall be validated on install and guaranteed to be backward compatible with existing behavioral programs in the lab currently, or contract will be void and equipment will be returned.

• Systems shall be capable of interfacing with and controlling PlexBright Optogenetics hardware that is currently used in the lab for experiments. This includes passing signal for recording data through the PlexBright digital commutators. Company will validate this on install or contract will be void and equipment will be returned.

• Systems shall be capable of interfacing with and controlling CineLyzer Photometry system and CinePlex tracking equipment.

• Systems shall utilize connectors at the headset and/or between the headset and the subject that are compatible with custom 16 and 32 channel electrode designs used in the lab.

Company will validate this on install or contract will be void and equipment will be returned. In addition, company will commit to develop customer 64 chan and 128 chan headstage interface.

• Systems shall be guaranteed to work with new Windows OS when current Windows 7 OS is no longer supported and sunsets, currently projected to occur in 2020.

• Company shall remove existing Plexon MAP systems from our training racks, consolidate current hardware into 32 channel systems, including the purchase of any required hardware to make this consolidation work properly.

• Proposed systems shall be perfectly compatible with our existing Plexon equipment and software. Systems need to interface with existing equipment and record 128 channels with real time event monitoring, compatibility with optogenetics equipment, and able to install in existing behavioral rigs.

• Systems shall fit within existing space on custom training rack, standard 19” rail spacing.

• Systems shall output data in the pl2 format, which fits within our current workflow.

• Systems shall be capable of interfacing with and controlling PlexBright Optogenetics hardware.

• Systems shall be capable of interfacing with and controlling CineLyzer Photometry system and CinePlex tracking equipment.

• Systems shall be guaranteed to work with new Windows OS when current Windows 7 OS is no longer supported.

• Minimum Warranty requirements shall include a three (3) year parts and labor warranty for manufacturing defects and Lifetime Technical Support.

6. Delivery Date: Up to 30-60-day lead time for delivery of data acquisition systems. Up to 100-120-day lead time for motorized commutators.

7. Deliver to:

Mia Jefferson Branch Administrative Technician Cellular Neurobiology Research Branch NIH/NIDA Intramural Research Program 251 Bayview Blvd. Suite 200 Room 06A719 Baltimore, MD 21224 443-740-2820

8. Warranty and Customer Support: Three (3) year parts and labor warranty and life-time technical support.