Purchase_Description_Bioruptor_2019.doc

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: Ultrasonicator machine and microtubes for sonication/shearing of samples. This machine will be used to sonicate/shear various biological molecules such as genomic DNA, RNA, chromatin for chromatin immunoprecipitation, cell and tissue disruption among others.

2. Background Information and Objective: The Translational Gerontology Branch (TGB) will conduct chromatin immuniprecipitation (ChIP) experiments on aged hematopoietic stem cells (HSCs). HSCs yield very low amounts of DNA which is required for ChIP. Thus, in order to maximize efficiency of these few HSCs, we require chromatin that is uniformly and efficiently sheared.

3. Generic Name of Product: Ultrasonicator

4. Purchase Description: Bioruptor Plus for 1.5 ml tubes equipped with soundproof box, water cooler, single cycle valve, and 1.5 ml tube holder, Tube Holder 0.5/0.65 ml for Bioruptor, 1.5 ml TPX microtubes for Bioruptor Plus, 0.5 ml microtubes for Bioruptor Plus

5. Salient characteristics: Bioruptor’s unique technology includes a power supply of 100-240 Vac, the control unit dimensions are 350(W) x 260(D) x 165(H) mm, sonication unit dimensions: 175(W) x 160(D) x 265(H) mm, soundproof box dimensions: 350(W) x 350(D) x 500(H) mm, and a sonication bath volume of 750 ml. It uses a digital timer, can control water flow via a connector kit for the water cooler, has an interval timer, weighs 30kG, has tube holders suitable for many sizes (0.5, 1.5, 10, 15, and 50 mL), and the various volume adaptors allow 3-12 tubes to be processed at a time based on the volume.

6. Quantity:

7. Delivery Date: Shipping should occur within 4-6 weeks of receiving order

8. Period of Performance: N/A

9. Option(s): N/A Version 11-30-2016-1

FAR Part 11—Describing Agency Needs