D_CSS_Attachment_Purchase_Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Statement of Need and Purpose: The purpose of this acquisition is to acquire one (1) Maestro APEX system, one (1) Maestro Pro Microelectrode Array Platform (MEA) platform, and accessories, or brand-name equal, for the Stem Cell Translation Laboratory (SCTL) within the National Center for Advancing

Translational Sciences (NCATS) Division of Preclinical Innovation (DPI). This brand-name or equal equipment will allow SCTL scientists to measure electrical network behavior in live cells, such as nociceptors and cardiomyocytes derived from induced pluripotent stem cells, using high-throughput technology. This equipment will also allow SCTL scientists to perform manual experiments and run multiple recordings simultaneously.

Background Information and Objective: The Maestro APEX, Maestro Pro, and accessories, or brand-name equal, are required to meet project milestones in support of the Helping to End Addiction Long

Term (HEAL) Initiative. The Maestro APEX system provides automation capabilities for all aspects of microelectrode array plate preparation, maintenance, and experiment execution. The Maestro Pro MEA

Platform allows for simultaneous live, label-free recording of electrically active cells. This will be utilized to characterize neurons, such as nociceptors and cardiomyocytes, derived from induced pluripotent stem cells. Characterization of these cells with the comprehensive Maestro system will allow SCTL scientists to gain a better understanding of the behavior of diseased cells, screen for drugs to treat that disease/disorder, and examine potential toxic effects of drug candidates.

This equipment is essential for achieving SCTL research goals to: 1) establish detailed quality control

(QC) standards to define pluripotency and differentiated cell types; 2) use multi-omics methods to assess molecular and cellular variations/signatures in cellular phenotypes derived from iPSCs; 3) develop standardized methods for producing mature cells from iPSCs that meet QC and reproducibility standards; and 4) discover, validate and disseminate small molecule reagents to replace expensive recombinant proteins, xenogenic material and undefined media components in cell differentiation protocols.

Solicitation No. 75N95019Q00139

Purchase Description: The brand name items include:

Brand Name Item Product

Number

Description Qty

Maestro APEX MAESTRO AUTO

Fully integrated robotic platform with: a 44-plate capacity automated incubator, CO2 gas mixer, ISO 14644-1 compliant HEPA filtered hood with UV, 4-channel pipettor (1-1000uL dispense), dedicated reagent racks and reservoirs, dual barcode readers, dedicated biohazard waste receptacles, and scheduling software with full AxIS feature integration.

Maestro Pro MAESTRO768PRO

768-channel MEA system with BioCore v4 chip technology, touchscreen real-time interface, stimulation, recording and data acquisition system, automated temperature and CO2 control, desktop computer, monitor, and AxIS Navigator software for acquisition and analysis. Accommodates Axion BioSystem's 12, 24, 48, and 96-well, MEA plates (SLAS compliant).

Motorized Maestro Upgrade

MOTO Upgrade In-factory service to upgrade the Maestro Pro to a motorized Maestro with automated plate docking.

Parts are included.

Installation and Training

INT-AUTO

Two-day, on-site installation and training covering hardware, software, and applications for the Maestro APEX system for existing Maestro users.

CytoView MEA 48- White plate

M768-tMEA-48W-5 Plates with 16 microelectrodes per well and white polystyrene walls with a transparent SU-8 bottom.

Box of five plates.

Performance Assurance Service Plan with Preventive Maintenance - Maestro MEA

PASPM768

OPTION: One-year, in-factory service and preventive maintenance plan including all parts and labor. Includes one in-factory preventive maintenance service.

Performance Assurance Service Plan with Preventive Maintenance - APEX System

PASPM-APEX

OPTION: One-year service and preventive maintenance plan including all parts and labor.

Includes one on-site preventive maintenance service for the automation platform and the incubator.

Salient Characteristics: The salient characteristics of the brand name or equal equipment are:

1. Must be compatible with the Axion Classic and CytoView Micro Electrode Array plates. The SCTL currently utilizes this equipment and it is imperative that the brand name or equal equipment is compatible with this existing equipment for the continuation of cell characterization.

2. Must be compatible with an existing Axion Maestro Pro.

3. The Maestro APEX or equal must include:

a. Sterile plate deck: HEPA filter and UV light package included

b. Integrated incubator: Liconic, 44-plate capacity, automated plate handling; temperature control 33-70° C; 95% max humidity

c. ECmini: Updated design providing responsive CO2 delivery; accommodates top-access to the MEA plate for the pipettor

d. Software: Complete platform operational control (robotics, incubator, AxIS) with Hamilton scheduling software; training included

e. Dimensions: dimensions must be no larger than L 74” X W 28” X H 52”

4. The Maestro Pro or equal must include:

a. 768-channel MEA system with x12 BioCore v4 chip technology

b. Automated temperature and CO2 control

c. Compatible with Axion BioSystem's 12, 24, 48, and 96-well, MEA plates (SLAS compliant)

d. Electrical stimulation of 768 microelectrodes in all wells simultaneously

e. Dimensions no larger than H 8.86” x W 11.0” x D 17.8”

Delivery Date: Delivery to be made within four (4) weeks of receipt of order by vendor. Installation and training shall be conducted on dates and times agreed upon by the vendor and the NCATS point of contact designated on the purchase order.

Period of Performance: The base 12-month period of performance for the Performance Assurance

Service Plans is approximately June 2019 – June 2020, to begin on the date of contract award.

Option(s): There are three option periods for the Performance Assurance Service Plans, with the following approximate periods:

Option Year 1: June 2020 – June 2021

Option Year 2: June 2021 – June 2022

Option Year 3: June 2022 – June 2023