D_CSS_Attachment_Purchase_Description.pdf

PURCHASE DESCRIPTION

Statement of Need and Purpose: The purpose of this acquisition is to obtain assorted proteins and small molecules manufactured by R&D Systems, Inc. These proteins and small molecules will allow NCATS scientists to understand the molecular mechanisms that regulate stem cell pluripotency and their multi-lineage differentiation capabilities, specifically focusing on defining optimum culture conditions.

Background Information and Objective: The National Center for Advancing Translational

Sciences (NCATS) requires the below list of assorted proteins and small molecules to meet project milestones in support of the Helping to End Addiction Long-term (HEAL) Initiative.

Ongoing research projects focus on understanding molecular mechanisms regulating stem cell pluripotency and their multi-lineage differentiation capabilities with a focus on defining optimum culture conditions for maintaining pluripotent stem cells and their conversion to homogeneous and mature population of differentiated cells.

The required proteins and small molecules must be manufactured by R&D Systems, Inc. It is critical that we maintain consistency with the molecules that we use in our research projects to provide uniform cell growth and viability. Purity and consistency of the molecules and proteins, as well as consistency between batches are of utmost importance to ensure consistent growth and viability of our cells. NCATS has used recombinant human proteins from R&D Systems, Inc. for the majority of cells used in our assays since its inception. Re-optimization of all existing assays would need to be performed if another manufacturer’s molecules were to be introduced, and would therefore incur tremendous costs, time, and material resources to the government.

Generic Name of Product: Assorted proteins and small molecules

Purchase Description:

# Item Catalog Number

Unit Quantity

1 Dibutyryl-cAMP, sodium salt 1141 50mg 150

2 L-Ascorbic Acid 4055 50mg 150

3 Dorsomorphin dihydrocloride 3093 50mg 10

4 A 83-01 2939 50mg 20

5 Y27632 dihydrochloride 1254 10mg 30

6 SAG 21k 5282 10mg 20

7 CHIR 99021 trihydrochloride 4953 4953 50mg 70

8 Recombinant Human/Mouse/Rat Activin A Protein 338-AC 50ug 120

9 Recombinant Human BMP-4 Protein 314-BP 50ug 120

10 Recombinant Human Oncostatin M (OSM) Protein 295-OM 50ug 140

11 LDN 193189 dihydrochloride 6053 50mg 20

12 Recombinant Human GDNF Protein, CF 212-GD/CF 50ug 100

Solicitation No. 75N95019Q00136

13 Recombinant Human beta-NGF Protein, CF 256-GF/CF 100ug 110

14 Recombinant Human NT-3 Protein, CF 267-N3/CF 25ug 60

15 SU 5402 3300 1mg 20

16 DAPT 2634 10mg 20

17 Recombinant Human Jagged 1 Fc Chimera Protein, CF 1277-JG 50ug 30

18 Recombinant Human PDGF-AA Protein, CF 221-AA 50ug 30

19 Recombinant Human DLL1 Protein, CF 1818-DL 50ug 30

20 Recombinant Human CNTF Protein 257-NT 50ug 30

21 Recombinant Human LIF Protein 7734-LF 25ug 50

22 Recombinant Human Cardiotrophin-1 (CT-1) Protein 612-CD 50ug 10

23 Recombinant Human BMP-10 Protein 2926-BP 25ug 30

24 PD 173074 3044 10mg 20

25 Sodium butyrate 3850 50mg 10

26 trans-ISRIB 5284 50mg 50

27 Recombinant Human BDNF Protein, CF 248-BDB/CF 50ug 50

Total 1500

Delivery Date: Delivery is required to be made in two (2) shipments per the following instructions:

Shipment 1: Must be delivered within 2 weeks of initial receipt of order by the vendor and must contain the following in 1 delivery:

Seventy-five (75) x Dibutyryl-cAMP, sodium salt, 50mg each

Seventy-five (75) x L-Ascorbic Acid, 50mg each

Five (5) x Dorsomorphin dihydrocloride, 50 mg each

Ten (10) x A 83-01, 50mg each

Fifteen (15) x Y27632 dihydrochloride, 10mg each

Ten (10) x SAG 21k, 10mg each

Thirty-five (35) x CHIR 99021 trihydrochloride 4953, 50mg each

Sixty (60) x Recombinant Human/Mouse/Rat Activin A Protein, 50ug each

Sixty (60) x Recombinant Human BMP-4 Protein

Seventy (70) x Recombinant Human Oncostatin M (OSM) Protein, 50ug each

Ten (10) x LDN 193189 dihydrochloride, 50mg each

Fifty (50) x Recombinant Human GDNF Protein, CF, 50ug each

Fifty-five (55) x Recombinant Human beta-NGF Protein, CF, 100ug each

Thirty (30) x Recombinant Human NT-3 Protein, CF, 25ug each

Ten (10) x SU 5402, 1mg each

Ten (10) x DAPT, 10mg each

Fifteen (15) x Recombinant Human Jagged 1 Fc Chimera Protein, CF, 50 ug each

Fifteen (15) x Recombinant Human PDGF-AA Protein, CF, 50ug each

Fifteen (15) x Recombinant Human DLL1 Protein, CF, 50ug each

Fifteen (15) x Recombinant Human CNTF Protein, 50ug each

Twenty-five (25) x Recombinant Human LIF Protein, 25ug each

Five (5) x Recombinant Human Cardiotrophin-1 (CT-1) Protein, 50ug each

Fifteen (15) x Recombinant Human BMP-10 Protein, 25ug each

Ten (10) x PD 173074, 10mg each

Five (5) x Sodium butyrate, 50mg each

Twenty-five (25) x trans-ISRIB, 50mg each

Twenty-five (25) x Recombinant Human BDNF Protein, CF, 50ug each

Shipment 2: Must be delivered 3 months after Shipment 1 is sent by the vendor and must contain the following in 1 delivery:

Seventy-five (75) x Dibutyryl-cAMP, sodium salt, 50mg each

Seventy-five (75) x L-Ascorbic Acid, 50mg each

Five (5) x Dorsomorphin dihydrocloride, 50 mg each

Ten (10) x A 83-01, 50mg each

Fifteen (15) x Y27632 dihydrochloride, 10mg each

Ten (10) x SAG 21k, 10mg each

Thirty-five (35) x CHIR 99021 trihydrochloride 4953, 50mg each

Sixty (60) x Recombinant Human/Mouse/Rat Activin A Protein, 50ug each

Sixty (60) x Recombinant Human BMP-4 Protein

Seventy (70) x Recombinant Human Oncostatin M (OSM) Protein, 50ug each

Ten (10) x LDN 193189 dihydrochloride, 50mg each

Fifty (50) x Recombinant Human GDNF Protein, CF, 50ug each

Fifty-five (55) x Recombinant Human beta-NGF Protein, CF, 100ug each

Thirty (30) x Recombinant Human NT-3 Protein, CF, 25ug each

Ten (10) x SU 5402, 1mg each

Ten (10) x DAPT, 10mg each

Fifteen (15) x Recombinant Human Jagged 1 Fc Chimera Protein, CF, 50 ug each

Fifteen (15) x Recombinant Human PDGF-AA Protein, CF, 50ug each

Fifteen (15) x Recombinant Human DLL1 Protein, CF, 50ug each

Fifteen (15) x Recombinant Human CNTF Protein, 50ug each

Twenty-five (25) x Recombinant Human LIF Protein, 25ug each

Five (5) x Recombinant Human Cardiotrophin-1 (CT-1) Protein, 50ug each

Fifteen (15) x Recombinant Human BMP-10 Protein, 25ug each

Ten (10) x PD 173074, 10mg each

Five (5) x Sodium butyrate, 50mg each

Twenty-five (25) x trans-ISRIB, 50mg each

Twenty-five (25) x Recombinant Human BDNF Protein, CF, 50ug each