36C25618U0484-0002000.docx

FedBizOpps Amendment to a Previous Combined Solicitation

CLASSIFICATION CODE

SUBJECT

CONTRACTING OFFICE'S

ZIP-CODE

SOLICITATION NUMBER

BASE NOTICE TYPE

RESPONSE DATE (MM-DD-YYYY)

ARCHIVE

DAYS AFTER THE RESPONSE DATE

RECOVERY ACT FUNDS

SET-ASIDE

NAICS CODE

CONTRACTING OFFICE

ADDRESS

POINT OF CONTACT

(POC Information Automatically Filled from User Profile Unless Entered)

DESCRIPTION

See Attachment

AGENCY'S URL

URL DESCRIPTION

AGENCY CONTACT'S EMAIL

ADDRESS

EMAIL DESCRIPTION

ADDRESS

POSTAL CODE

COUNTRY

ADDITIONAL INFORMATION

GENERAL INFORMATION

PLACE OF PERFORMANCE

* = Required Field FedBizOpps Amendment to a Previous Combined Solicitation Rev. March 2010 Project Legacy, 21 Microscopes 70112 36C25618U0484 09-26-2018 N 333314 Department of Veterans Affairs Southeast Louisiana Veterans HCS 1555 Poydras Street New Orleans LA 70112

5. PROJECT NUMBER (if applicable)

CODE

7. ADMINISTERED BY

2.

AMENDMENT/MODIFICATION NUMBER

CODE

6. ISSUED BY

8. NAME AND ADDRESS OF CONTRACTOR

4. REQUISITION/PURCHASE REQ. NUMBER

3. EFFECTIVE DATE

9A. AMENDMENT OF SOLICITATION NUMBER

9B. DATED

PAGE

OF PAGES

10A. MODIFICATION OF

CONTRACT/ORDER NUMBER

10B. DATED

BPA NO.

1. CONTRACT ID CODE

FACILITY CODE

CODE

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended, by one of the following methods:

The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offers

E. IMPORTANT:

is extended,

(a) By completing Items 8 and 15, and returning __________ copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted; or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGMENT TO BE RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY is not extended.

12. ACCOUNTING AND APPROPRIATION DATA

(REV.

11/2016) is required to sign this document and return ___________ copies to the issuing office.

is not, A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT ORDER NO. IN ITE M 10A.

15C. DATE SIGNED

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES

SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR

43.103(b).

RESULT IN REJECTION OF YOUR OFFER. If by virtue of this amendment you desire to change an offer already submitted, such change may be made by letter or electronic communication, provided each letter or electronic communication makes r eference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER

BY

Contractor

16C. DATE SIGNED

14.

DESCRIPTION OF AMENDMENT/MODIFICATION

16B. UNITED STATES OF AMERICA

Except as provided herein, all terms and conditions of the document referenced in Item 9A or 10A, as heretofore changed, remains unchanged and in full force and effect.

15A. NAME

AND TITLE OF SIGNER

16A. NAME AND TITLE OF CONTRACTING OFFICER

15B. CONTRACTOR/OFFEROR

STANDARD FORM 30

PREVIOUS EDITION NOT USABLE

Prescribed by GSA - FAR (48 CFR) 53.243 (Type or print) (Type or print) (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

(Number, street, county, State and ZIP Code) (If other than Item 6) (Specify type of modification and authority) (such as changes in paying office, appropriation date, etc.)

(If required)

(SEE ITEM 11)

(SEE ITEM 13)

(X)

CHECK

ONE

13. THIS ITEM APPLIES ONLY TO MODIFICATIONS OF CONTRACTS/ORDERS,

IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

11. THIS ITEM ONLY APPLIES TO AMENDMENTS

OF SOLICITATIONS

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

(Signature of person authorized to sign) (Signature of Contracting Officer) 629-18-3-7224-0291 Department of Veterans Affairs Southeast Louisiana Veterans HCS 1515 Poydras Street New Orleans LA 70112 Department of Veterans Affairs Southeast Louisiana Veterans HCS 1515 Poydras Street New Orleans LA 70112 To all Offerors/Bidders

36C25618U0484

X Please see attached answers to questions.

Microscope Questions and Responses objective preferences for each scope contrast preferences for each scope (phase contrast, DIC, DF, IMC, etc. for widefield and the same plus transmitted light detection for each confocal).

Laser preferences for each confocal Incubation preferences where appropriate Fluor filter choices for each system.

Camera, imaging, and analysis software preferences for each system.

Motorized choices where appropriate Illumination choices for the stereomicroscopes (transmitted, reflected, both?)

we submit the following responses for the following microscopes –

Unit #1: High Resolution Upright Microscope for Cellular Characterization (Matching existing system in the lab) Unit #2: Upright Multiphoton microscope with visible and dual line IR MP laser Unit #3: Inverted Multi-line confocal system Unit #4: 20 identical Inverted Tissue Culture microscopes Unit #5: Inverted High Content Timelapse software-integrated High Throughput Microscope Unit #6: Confocal upgrade for existing upright microscope Unit #7: 6 identical Stereo (dissecting) microscopes

Objective Preferences:

Overall Notes: Unless otherwise noted, objectives for compound microscopes (i.e., excluding stereo microscopes) must be fully compatible with all compound microscopes in the facility, including the existing Ni-E upright microscope. This allows sharing of high-value objectives rather than purchasing all objectives of interest for all microscopes. When used on the motorized inverted microscopes, these must be compatible with the focus maintenance system (see “Motorized Choices” below). The NA, working distance, and chromatic/planar correction specifications for each magnification are the minimum to meet overall project goals.

Unit #2: 25x Apochromat water dipping objective, NA 1.1, WD 2.0mm, with correction collar for use with or without coverglass. This specialty physiology objective does not need to be compatible with other microscopes, but must support the use of 1300nm light (see “Laser Preferences”). Objectives for other microscopes must be compatible with Unit #2.

Unit #4: 4x Achromat, NA 0.1, WD 30mm; 10x phase contrast achromat, NA 0.25, WD 7mm; 20x phase contrast achromat, NA 0.4, WD 3.9mm. 20 of each objective.

Contrast Preferences:

Unit #2: Widefield Fluorescence, visible scanning confocal, dual-beam multiphoton Unit #4: Phase Contrast, Brightfield Unit #6: Visible scanning confocal, fully compatible and run with the same software as the current Ni-E upright microscope.

Laser preferences

Unit #2 Unit #4: N/A

Multiphoton

1. Coherent Discovery 1300nm dual line laser

1. Autoalignment sytem: translational beam alignment, automated wavelength focal correction optics between 700-1300nm

1. Highest resonant scanning speed 720 fps 512 x 16 Direct Hardware triggering of all devices through confocal/MP controller to eliminate software overhead delays – this is critical for high speed reactions and fast moving proteins

1. Piezo Z minimum range = 450um and must be directly hardware triggered via controller to eliminate software overhead.

0. Otherwise unacceptable time delays are introduced into the system during ultra high speed events

1. Laser etched resonant mirror to control pixel clock timing – this yields an even FOV across the image; this becomes critically important during image stitchingLaser:

Permanently aligned laser system.

1. Zero alignment for the life of the system

1. All diode lasers for enhanced power, stability, and a significantly longer laser lifetime than older gas laser based solutions: such as MultiAr, HeNe, and White light lasers.

1. Diode Laser lines: 405nm, 445nm, 488nm, 514nm, 561nm, 640nm

2. Minimum power at the fiber tip >=20mW: *this is important since most companies list the power at the laser head which up to 70% can be lost by the time it gets to the fiber tip

2. 2 laser fibers (angle and flat tip single mode fibers) that share the same laser unit.

1. System needs to be able to switch between Galvo and Resonant scanning without software restart as this will be used with high speed automated cell identification and then automatically switch to ultra high resolution Galvo scanning. Simultaneous Stimulation / FRAP (not rescan)

1. Confocal detector sensitivity range: 400-850nm

1. Highest resonant scanning speed 720 fps 512 x 16

1. 25mm FOV scanning for resonant and galvo scanning.

Incubation preferences

Unit #4: N/A

Fluor filter choices

Unit #4: N/A

Camera, imaging, and analysis software:

All computer workstations must have GPU data processing capability (15x faster than CPU-based processing for analysis algorithms) Software must be same platform for acquisition, processing, analysis, deconvolution, and macro-writing. Ability to switch modes, such as widefield/confocal, point-scanning/resonance, without a software restart.

Unit #4: Color sCMOS camera, full HD (1920x1200) or better, 50fps at full resolution or better, 6µm pixels or smaller. Acquisition software with the same interface as used in all other systems, but only needs capability for capture and simple distance/area measurements.

Unit #5: Must have a CMOS camera supporting a minimum field of view of 25mm, and analysis software that can have custom pre-processing and analysis routines in a saved script to ensure all steps in analysis are repeated the same during each analysis.

Motorized choices:

Unit #3 and #5: Fully motorized nosepiece, light path changing, fluorescence turret, and stage. Nosepiece must include a focus maintenance system that is compatible with all objectives quoted, and capable of functioning with both glass and plastic specimen holders for objectives compatible usable with plastic dishes (long working distance). This system must not expose the specimen to visible light or require imaging to maintain focus, and maintain focus within 1/3 or less the focal depth of the objective. For functionality with thick specimens, the system must support focus across the following ranges above the coverslip or dish bottom:

Air objectives:

4x: +1500 μm 10x: +600 μm 20x: +300 μm 40x: +100 μm

Oil objectives:

40x: +70 μm 60x: +20 μm 100x: +15 μm

The system should be constantly monitoring for focus changes, without software assistance from the PC, and be able to react to any focal change within the specified ranges within 30ms and correct the focus within 700ms from the maximum displacement supported by the objective.

See attached document: Microscope Questions and Responses.