21-002829 - Purchase Description_031821.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The Human Genetics Branch, Section on

Developmental Neurogenomics (SDN) studies the molecular and cellular bases of atypical brain development in neurogenetic disorders that increase risk for psychiatric illness. The SDN wet lab collects biological materials including blood, saliva and skin cells from study participants with genetically-defined neurodevelopmental disorders.

Primary tissues are reprogrammed into induced Pluripotent Stem Cells (iPSCs), and subsequently in in vitro models of central nervous system cell types. Upgrading SDN’s lab with a state-of-the-art microscope, will allow it to pursue these, and higher cell modeling studies such as 3-D brain organoids generated from iPSCs. These in vitro 3D structures will enable SDN to better recapitulate and study the structure, development and function of the human brain. Fundemental to SDN’s research program is its ability to culture, maintain and image our cells and organoids. This requires a high quality, motorized microscope with a large field of view, both dedicated phase contrast and coverslip corrected lenses, brightfield and fluorescence modules, and dedicated color and monochrome cameras. This microscope will be used both for live imaging and also for imaging fixed, stained cells,therefore, it is required to have the system capable of automated imaging, able to do both acquisition and quatitative analysis of fluorescence imaging on the same software interface. This is essential for the work in the lab, and this will allow SDN to create publication quality images. In addition, SDN is in need of an stereomicroscope for routine checking of biological materials as they are prepared for imaging on the inverted microscope system.

2. Background Information and Objective: It is essential when doing any cell culturing procedure to visually inspect microscopic cells grown in culture. This allows for evaluation of the health of the cells, the presence or absence of contamination and the levels of confluency. This is also true for work including organoid culture, where it is critical to be able to monitor the differentiation status of the cells. This is done both visually and with fluorescent biological markers. In addition to making visual examinations of the cells, it is essential to be able to document with high resolution and image quality the condition of the cells for publication. It is essential to SDN’s research program that it acquire an optically superior microscope system and outfit it with a high quality scientific cmos camera for capturing images. For full functionality, SDN requires motorization for both phase and fluorescent imaging to acquire multichannel images and to collect multiple Z dimensions. Motorization of Z will also allow SDN to do a full range of autofocus routines. Further, motoriziation will allow SDN to automate imaging in multi-well imaging vessels and stitching and tiling applications.

3. Generic Name of Product: Motorized, inverted microscope system with 25mm field of view, fluorescence, imaging and artificial intelligence capabilities plus stereomicroscope and camera.

4. Purchase Description: The purpose of this requirement is to obtain a motorized, inverted microscope with 25 mm field of view; and a stereomicroscope for routine evaluation.

5. Salient characteristics

The salient characteristics of the motorized inverted microscope system include:

1. An inverted microscope platform

2. Motorized features, including:

a. motorized Z Cam-link motor,

b. motorized DIC sextuple nosepiece

c. motorized 7 position condenser turret,

d. motorized 6 position epi-fluorescent turret,

e. motorized port selector, and

f. intelligent 1.5X tube lens

3. LED light sources for transmitted diascopic and episcopic light including a motorized shutter

4. The LED epifluorescent light source must be a white light source and include an internal shutter, which can be controlled in software.

5. The field of view (FOV) of the inverted microscope to the side camera ports must be 25 mm. Fly-eye lenses must be built into the light path to provide uniform illumination from edge to edge.

6. Fluorescent cubes must be 32 mm to accommodate the large field of view of the inverted microscope.

7. The microscope must include the necessary optics to do phase contrast. This includes 4X, and 10X Plan Fluor objectives with both Dark Low (DL) phase contrast. And 20X and 40X Plan Fluor

ELWD ADM phase objectives.

8. Additionally, two dedicated fluorescence lenses, a Plan Apo 20X

0.75 NA WD 0.66 mm and Plan Fluor 40X oil 1.3 NA WD 0.2 mm

objectives for dedicated fluorescent imaging must be included.

9. The system must come with a high quality camera able to run triggered acquisitions.

10. The system must run in software that includes both image acquisition and analysis modules, including but not limited to image stitching, multichannel acquisition,multiposition, time lapse, measurement, counting, intensity measurement, denoise.ai

(artificial intelligence module) and full documentation for scale bars and annotation of the images.

11. The software must include Extended Depth of Focus module for making Z volume images with cameras and include JOBS scripting interface for making custom workflow and acquisition/analysis routines.

12. A PC is to be included with this system that is 64 GB DDR4 RAM and 12 TB data storage. A 27” LCD monitor must be included as well.

13. The scope must be upgradable to laser based applications, such as point scanning confocal, spinning disk confocal, TIRF, or additional camera based detection.

A stereomicroscope with parallel optics must include:

14. A 8:1 zoom range, 10X-80X mag

15. A 1X Plan Achro lens, 78mm WD, 0.029 NA.

16. Both transmitted and reflected light

17. The flexibility of being upgraded to fluorescence and alternate diascopic lighting solutions

18. A 2MP color cMOS camera with 1920x1920 pixels; 30fps

19. A camera which takes images independent of a PC, or be coupled with a PC or tablet.

6. Quantity: One inverted microscope and one stereomicroscope

7. Delivery Date: The product will be delivered no later than 60 days after receipt of the

PO. All labor, materials, and shipping are to be provided by the contractor.

8. Training: Following delivery and installation, the contractor shall provide no less than 8 hours of on-site training for the routine operation of the inverted research microscope.

9. Warranty: A minimum of (1) at least a 12-month comprehensive warranty, and (2) four years for optical and mechanical is required.

10. Option(s): N/A