2023_QAL_LADTS PWS RFI SS DRAFT 21 AUG 2023.pdf

NIEHS - Laboratory Animal Diagnostic Testing Services Performance Work Statement

1.0 BACKGROUND INFORMATION

The Comparative Medicine Branch (CMB) at the National Institute of Environmental Health Sciences (NIEHS) is responsible for the health and well-being of the research animal population. Laboratory animals are susceptible to many infectious diseases which may be symptomatic or subclinical but still have profound effects on research. Some of these diseases can be zoonotic (contagious to humans). The identification of pathogenic agents infecting animals at NIEHS is important to maintain animal health and well-being, the quality of research, and the safety of personnel. The Quality Assurance Laboratory (QAL) within CMB is responsible for the NIEHS animal health surveillance program.

Sentinel animals and environmental testing are used most often to monitor for pathogenic agents. This monitoring may include a complete sentinel necropsy, physical examination for ecto- and endoparasites, and the testing of animal tissues or fluids. Environmental testing of animal housing swabs or filter media is also performed. The QAL is responsible for reporting the health testing data to the appropriate veterinarian(s) and researchers. The primary scope of the contract will be to perform diagnostic testing services as part of the animal health surveillance program. Identification of unknown bacterial or fungal isolates using Matrix Assisted Laser Desorption/Ionization – Time of Flight (MALDI-TOF) spectrophotometry or partial genomic sequencing of biological samples may also be requested. Other assays, such as Western Blot or Immunofluorescent Antibody (IFA) may be requested to confirm initial, equivocal, serological results. The purpose of this acquisition is to obtain the services of a commercial laboratory to conduct the diagnostic testing on rodent blood/sera, rodent tissues, rodent fecal specimens, biological samples (e.g., cell cultures, viral vectors, isolated proteins, etc.), and environmental swabs or filter media.

2.0 OBJECTIVES

a. The purpose of the proposed contract is to provide a readily available source for diagnostic testing services as a part of the QAL overall animal diagnostic and health monitoring programs at NIEHS.

b. The specific objective is to have a Contractor receive the biological samples, perform requested testing on these samples and report all results to the Contracting Officer Representative (COR) via e-mail, telephone, or other electronic source (e.g., online access).

3.0 SERVICES TO BE PERFORMED

I. General Requirements

a. The Contractor’s lab shall be fully equipped to provide state-of-the-art serological testing, PCR assays, and partial genomic sequencing prior to bidding on this contract. MALDI-TOF spectrophotometry capabilities for microbial identification are preferred but not required. Appropriate assay positive and negative controls should be routinely utilized for all assays. All equipment used in the testing procedures shall be maintained in good working condition. Calibration of all devices and testing equipment shall be done with sufficient frequency as to ensure accurate results. No additional instrumentation shall be purchased with the contract funds.

b. The Contractor shall be capable of performing diagnostic testing services for all agents listed in the SERVICES LIST in accordance with standard laboratory practices and state of the art microbiological procedures. The number of tests requested per sample will depend upon the nature of the individual sample.

c. The Contractor shall provide all necessary submission forms and instructions for preparation and packaging of biological samples submitted for testing.

d. The Contractor will provide specialized sample collection materials such as blood spot collection cards and flocked/sticky swabs at no charge to the Government.

e. The cost of shipping samples from the Government to the Contractor will be at the Contractor’s expense. The shipping container(s) will be delivered to the Contractor by express overnight delivery service

f. Upon receipt of the samples, the Contractor shall log all sample information into a database at the Contractor’s laboratory. The Contractor shall provide the Government with a web-based portal to confirm sample receipt.

g. Results of all testing shall be recorded in a database at the Contractor’s Laboratory within the deadlines outlined below. The Contractor shall provide the Government with a web-based portal to access results.

h. The Contractor shall also provide results in an electronic form to be delivered by email to the COR within the deadlines outlined below.

i. The Contractor will immediately notify the COR if sample results are delayed beyond the stated reporting timelines and provide an estimated completion time. Extensions to reporting requirements may only be granted by written consent (email acceptable) of the

COR.

j. The COR may also request that the questionable sample(s) be sent at the Contractor’s expense to another laboratory specified by the Government for confirmatory testing.

k. In the event of a pathogen outbreak affecting the NIEHS research animal population, the Contractor shall be able to perform or assist in research to help elucidate the cause, pathogenesis, and epidemiology of the causative agent as requested by the COR.

l. After completion of the serological, PCR or genomic sequencing, the test samples (if sufficient quantity remains) shall be stored for six (6) months at -70°C. During this period, stored samples shall be returned to the COR (or authorized designee) if so requested.

After six months, the samples may be discarded.

m. All work under this contract shall be monitored by the COR whose position is defined elsewhere in the contract.

II. Specific Requirements for Serological Testing

a. The Contractor shall be prepared to perform serological assays by appropriate methods such as Multiplex Fluorescent Immunoassay

(MFIA), Immunofluorescent Antibody (IFA), Enzyme-Linked Immunosorbent Assay (ELISA), or Hemagglutination Inhibition (HAI), as appropriate on all agents listed in the SERVICES LIST – “Individual Serological Assay”. IFA and/or Western Blot methodology should also be available for confirmatory testing.

b. The Contractor will clearly list in their proposal the primary method of testing used and any serological assays they are unable to perform in their own laboratory.

c. If serological assays for new or emerging pathogens are made available by the Contractor during the contract period, testing for these agents can be performed under this contract and listed on reports and invoices under “Individual Serological Assay – (agent name)” and billed at the same cost as all other individual serological assays.

d. Results of all serological tests shall be reported within 3 working days after receipt of the samples by the Contractor.

e. In the event of a pathogen outbreak, the COR may request expedited processing of specimens for serological testing deemed critical by the COR. The COR will notify the Contractor regarding sample number and tests required prior to shipment. These samples shall be processed by the Contractor and resulting data transmitted to the COR within 1 working day.

III. Specific Requirements for Polymerase Chain Reaction (PCR) Assays

a. The Contractor shall be prepared to perform PCR assays for all agents listed in the SERVICES LIST – “Individual PCR

Assays”.

b. The Contractor will specify in their proposal if a particular assay is performed using traditional methods including gel electrophoresis for product visualization or fluorescent detection methods such as quantitative, real-time PCR (qRT-PCR).

c. The Contractor will clearly list in their proposal any PCR assays they are unable to perform in their own laboratory.

d. The Contractor will provide in their proposal the validated limit of detection (LOD) or limit of quantification (LOQ) for each agent tested.

e. If PCR assays for new or emerging pathogens are made available by the Contractor during the contract period, testing for these agents can be performed under this contract and listed on reports and invoices under “Individual PCR Assay – (agent name)” and billed at the same cost as all other individual PCR assays.

f. Results of routine PCR tests shall be reported to the COR within 5 working days after receipt of samples by the Contractor.

g. In the event of a pathogen outbreak, the COR may request expedited processing of specimens for PCR deemed critical by the COR.

The COR will notify the Contractor regarding sample number and tests required prior to shipment. These samples shall be processed by the Contractor and resulting data transmitted to the COR within 3 working days.

IV. Specific Requirements for Matrix Assisted Laser Desorption/Ionization – Time of Flight (MALDI-TOF) Spectrophotometry

a. The Contractor shall clearly state in their proposal if they are capable of microbial identification using MALDI-TOF spectrophotometry to identify bacterial or fungal agents submitted by the Government.

b. If the Contractor is unable to identify the bacterial or fungal agent at the Genus level via MALDI-TOF spectrophotometry, the

Contractor should be capable of performing partial genomic sequencing as described in the following section, “5. Specific Requirements for Partial Genomic Sequencing” for the purpose of identification at no additional charge.

c. Results of MALDI-TOF spectrophotometry shall be reported to the COR within 5 working days after receipt of samples by the Contractor.

V. Requirements for Partial Genomic Sequencing

a. The Contractor shall be prepared to perform partial genomic sequencing analysis of standard bacterial gene targets such as 16s rRNA, rpoB, gyrA, or gyrB on submitted bacterial samples and universal fungal gene targets such as 18s rRNA or ITS for the purpose of organism identification.

b. Results of partial genomic sequencing analysis shall be reported within 15 working days after receipt of samples by the Contractor.

The results will include a copy of the generated sequence and the database(s) used for sequence analysis.

VI. Training

a. The Contractor shall provide a training program for employees involved with the contract. A copy of the training program shall be provided in the proposal and furnished to the COR when changes occur.

VII. Site Visit

a. The COR and/or an authorized representative may make an annual visit to the Contractor’s facility at the Government’s expense to inspect and review all procedures covered in the contract. The COR shall tour the facility with a representative of the Contractor. The COR may observe all procedures mentioned in the contract while visiting the Contractor’s facility.

4. DELIVERABLES

In addition to the required testing results reports set forth elsewhere in the contract, the contractor will provide yearly, and final technical reports as described below. These reports will require descriptive information about the activities undertaken during the reporting period and will require information about planned activities for future reporting periods. Electronic copies of these reports (e.g., PDF) sent to the COR via email are acceptable. The final report will be signed by the Project Supervisor.

A. Yearly Report

The Contractor shall provide the COR with a detailed description of all services provided during the previous contract year (base and all options) and will be due within 30 days of the end of the contract year. At a minimum, these tabulated reports will include the following information.

Service Provided Total Quantity Total Costs Billed Individual Serological Assay – (Agent Name) Individual PCR Assay – (Agent Name) Mouse Serology Panel 1, 2 or 3 Rat Serology Panel 1 or 2 NIEHS Mouse Environmental/Fecal PCR Panel NIEHS Rat Environmental PCR Panel Biological PCR Panel 1 Biological PCR Panel 2 MALDI-TOF Microbial ID Sequence Analysis for Microbial ID

Total Costs

• Individual Serological and PCR Assays will be listed as separate line items for each agent tested.

• Only specific services performed will be listed.

B. Final Report

A formal, comprehensive, final report, that can be understood by non-technical personnel shall be submitted within 30 days of the end of the contract to the COR. The report shall include a summation of the results furnished to the Comparative Medicine Branch (CMB), National Institute of Environmental Health Sciences (NIEHS). The report shall include any problems in testing that were incurred during the contract, the number of positive samples detected for each agent. This report shall include all necessary tables, charts, drawings and data sheets. The report shall include, at a minimum:

a. Preface: Brief Introduction

b. Acknowledgements: Name, title, and responsibilities of each key staff member on the project; of Subcontractors and of consultants; as well as recognition of committee members; etc.

c. Project Summary: The text should include, but need not be limited to:

• The purpose of the project

• Background including discussion of issues and pertinent historical facts and other information essential to full understanding of the project

• Methodology designed and used, and its execution, including any major difficulties and their resolution

• Appropriate other aspects of the total contract efforts

• A summary of the salient results achieved during the performance period

• Conclusions

d. Recommendations, with supporting rationale, as appropriate to the Government and/or the private sector

e. An assessment of the degree of success in achieving the goals of the project.

f. As it relates to Green requirements, the Contractor will include details on their use of green purchasing in their task, process, packaging, marking, delivery, training, and environmentally preferable products

g. Appendices (if applicable)

5. DELIVERIES OR PERFORMANCE

I. Deliveries

a. Satisfactory performance of the contract shall be deemed to occur upon delivery and acceptance by the Contracting Officer (CO), and/or the Contracting Officer Representative (COR) of the services described in the contract in accordance with all the terms and conditions of the contract, including the stated delivery schedule.

b. Deliveries required by the Contractor shall be made f.o.b. destination as set forth in FAR 52.247-35, F.O.B. Destination, Within Consignees Premises (April 1984) and any specifications stated in “Packaging, Marking and Shipping” Section of the contract.

c. Unless otherwise specified, deliveries shall be made to the Delivery Point specified above Monday through Friday (excluding Federal Holidays) between the hours of 8:30 a.m. and 5:30 p.m. EST only. Supplies or services scheduled for delivery on a Federal holiday shall be made the following workday.

II. Delivery shall be to the locations as specified throughout the contract and shall be made at no additional cost to the Government. No additional cost will be made to the Government for travel unless specifically authorized otherwise in the contract.

III. Deliverables

Item No.

Description Deliver To Schedule

1 Completed Test Results COR • Completed within days of sample receipt

• Electronic results are acceptable o Serology – 3 days o PCR – 5 days o MALDI-TOF – 5 days o Genomic Sequencing – 15 days

3 Annual Reports COR Annually (Electronically)

4 Final Report COR & CO End of the Contract period (Electronically)

5 Commercial Product Catalog CO Annually (Electronically)

6 A List of Supervisory Personnel authorized to act on behalf of the Contractor with Contact Information

COR & CO Contract start or as changes occur (Electronically)

7 Training Program COR & CO With proposal or as changes occur (Electronically)

IV. Deliverables shall be in accordance with the SOW/contract to the following individuals unless otherwise stated:

a. National Institute of Environmental Health Sciences (NIEHS) Attn: COR TBD Research Triangle Park (RTP), NC 27709

b. NIEHS

OM, OA, Contracts Branch C Attn: TBD (CO) 530 Davis Drive

1st Floor, 1049 Morrisville, NC 27560

V. Green Requirements shall apply if applicable to Deliveries of supplies and/or services, such as:

a. Services o Require electronic deliverables o Limit number of hard copies o Favor black/white, double-sided, soy-based ink

6. KEY PERSONNEL

I. Project Supervisor - This individual shall be responsible for overseeing all aspects of this contract including serological and PCR testing, MALDI-TOF spectrophotometry, genomic sequence analysis, reporting and contract personnel.

a. Educational requirements: This individual shall have a Ph.D. in microbiology, or DVM/VMD from an accredited school of veterinary medicine and be board certified by the American College of Laboratory Animal Medicine (ACLAM).

b. Experience: This individual shall have a minimum of five (5) years in laboratory rodent serology testing, PCR assays, MALDI-

TOF spectrophotometry, and genomic sequencing.

II. Serology Laboratory Supervisor - This individual is responsible for supervision of personnel performing serological assays and will serve as the principle contact with the COR for serological assays.

a. Educational requirement: This individual shall have a minimum of a bachelor’s degree.

b. Experience: This individual shall have a minimum of two (2) years supervisory experience in laboratory serology testing.

III. PCR Laboratory Supervisor. This individual is responsible for supervision of personnel performing PCR assays and partial genomic sequence analysis and will serve as the principle contact with the COR for PCR assays and sequencing.

b. Experience: This individual shall have a minimum of two (2) years supervisory experience in laboratory PCR testing.

IV. Spectrophotometry Laboratory Supervisor. This individual is responsible for supervision of personnel performing MALDI-TOF spectrophotometry testing and will serve as the principle contact with the COR for this testing.

b. Experience: This individual shall have a minimum of two (2) years supervisory experience in MALDI-TOF spectrophotometry.

V. Laboratory Technicians. These individuals shall be responsible for performing all testing.

a. Educational requirement: Individuals must be a high school graduate and have completed a minimum of one year of college or comparable technical training.

b. Experience: These individuals must have a minimum of six months experience performing serologic or PCR assays.

VI. Billing Specialist. This individual shall be responsible for processing and transmittal of invoices to the COR for approval.

a. Educational requirements: This individual must be a high school graduate.

b. Experience: This individual shall have 6 months experience in word processing and spreadsheet or optional billing invoice preparation software.

7. COMPLIANCE (508 Compliance (36 CFR Part 1194 - Information and Communication Technology Standards and Guidelines Compliance)):

If deliverables includes features and functions in addition to those identified as requirements, these features and functions also need to conform to relevant Section 508 technical provisions, functional performance criteria, and information, documentation and support.

a) Section 508 Information: http://www.section508.gov/

b) Guide to the Section 508 Standards: https://www.access-board.gov/guidelines-and- standards/communications-and-it/about-the-section-508-standards/guide-to-the-section-508-standards

c) Making Files Accessible: http://www.hhs.gov/web/section-508/making-files-accessible/index.html

NOTE: If the deliverable includes features and functions in addition to those identified as requirements, these features and functions also need to conform to relevant Section 508 technical provisions, functional performance criteria, and information, documentation, and support.

8. QUALITY CONTROL: The Government is committed to a highly interactive relationship between quality control by the contractor and quality assurance by the government. This relationship shall be achieved through an effective prevention based quality control program dedicated to ensuring the best possible products and services to end users. As part of quality control, the Contractor shall submit a Quality Control Plan (QCP) which at a minimum shall include a self-inspection plan, an internal staffing plan, and an outline of the procedures that the contractor shall use to maintain quality, timeliness, responsiveness, customer satisfaction, and any other requirements set forth in this SOW. The QCP shall be updated five (5) days after contract award. The QCP shall document how the contractor intends on meeting and complying with the quality standards established in this SOW. The contractor’s QCP shall be maintained throughout the life of the contract and shall include the contractor’s procedures to routinely evaluate the effectiveness of the plan to ensure the contractor is meeting the performance standards and requirements of the contract.

9. GOVERNMENT FURNISHED PROPERTY, EQUIPMENT, AND SERVICES: This section identifiesthose items such as property, information and/or services that will be provided for the contractor’s use (without cost to the contractor): N/A http://www.section508.gov/ http://www.access-board.gov/guidelines-and-http://www.hhs.gov/web/section-508/making-files-accessible/index.html

10. CONTRACTOR FURNISHED ITEMS AND RESPONSIBILITIES: The Contractor shall furnish all supplies, equipment, and services required to perform work under this contract that are not listed under section 9 of this SOW.

• Training: The Contractor shall provide a training program for employees involved with the contract. A copy of the training program shall be furnished to the COR.

• Facilities & Equipment: The Contractor’s lab shall be fully equipped (laboratory and computer equipment) to perform serological testing and PCR assays prior to bidding on this contract. Equipment used in these testing procedures shall be maintained in good working order. Calibration of all devices and testing equipment shall be done with sufficient frequency as to ensure accurate results.

11. HOURS OF OPERATION: Normal business hours are Monday-Friday, excluding Federal holidays and core hours at NIEHS typically fall between:

8:00 am – 5:00 pm.

12. PLACE OF PERFORMANCE: Each delivery order will specify the specific delivery location.

13. PERIOD OF PERFORMANCE: The contract has a five (5) year ordering period. Individual task orders issued cannot exceed a 12-month period.

14. SAFETY AND HEALTH: The Contractor shall comply with all applicable USNRC, Federal, North Carolina State, Occupational Safety and Health

Administration (OSHA) standards and local laws and regulations concerning the environmental, occupational safety and health, and hazardous material. The contractor shall ensure employees have all required training and certifications related to OSHA requirements. The documentation shall be readily available for the Contracting Officer to review at any time during the life of the contract.

15. DATA RIGHTS: The Government has unlimited rights to all documents/material produced under this contract to the extent permitted by the data rights clauses. The parties mutually acknowledge their understanding that this is the government’s intent. All documents and materials produced under this task order shall be government owned and are the property of the government with all rights and privileges of ownership/copyright belonging exclusively to the government. These documents and materials may not be used or sold by the contractor without written permission from the contracting officer. All materials supplied to the Government will be the sole property of the government and may not be used for any other purpose. This right does not abrogate any other government rights.

ATTACHMENT I Performance Requirements Summary

Task Deliverable Performance Indicator

Acceptable Quality Level

(AQL)

Monitoring Method

Maximum Payment % for Meeting or Exceeding the AQL (Incentives)

Serological Testing

Tests results on submitted samples

% processed and delivered by specified times

100% testing on rodent blood or sera

Contracting Officer Representative (COR) oversight and feedback from end user

100% payment in meeting Government requirements for testing in accordance with standard laboratory practices

PCR Testing Test results on submitted samples

% processed and delivered by specified times

100% testing on PCR Assays and Identification of Agents

COR oversight and feedback from end user

100% payment in meeting Government requirements for testing in accordance with standard laboratory practices

MALDI-TOF

Spectrophotometry

Test results on submitted samples

% processed and delivered by specified times

100% testing on MALDI-TOF and Identification of Agents

COR oversight and feedback from end user

100% payment in meeting Government requirements for testing in accordance with standard laboratory practices

Genomic Sequence Identification of Agents

Test results on submitted samples

% processed and delivered by specified times

100% testing on genomic sequencing and Identification of Agents

COR oversight and feedback from end user

100% payment in meeting Government requirements for testing in accordance with standard laboratory practices

Timeliness in Delivery of Testing Results

Telephonic, electronic and/or written tests results

% Samples items tested and delivered as requested

100% items tested and delivered

COR oversight, review, and acceptance – only minor edits required

100% payment for meeting requirement. Non-delivery is unacceptable

Timeliness in Delivery of Written Reports, Yearly & Final)

Yearly Report

Final Report

% accuracy of written report

100% accuracy of final written report

COR’s oversight, review and acceptance

100% payment for meeting requirement. Non-delivery is unacceptable

ATTACHMENT II

National Institute of Environmental Health Sciences

Laboratory Animal Diagnostic Services

SERVICE LIST

INDIVIDUAL SEROLOGICAL ASSAY AGENT ABBREVIATION SPECIES

Clostridium piliforme TYZ M, R Ectromelia Virus ECTR M Encephalitozoon cuniculi ECUN M, R Filobacterium rodentium CARB M, R Hantaan Virus HANT M, R Infectious Diarrhea of Infant Rats IDIR R Kilham Rat Virus KRV R Lactate Dehydrogenase-Elevating Virus LDEV (LDV) M Lymphocytic Choriomeningitis Virus LCMV M, R Minute Virus of Mice MVM (MMV) M Mouse Adenovirus - Strain 1 MAD1 (FL) M Mouse Adenovirus - Strain 2 MAD2 (K87) M Mouse Cytomegalovirus MCMV M Mouse Hepatitis Virus MHV M Mouse Kidney Parvovirus MKPV M Mouse Norovirus MNV M Mouse Parvovirus MPV (1-5) M Mouse Rotavirus (Enzootic Diarrhea of Infant Mice) MRV/EDIM M Mouse Thymic Virus MTV (MTLV) M Mycoplasma arthritidis M arthriditis M, R Mycoplasma pulmonis M pulmonis M, R Parvovirus NS-1 Protein NS-1 (Parvo) M, R Pneumocystis carinii P carinii R Pneumonia Virus of Mice PVM M, R Pneumonitis Virus K Virus M Polyoma Virus POL M Rat Coronavirus RCA/SDAV R Rat Cytomegalovirus RCMV R Rat Minute Virus RMV R Rat Parvovirus RPV R Rat Polyomavirus 2 RPyV2 R Rat Theilovirus RTV R Reovirus (Type 3) REO3 M, R Sendai Virus SEN M, R Theiler's Encephalomyelitis Virus TMEV or GDVII M Toolan's H-1 Virus H1 R

ATTACHMENT III

SERVICES LIST

SEROLOGICAL PANELS SPECIES AGENTS INCLUDED IN PANELS

Mouse Serology Panel 1 M

EDIM

MHV

MNV

MPV (1-5)

MVM

NS-1 (Parvo)

Mouse Serology Panel 2 M

EDIM

MHV

MNV

MPV (1-5)

MVM

NS-1 (Parvo)

M pulmonis

PVM

REO3

SEND

TMEV

Mouse Serology Panel 3 M

EDIM

MHV

MNV

MPV (1-5)

MVM

NS-1 (Parvo)

M pulmonis

PVM

REO3

SEND

TMEV

CARB

ECTR

ECUN

HANT

K Virus

LCMV

LDEV

MAD1

MAD2

MCMV

MTV

POL

Rat Serology Panel 1 R

H-1

KRV

M pulmonis NS-1 (Parvo)

PVM

RCV/SDAV

REO3

RMV

RPV

RTV

Rat Serology Panel 2 R

H-1

KRV

M pulmonis NS-1 (Parvo)

PVM

RCV/SDAV

REO3

RMV

RPV

RTV

SEND

CARB

ECUN

HANT

LCMV

P. carinii

ATTACHMENT IV

INDIVIDUAL POLYMERASE CHAIN REACTION (PCR) ASSAY AGENT ABBREVIATION

Bordetella bronchiseptica B. bronchiseptica Bordetella hinzii/pseudohinzii B. hinzii/pseudohinzii Campylobacter spp. Campylobacter spp.

Chilomastix spp. Chilomastix spp.

Citrobacter rodentium C. rodentium Clostridium piliforme C. piliforme Clostridium perfringens C. perfringens Corynebacterium bovis C. bovis Corynebacterium kutscheri C. kutscheri Cryptosporidium spp. Cryptosporidium spp.

Ectromelia Virus ECTR Encephalitozoon cuniculi ECUN Entamoeba muris E. muris Filobacterium rodentium (CAR Bacillus) CARB Hantaan Virus HANT Helicobater spp. (speciation included) HELICO Infectious Diarrhea of Infant Rats IDIR Kilham Rat Virus KRV Klebsiella oxytoca K. oxytoca Klebsiella pneumoniae K. pneumoniae Lactate Dehydrogenase-Elevating Virus LDEV (LDV) Lymphocytic Choriomeningitis Virus LCM/LCMV Minute Virus of Mice MVM (MMV) Mouse Adenovirus - Strain 1 & 2 MAD (FL & K87) Mouse Astrovirus ASTRV Mouse Cytomegalovirus MCMV Mouse Hepatitis Virus MHV Mouse Norovirus MNV Mouse Parvovirus MPV Mouse Rotavirus (Enzootic Diarrhea of Infant Mice) MRV/EDIM Mouse Thymic Virus MTV (MTLV) Mycoplasma arthritidis M arthriditis Mycoplasma pulmonis M pulmonis Mycoplasma spp. Mycoplasma spp.

Pneumocystis carinii P. carinii Pneumocystis muris P. muris

Pneumonia Virus of Mice PVM

INDIVIDUAL POLYMERASE CHAIN REACTION (PCR) ASSAY AGENT ABBREVIATION

Pneumonitis Virus K Virus Pseudomonas aeruginosa P. aeruginosa Polyoma Virus POL Rat Coronavirus RCA/SDAV Rat Minute Virus RMV Rat Parvovirus RPV Rat Polyomavirus 2 RPyV2 Rat Theilovirus RTV Reovirus (Types 1-4) REO Rodent Fur Mite (speciation included) Rodent Fur Mite Rodentibacter pneumotropicus R. pneumotropicus Rodentibacter heylii R. heylii Rodent Pinworm (speciation included) Rodent Pinworm Salmonella spp. Salmonella spp.

Segmented Filamentous Bacterium (SFB) SFB Sendai Virus SEND Spironucleus muris S. muris Staphylococcus aureus S. aureus Streptococcus pneumoniae S. pneumoniae Theiler's Encephalomyelitis Virus TMEV or GDVII Toolan's H-1 Virus H1

ATTACHMENT V

GROUP POLYMERASE CHAIN

REACTION (PCR) ASSAYS AGENTS INCLUDED IN PANELS

Biological PCR Panel - 1

ECTR

EDIM

HANT

K virus

LCMV

LDEV

MAD (FL & K87)

MCMV

MHV

MNV

MPV(1-5)

MTV

MVM

Mycoplasma spp.

M. pulmonis

POL

PVM

REO3

SEND

TMEV

Biological PCR Panel 2

LDEV

MHV

MNV

MPV(1-5)

MVM

Mycoplasma spp.

REO3

TMEV

NIEHS Mouse Environmental/Fecal PCR Panel

HELICO

MHV

MNV

MPV

MVM

TMEV

Rodent Pinworms Rodentibacter pneumotropicus/heylii

NIEHS Rat Environmental PCR Panel

RPV

RMV

KRV

H1

RTV

HELICO

Rodent pinworms Rodent fur mites Rodentibacter pneumotropicus/heylii

NIEHS Prevalent Environmental PCR Panel

HELICO

Rodent pinworms Rodent fur mites Rodentibacter pneumotropicus/heylii

Other Specialized Service MALDI-TOF Microbial Identification Sequence analysis for microbial Identification

ATTACHMENT VI

Contractor Pricing Sheet

Test

Estimated Maximum Number per Year

Unit Price

Unit Base Year Year 1 Year 2 Year 3 Year 4 Year 5 Individual Serology Assay Single assay 200 Serological Panels

Mouse Panel 1 Single panel 1500 Mouse Panel 2 Single panel 700 Mouse Panel 3 Single panel 450 Rat Panel 1 Single panel 15 Rat Panel 2 Single panel 20

Individual PCR Assay Single assay 1500 PCR Panels

Biological PCR Panel 1 Single panel 150 Biological PCR Panel 2 Single panel 100 NIEHS Mouse Environmental/Fecal PCR Panel Single panel 350 NIEHS Rat Environmental PCR Panel Single panel 15 NIEHS Prevalent PCR Panel Single Panel 50

MALDI-TOF Microbial Identification Single assay 100 Sequence Analysis for Microbial Identification Single assay 20