202003226-C EMGS.pdf

Client Job: 1-9106791 PO Number: 22414

JobNumber: 202003226

Routing Number: -

# Samples: 9

SPCT2

MYC Spore Count of Spore Trap Sample, 2 Mags

Client:

Modified ASTM D7391-17 Fungal Spore Count on Spore Trap Sample, Stratified

Method and Analysis Information:

Each incoming spore trap sample is disassembled, if needed, and the sample surface assembled onto a new 1”x3” glass slide marked with the sample’s unique lab identification number. Approximately 25 ul of stain is placed on the sample, and a glass cover slip is placed on top of the stain to complete the sample mounting preparation. The entire sample is then scanned at 500-630x magnification (depending on microscope used) on an optical light microscope. This initial scan is used to count relatively large and easily identifiable spores. Clumps and strings of small spores are also counted during this scan, using higher magnification if necessary for identification. Generally, single or scattered small spores are not counted at this magnification, although they might be in certain lightly loaded samples. Not all small spores (e.g., Cladosporium, Penicillium/Aspergillus, basidiospores, ascospores) are visible or identifiable at that magnification, however, so a second scan of a portion of the sample (nominally 30 passes across the debris zone) is made at 1000x magnification using oil immersion optics. This magnification allows the identification of single small spores that may not have been evident at the lower magnification. The resulting counts from the two scans are combined, for each genus or category, into

1) spores/sample (which is actual if the spore or category has been counted for the entire slide, or calculated if the spore or category has been counted at 1000x), 2) spores/m3 and 3) percentage of spores vs total spores. Each calculated value in the "Calc. Cts/smp", "Calc. Cnts/m3" and

"Calc. %"each column is reported to 2 significant digits, which can result in the total of the column numbers being different than the calculated total due to rounding; however, each number is correct to the number of significant digits presented. Percents are reported to the nearest whole percent.

The counting rules were as follows: Mold spores within the field of view are counted and, if possible, unambiguously categorized according to genera.

Those spores that cannot be unambiguously identified are categorized as miscellaneous/unidentified. Results are reported to the analytical sensitivity (hypothetical observation of one count during the analysis). Spore types having zero counts are reported as < the analytical sensitivity. The coefficient of variation (CV) for this analysis is estimated to be 0.2 for raw counts >50, and as high as 1.0 for low counts. The spores counted in this analysis may or may not be able to produce a living colony of mold. Therefore, this analysis is sometimes referred to as a "non-viable" count or method. This distinguishes it from a count of colonies on agar, referred to as a "viable" count. "Max. % coverage" equals the maximum area of the spore trap covered by particulate observed during the passes - it is an index of sample loading. Maximum coverages of >50% could have significant negative bias, as debris stacks on top of spores. Percent coverages >75% will have significant negative bias. Maximum coverages >95% are not countable.

The quality assurance program for mold spore counts includes the following. One lab blank per day provides a long-term track record of lab cleanliness. Each analyst holds at least a bachelor's degree in science and has attended the 5 day intensive Indoor Air Quality: Microscopy of Dust, Spores & Pollen training course or equivalent. Each day, the scope alignment is checked and documented. All analysts participate in interlab round robins. Ten percent of samples are re-counted by either the same analyst or another analyst to determine analyst precision and bias.All quality checks performed for these samples were in control except as detailed in the "Analytical Notes" below.

The results are shown following this cover sheet. Each lab analysis refers only to the sample tested, and may not, due to the sampling process, be representative of the air sampled. This report may not be reproduced except in full and with the approval of Fiberquant Analytical Services.

Method: Stratified SOP SPCT2Rec: 3/26/2020

Job Analysis Notes:

Client samples number 22414-01,02,03,04 and 05 were very heavily loaded with debris. As such, the actual spore count might be higher than reported.

Report Date: 3/27/2020 Date Analyzed: 3/27/2020

Fiberquant Internal SOP:

Some results may have been calculated using client supplied data, such as volume or area sampled, for which Fiberquant assumes no liability for accuracy.

FACILITY SERVICES MANAGEMENT

CLARKSTON RD BLDG 30033

FT HUACHUCA, AZ

Office Phone: (520) 533-5906

FAX:

85613-0000

5025 S. 33rd Street Phoenix, Arizona 85040-2816 Phone: 602-276-6139 1-800-743-2687 FAX: 602-276-4558

Fiberquant, Inc. Page 1 of 3

Spore Type Ranking

Job #: 1-9106791

Sample Num. Location Sampled Count/M3

Mycelial Total Fungal Fungal Spore Identification

Job Number: 202003226

See Detail Report Page (Following)

720Area Air/Hallway @ Cage 4 37,00022414-01 32,000 Penicillium/Aspergillus-like

2,800 Stachybotrys/Memnoniella

1,100 basidiospores

890 Cladosporium

280 smuts/Myxomycetes

200 Chaetomium

33 Torula

33 Curvularia

17 miscellaneous/unidentifiable

1,200Area Air/Hallway @ Cage 3 38,00022414-02 34,000 Penicillium/Aspergillus-like

1,300 Stachybotrys/Memnoniella

970 Cladosporium

890 basidiospores

420 smuts/Myxomycetes

130 Chaetomium

120 Alternaria

81 ascospores

50 Drechslera/Helminthosporium/Bipolaris

570Area Air/Hallway @ Cage 2 33,00022414-03 30,000 Penicillium/Aspergillus-like

730 basidiospores

700 Stachybotrys/Memnoniella

570 smuts/Myxomycetes

490 Cladosporium

230 Chaetomium

67 Alternaria

50 Drechslera/Helminthosporium/Bipolaris

1,100Area Air/Inside Cage 3 41,00022414-04 38,000 Penicillium/Aspergillus-like

970 basidiospores

950 Stachybotrys/Memnoniella

470 smuts/Myxomycetes

320 Cladosporium

300 Chaetomium

120 Alternaria

81 ascospores

17 Drechslera/Helminthosporium/Bipolaris

5025 S. 33rd Street Phoenix, Arizona 85040-2816 Phone: 602-276-6139 1-800-743-2687 FAX: 602-276-4558

Fiberquant, Inc. Page 2 of 3

730Area Air/Basement Hallway Entry 33,00022414-05 30,000 Penicillium/Aspergillus-like

890 basidiospores

630 smuts/Myxomycetes

400 Cladosporium

240 ascospores

230 Stachybotrys/Memnoniella

230 Alternaria

33 miscellaneous/unidentifiable

17 Chaetomium

230Area Air/O/ Bldg, West side 1,20022414-06 400 basidiospores

320 Penicillium/Aspergillus-like

200 ascospores

100 Alternaria

83 smuts/Myxomycetes

83 Stachybotrys/Memnoniella

17 Spegazzinia

83Area Air/O/ Bldg, East side 97022414-07 490 Cladosporium

160 ascospores

160 Penicillium/Aspergillus-like

81 basidiospores

33 smuts/Myxomycetes

33 Alternaria

17 Chaetomium

< 81Field Blank < 8122414-08 -

< 81Media Blank < 8122414-09 -

5025 S. 33rd Street Phoenix, Arizona 85040-2816 Phone: 602-276-6139 1-800-743-2687 FAX: 602-276-4558

Fiberquant, Inc. Page 3 of 3

Job Number: 202003226Mold Spore Count on Zefon Air-O-Cell Filter

Client Job: 1-9106791

Samples Submitted by: BILLY BARNES 3/26/2020

Cassette Type: Zefon

FACILITY SERVICES MANAGEMENT

CLARKSTON RD BLDG 30033

FT HUACHUCA, AZ

Office Phone: (520) 533-5906

FAX:

85613-0000

Genus or Type of Spore

Calc.

Cnts/m3

Med Mag

Counts

37,000

Calc. %

2.4

32,000

1,100

< 81

< 81

2,800

< 81

< 81

< 81

< 81

< 81

< 81

< 81

43 720

GVAnalyst:

3/24/2020Sample Date :

22414-01Client Number:

60Volume (L):

20.6% Ctd @ 1000x:

Cladosporium

Penicillium/Aspergillus-like

Basidiospore

Alternaria

Drechslera/Helminthosporium/Bipolaris

Stachybotrys/Memnoniella

Ascospore

Miscellaneous or Unidentifiable

Total Fungal Spores

Torula

Spegazzinia

Mycelial Fragments

2020-03226- 1Lab Number:

4Microscope #:

Area Air/Hallway @ Cage 4Description:

12 200Chaetomium

0 < 81Pithomyces

17 280smuts/Myxomycetes

0 < 81Ulocladium

4debris rating

2 33Curvularia

0 < 81Pollen

1000x

Counts

86.4

3.1

0.0

0.0

0.0

7.6

0.5

0.1

0.0

0.8

0.0

0.1

0.0

0.0

0.0

0.0

0.0

0.0

0.0

100.0

Calc.

Cts/smp.

2,200

1,900 acceptableCondition:

Rep.Lim.

YesR:

Genus or Type of Spore

Calc.

Cnts/m3

Med Mag

Counts

38,000

Calc. %

2.5

34,000

1,300

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

72 1,200

GVAnalyst:

3/24/2020Sample Date :

22414-02Client Number:

60Volume (L):

20.6% Ctd @ 1000x:

Cladosporium

Penicillium/Aspergillus-like

Basidiospore

Alternaria

Drechslera/Helminthosporium/Bipolaris

Stachybotrys/Memnoniella

Ascospore

Miscellaneous or Unidentifiable

Total Fungal Spores

Torula

Spegazzinia

Mycelial Fragments

2020-03226- 2Lab Number:

4Microscope #:

Area Air/Hallway @ Cage 3Description:

8 130Chaetomium

0 < 81Pithomyces

25 420smuts/Myxomycetes

0 < 81Ulocladium

4debris rating

0 < 81Curvularia

17 280Pollen

1000x

Counts

87.0

2.3

0.2

0.3

0.1

3.4

0.3

0.0

0.0

1.1

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

100.0

Calc.

Cts/smp.

2,300

2,000 acceptableCondition:

Rep.Lim.

YesR:

Genus or Type of Spore

Calc.

Cnts/m3

Med Mag

Counts

33,000

Calc. %

1.5

30,000

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

34 570

GVAnalyst:

3/24/2020Sample Date :

22414-03Client Number:

60Volume (L):

20.6% Ctd @ 1000x:

Cladosporium

Penicillium/Aspergillus-like

Basidiospore

Alternaria

Drechslera/Helminthosporium/Bipolaris

Stachybotrys/Memnoniella

Ascospore

Miscellaneous or Unidentifiable

Total Fungal Spores

Torula

Spegazzinia

Mycelial Fragments

2020-03226- 3Lab Number:

4Microscope #:

Area Air/Hallway @ Cage 2Description:

14 230Chaetomium

0 < 81Pithomyces

34 570smuts/Myxomycetes

0 < 81Ulocladium

4debris rating

0 < 81Curvularia

8 130Pollen

1000x

Counts

90.0

2.2

0.0

0.2

0.2

2.1

0.7

0.0

0.0

1.7

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.1

100.0

Calc.

Cts/smp.

2,000

1,800 acceptableCondition:

Rep.Lim.

YesR:

5025 S. 33rd Street Phoenix, Arizona 85040-2816 Phone: 602-276-6139 1-800-743-2687 FAX: 602-276-4558

Fiberquant Analytical ServicesDetail Report Page (see also cover page) 1 of 4

Note: all calculated values (indicated by "calc.") are rounded to two significant digits, which may produce apparent differences between a tabulated total and a calculated total. Rep. Lim. = Reporting Limit.

Samples Submitted by: BILLY BARNES 3/26/2020

Cassette Type: Zefon

FACILITY SERVICES MANAGEMENT

CLARKSTON RD BLDG 30033

FT HUACHUCA, AZ

Office Phone: (520) 533-5906

FAX:

85613-0000

Genus or Type of Spore

Calc.

Cnts/m3

Med Mag

Counts

41,000

Calc. %

0.8

38,000

< 81

< 81

< 81

< 81

< 81

< 81

< 81

67 1,100

GVAnalyst:

3/24/2020Sample Date :

22414-04Client Number:

60Volume (L):

20.6% Ctd @ 1000x:

Cladosporium

Penicillium/Aspergillus-like

Basidiospore

Alternaria

Drechslera/Helminthosporium/Bipolaris

Stachybotrys/Memnoniella

Ascospore

Miscellaneous or Unidentifiable

Total Fungal Spores

Torula

Spegazzinia

Mycelial Fragments

2020-03226- 4Lab Number:

4Microscope #:

Area Air/Inside Cage 3Description:

18 300Chaetomium

0 < 81Pithomyces

28 470smuts/Myxomycetes

0 < 81Ulocladium

4debris rating

0 < 81Curvularia

2 33Pollen

1000x

Counts

92.0

2.3

0.2

0.3

0.0

2.3

0.7

0.0

0.0

1.1

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

100.0

Calc.

Cts/smp.

2,500

2,300 acceptableCondition:

Rep.Lim.

YesR:

Genus or Type of Spore

Calc.

Cnts/m3

Med Mag

Counts

33,000

Calc. %

1.2

30,000

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

44 730

GVAnalyst:

3/24/2020Sample Date :

22414-05Client Number:

60Volume (L):

20.6% Ctd @ 1000x:

Cladosporium

Penicillium/Aspergillus-like

Basidiospore

Alternaria

Drechslera/Helminthosporium/Bipolaris

Stachybotrys/Memnoniella

Ascospore

Miscellaneous or Unidentifiable

Total Fungal Spores

Torula

Spegazzinia

Mycelial Fragments

2020-03226- 5Lab Number:

4Microscope #:

Area Air/Basement Hallway EntryDescription:

1 17Chaetomium

0 < 81Pithomyces

38 630smuts/Myxomycetes

0 < 81Ulocladium

4debris rating

0 < 81Curvularia

33 550Pollen

1000x

Counts

90.0

2.7

0.8

0.7

0.0

0.7

0.1

0.0

0.0

1.9

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.1

100.0

Calc.

Cts/smp.

2,000

1,800 acceptableCondition:

Rep.Lim.

YesR:

Genus or Type of Spore

Calc.

Cnts/m3

Med Mag

Counts

< 81

1,200

Calc. %

0.0

< 81

< 81

< 81

< 81

< 81

< 81

< 81

14 230

GVAnalyst:

3/24/2020Sample Date :

22414-06Client Number:

60Volume (L):

20.6% Ctd @ 1000x:

Cladosporium

Penicillium/Aspergillus-like

Basidiospore

Alternaria

Drechslera/Helminthosporium/Bipolaris

Stachybotrys/Memnoniella

Ascospore

Miscellaneous or Unidentifiable

Total Fungal Spores

Torula

Spegazzinia

Mycelial Fragments

2020-03226- 6Lab Number:

4Microscope #:

Area Air/O/ Bldg, West sideDescription:

0 < 81Chaetomium

0 < 81Pithomyces

5 83smuts/Myxomycetes

0 < 81Ulocladium

2debris rating

0 < 81Curvularia

32 530Pollen

1000x

Counts

25.7

32.4

16.2

8.1

0.0

6.8

0.0

0.0

0.0

6.8

0.0

0.0

1.4

0.0

0.0

0.0

0.0

0.0

1.4

100.0

Calc.

Cts/smp.

acceptableCondition:

Rep.Lim.

5025 S. 33rd Street Phoenix, Arizona 85040-2816 Phone: 602-276-6139 1-800-743-2687 FAX: 602-276-4558

Fiberquant Analytical ServicesDetail Report Page (see also cover page) 2 of 4

Note: all calculated values (indicated by "calc.") are rounded to two significant digits, which may produce apparent differences between a tabulated total and a calculated total. Rep. Lim. = Reporting Limit.

Samples Submitted by: BILLY BARNES 3/26/2020

Cassette Type: Zefon

FACILITY SERVICES MANAGEMENT

CLARKSTON RD BLDG 30033

FT HUACHUCA, AZ

Office Phone: (520) 533-5906

FAX:

85613-0000

Genus or Type of Spore

Calc.

Cnts/m3

Med Mag

Counts

Calc. %

50.0

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

5 83

GVAnalyst:

3/24/2020Sample Date :

22414-07Client Number:

60Volume (L):

20.6% Ctd @ 1000x:

Cladosporium

Penicillium/Aspergillus-like

Basidiospore

Alternaria

Drechslera/Helminthosporium/Bipolaris

Stachybotrys/Memnoniella

Ascospore

Miscellaneous or Unidentifiable

Total Fungal Spores

Torula

Spegazzinia

Mycelial Fragments

2020-03226- 7Lab Number:

4Microscope #:

Area Air/O/ Bldg, East sideDescription:

1 17Chaetomium

0 < 81Pithomyces

2 33smuts/Myxomycetes

0 < 81Ulocladium

2debris rating

0 < 81Curvularia

19 320Pollen

1000x

Counts

17.2

8.6

17.2

3.4

0.0

0.0

1.7

0.0

0.0

3.4

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

100.0

Calc.

Cts/smp.

acceptableCondition:

Rep.Lim.

YesR:

Genus or Type of Spore

Calc.

Cnts/m3

Med Mag

Counts

< 81

< 81

Calc. %

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

0 < 81

GVAnalyst:

3/24/2020Sample Date :

22414-08Client Number:

60Volume (L):

20.6% Ctd @ 1000x:

Cladosporium

Penicillium/Aspergillus-like

Basidiospore

Alternaria

Drechslera/Helminthosporium/Bipolaris

Stachybotrys/Memnoniella

Ascospore

Miscellaneous or Unidentifiable

Total Fungal Spores

Torula

Spegazzinia

Mycelial Fragments

2020-03226- 8Lab Number:

4Microscope #:

Field BlankDescription:

0 < 81Chaetomium

0 < 81Pithomyces

0 < 81smuts/Myxomycetes

0 < 81Ulocladium

1debris rating

0 < 81Curvularia

0 < 81Pollen

1000x

Counts

Calc.

Cts/smp.

acceptableCondition:

Rep.Lim.

YesR:

Genus or Type of Spore

Calc.

Cnts/m3

Med Mag

Counts

< 81

< 81

Calc. %

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

< 81

0 < 81

GVAnalyst:

3/24/2020Sample Date :

22414-09Client Number:

60Volume (L):

20.6% Ctd @ 1000x:

Cladosporium

Penicillium/Aspergillus-like

Basidiospore

Alternaria

Drechslera/Helminthosporium/Bipolaris

Stachybotrys/Memnoniella

Ascospore

Miscellaneous or Unidentifiable

Total Fungal Spores

Torula

Spegazzinia

Mycelial Fragments

2020-03226- 9Lab Number:

4Microscope #:

Media BlankDescription:

0 < 81Chaetomium

0 < 81Pithomyces

0 < 81smuts/Myxomycetes

0 < 81Ulocladium

1debris rating

0 < 81Curvularia

0 < 81Pollen

1000x

Counts

Calc.

Cts/smp.

acceptableCondition:

Rep.Lim.

5025 S. 33rd Street Phoenix, Arizona 85040-2816 Phone: 602-276-6139 1-800-743-2687 FAX: 602-276-4558

Fiberquant Analytical ServicesDetail Report Page (see also cover page) 3 of 4

Note: all calculated values (indicated by "calc.") are rounded to two significant digits, which may produce apparent differences between a tabulated total and a calculated total. Rep. Lim. = Reporting Limit.

Samples Submitted by: BILLY BARNES 3/26/2020

Cassette Type: Zefon

FACILITY SERVICES MANAGEMENT

CLARKSTON RD BLDG 30033

FT HUACHUCA, AZ

Office Phone: (520) 533-5906

FAX:

85613-0000

27-Mar-20Analyst: GALINA B. VOLKOVA

Larry S. Pierce, Approved Accreditation Signatory

Printed:

27-Mar-20Original Print Date:

5025 S. 33rd Street Phoenix, Arizona 85040-2816 Phone: 602-276-6139 1-800-743-2687 FAX: 602-276-4558

Fiberquant Analytical ServicesDetail Report Page (see also cover page) 4 of 4

Note: all calculated values (indicated by "calc.") are rounded to two significant digits, which may produce apparent differences between a tabulated total and a calculated total. Rep. Lim. = Reporting Limit.