IRR_Appendix_A_Deliverables.pdf

Appendix A - Deliverables

Quantity

464 boxes

745 kits

370 kits

118 boxes

45 kits

358 bottles

Appendix B

Specification Quantity per Vial

Live influenza viruses - human seasonal 1 6 virus @ 200 vials each 0.5 mL per vial

Live influenza viruses - animal origin 1 1 virus @ 200 vials each 0.5 mL per vial

Live influenza viruses - high path/select agent 1 1 virus @ 100 vials each 0.5 mL per vial

Live influenza viruses - sequencing grade 2 1 virus @ 100 vials each 0.5 mL per vial

BPL-inactivated seasonal human influenza viruses 3 6 virus @ 100 vials each 0.5 mL per vial

Genomic RNA from Influenza Virus 4 1 virus @ 200 vials each 0.1 mL per vial

Ferret antisera to seasonal human influenza viruses 5 1 virus @ 100 vials each 1 mL per vial

Ferret antisera to influenza candidate vaccine viruses 6 1 antisera @ 100 vials each 1 mL per vial

Influenza recombinant proteins 7 1 proteins @ 400 vials each 0.25 mL per vial

Cell lines for influenza virus propogation 8 2 cell lines @ 200 vials each 1 mL per vial

Hybridoma cell line cultivation 9 1 hybridoma @ 10 vials each 1 mL per vial

Monoclonal antibody purification 10 2 mAb @ 100 vials each 0.5 mL per vial

1 lot @ 200 kits each

200 additional vials of each component, available a la carte

BPL-inactivated seasonal human influenza viruses for the WHO Kit 11 4 virus @ 400 vials each 10 mL per vial

Ether-extracted seasonal human influenza viruses for the WHO Kit 11 2 virus @ 400 vials each 5 mL per vial

Bromelain-treated seasonal human influenza viruses for WHO Kit antisera 11 3 virus @ 3 vials each 100 ug per vial

Monoclonal antibodies (crossreactive pools) for the WHO IFA Kit 12 2 mAb @ 400 vials each 0.5 mL per vial

Monoclonal antibodies (seasonal strains) for the WHO IFA Kit 12 2 mAb @ 400 vials each 0.5 mL per vial

Influenza A/B Typing 13 1 lot @ 550 kits each

Influenza A Subtyping 13 1 lot @ 550 kits each

Influenza B Genotyping 13 1 lot @ 550 kits each

Avian Influenza Subtyping 13 1 lot @ 550 kits each

Manufacturer Catalog Number

52906

95059-200

4387391

3335402001

FTD-2P-64

P1193

Qiagen

Quanta Biosciences

Applied Biosystems

Roche

Fast-track Diagnostics

PromegaNuclease-free water (50 mL, 2/PK)

Qiagen QIAamp Viral RNA Mini Kit

Influenza Reference and Surveillance Reagents

Part II: Influenza Reagents

Production Tasks

Part I: NCIRD-wide Reagents

Influenza Reagent Kit for Identification of Influenza Isolates ("WHO Kit")

Influenza Immunofluorescence Reagent Kit for Identification of Influenza Isolates

("WHO IFA Kit")

Influenza Real-time RT-PCR Kits (In Vitro Diagnostic)

Influenza Real-time RT-PCR Kits (Research Use Only) qScript One-Step Quantitative RT-PCR Kit, Low ROX

AgPath-ID One-Step RT-PCR Kit

Protector Rnase Inhibitor

FTD® Respiratory pathogens Kit

NCIRD-wide Ancillary Reagents

Lot Size

Influenza A/B Typing 14 1 lot @ 550 kits each

Influenza A Subtyping 14 1 lot @ 550 kits each

Influenza B Genotyping 14 1 lot @ 550 kits each

Avian Influenza Subtyping 14 1 lot @ 550 kits each

1 lot @ 25 kits each

25 additional vials of each component, available a la carte

Influenza A Typing 15 1 primer pool @ 50 vials each

Influenza B Typing 15 1 primer pool @ 50 vials each

16 4 lots @ 100 sets each

17 4 lot @ 200 vials each

Non-Influenza Respiratory Panel, Organisms 18 1 virus @ 100 panels each

Influenza Virus Isolation and Identification Panel 18 2 virus @ 100 panels each

Influenza Virus Isolation and Identification Panel, Supplemental Challenge

Viruses

18 1 virus @ 100 panels each

Influenza Antigenicity Panel 18 1 virus @ 100 panels each

Influenza Next Generation Performance Evaluation Panel 18 1 virus @ 100 panels each

Influenza Next Generation Sequencing Limits of Detection Panel 18 1 virus @ 100 panels each

Influenza Sequencing Panel 18 1 virus @ 100 panels each

Influenza Molecular Diagnostic Performance Evaluation Panel 18 2 lot @ 120 panels each

Antiviral Resistance Panel 18 1 lot @ 100 panels each

Quantity

380 boxes

310 boxes

60 boxes

30 boxes

310 boxes

404 boxes

40 boxes

40 boxes

70 boxes

70 boxes

30 boxes

70 boxes

70 boxes

70 boxes

70 boxes

880 kits

70 kits bioMerieux bioMerieux bioMerieux

Invitrogen

Illumina

3038505001

6543588001

6374913001

61904

62724

19073

959034

280133

280130

280131

280132

11732-088

FC-131-1096

bioMerieux 200292

NucliSENS easyMag Buffer 1

NucliSENS easyMag Buffer 2

NucliSENS easyMag Buffer 3

SuperScript III Platinum One-Step Quantitative RT-PCR System without Rox

Nextera® XT DNA library Preparation Kit (96 Samples)

MagNA Pure Compact Nucleic Acid Isolation Kit 1

MagNA Pure LC Total Nucelic Acid Isolation Kit

Influenza Real-time RT-PCR Sets

Influenza Real-time RT-PCR Positive Controls

Influenza Reagent Panels

Influenza Multi-segment Reverse Transcription (MRT) PCR Kits

Influenza Ancillary Reagents

Qiagen EZ1 RNA Tissue Mini Kit

NucliSENS easyMag Magnetic Silica

NucliSENS easyMag Disposables

NucliSENS easyMag Lysis Buffer

NucliSENS easyMag Lysis Buffer

Manufacturer Catalog Number

MagNA Pure 96 DNA and Viral NA Small Volume Kit

MagNA Pure 96 External Lysis Buffer

Qiagen QIAamp DSP Viral RNA Mini Kit

Qiagen EZ1 DSP Virus Kit

Qiagen Buffer AVL bioMerieux

Roche

Roche

Roche

Roche

Qiagen

Qiagen

Qiagen

Qiagen bioMerieux

280135

280134 bioMerieux

3730964001

8 kits

10 boxes

10 boxes

10 boxes

10 boxes

10 boxes

10 boxes

10 boxes

25 boxes

180 boxes

35 boxes

90 boxes

90 boxes

65 boxes

65 boxes

85 boxes

20 boxes

20 boxes

375 boxes

55 boxes

Appendix B

Specification Quantity per Vial

Streptococcus RT-PCR Detection Kits 19 3 lots @ 25 kits each

Neisseria RT-PCR Detection Kits 19 3 lots @ 25 kits each

Haemophilus RT-PCR Detection Kits 19 3 lots @ 25 kits each

Bordetella RT-PCR Detection Kits 19 3 lots @ 25 kits each

Bacterial Pathogen Detection RT-PCR Kits 19 1 lots @ 25 kits each

Atypical Respiratory Bacterial Pathogen RT-PCR Detection Kits 20 1 lots @ 25 kits each

Streptococcus RT-PCR Typing Kits 21 3 lots @ 25 kits each

Neisseria RT-PCR Typing Kits 22 3 lots @ 25 kits each

Haemophilus RT-PCR Typing Kits 22 3 lots @ 25 kits each

Bacterial Pathogen Typing RT-PCR Kits 22 3 lots @ 25 kits each

Streptococcus Conventional PCR Kits 23 3 lots @ 25 kits each

Streptococcus RT-PCR Positive Controls 24 3 lots @ 25 vails each 1.0 mL per vial

17-5113-01

972804

979007

979006

979009

PyroMark Wash Buffer, 200mL

PyroMark Q24 Cartridge

PyroMark Q96 Cartridge

Receptor Destroying Enzyme II (RDE II), Influenza Virus

Production Tasks Lot Size

Streptavidin Sepharose High Performance

PyroMark Gold Q96 Reagents

PyroMark Denaturation Solution, 500mL

PyroMark Binding Buffer, 200mL

PyroMark Annealing Buffer, 250mL

NA-Fluor Influenza Neuraminidase Assay Kit

MS-102-2022

A63881

929004

FC-131-1002

FC-131-2001

979008

979202

979004

YCC340122

4457091

FC-131-2002

FC-131-2003

FC-131-2004

S16-002

12574-035

Applied Biosystems

Qiagen

Illumina

Illumina

Illumina

Qiagen

Invitrogen

Illumina

Beckman Coulter

Qiagen

Illumina

Illumina

Miseq Reagent kit v2 (300 cycle) 20 pack

Agencourt AmPure XP

QIAxcel DNA Screening Kit (2400)

ISA Nextera XT Index Kit 96 indexes

Nextera XT Index Kit V2, Set A (96 indexes, 384 samples)

Nextera XT Index Kit V2, Set B (96 indexes, 384 samples)

Nextera XT Index Kit V2, Set C (96 indexes, 384 samples)

Nextera XT Index Kit V2, Set D (96 indexes, 384 samples)

Custom QIAamp 96 DNA Kit with Treated Plates for QIAcube HT

SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity

Bacterial Pathogen Typing RT-PCR Kits

Bacterial Pathogen Conventional PCR Kits

Bacterial Pathogen RT-PCR Controls

GE Healthcare

Qiagen

Qiagen

Qiagen

Qiagen

Qiagen

Qiagen

Accurate Chemical

Part III: Bacterial Diseases Reagents

Bacterial Pathogen Detection RT-PCR Kits

Bordetella RT-PCR Positive Controls 25 3 lots @ 25 kits each 0.5 mL per vial

Bacterial Pathogens RT-PCR Positive Controls 26 3 lots @ 25 vails each 0.5 mL per vial

Neissera Reference Strains 27 15 lots @ 25 kits each 0.5 mL per vial

Haemophilus Reference Strains 27 3 lots @ 25 kits each 0.5 mL per vial

Streptococcus Reference Strains 27 1 lots @ 25 kits each 0.5 mL per vial

Quantity

250 boxes

100 boxes

50 boxes

100 kits

200 kits

50 kits

30 bottles

50 bottles

100 kits

50 boxes

50 boxes

50 boxes

Appendix B

Specification Quantity per Vial

Cell Lines for Virus Propagation 28 2 lot @ 200 vials each 1 ml per vial

Norovirus RT-PCR Kit 29 1 lot @ 20 kits each

Rotavirus Genotyping RT-PCR Kit 30 1 lot @ 100 kits each

Measles Real-time RT-PCR Kit 31 1 lot @ 150 kits each

Rubella Real-time RT-PCR Kit 31 1 lot @ 150 kits each

Poliovirus ITD RT-PCR ITD Kit 32 1 lot @ 725 kits each

Vaccine-derived Poliovirus RT-PCR Kit 33 1 lot @ 350 kits each

Enterovirus Quadruplex RT-PCR Kit 34 1 lot @ 100 kits each

Non-Influenza Respiratory Virus RT-PCR Panel 35 1 lot @ 50 kits each

MERS-CoV rRT-PCR Panel 36 1 lot @ 50 kits each

Non-Influenza Respiratory Virus RT-PCR Kits 37 1 lot @ 50 kits each

410152Agilent 96-well plate sealing film

Part IV: Viral Diseases Reagents

Production Tasks Lot Size

H4632-10G

BP2473-500

955134

410088

401425

95114-012

206143

51306

BNX-710-000

BNX-710-010

Agilent

Agilent

Agilent

Hemoglobin equine lyophilized powder

Tris-EDTA Buffer, pH 8.0, 500mL

EZ1 Virus Mini Kit v2.0

Agilent Mx3005P 96 well tube plates

Agilent Mx3005P optical strip caps

PerfeCTa® qPCR ToughMix®, ow ROX™

QIAGEN Multiplex PCR Kit (100)

QIAamp DNA Mini Kit (250 samples)

BinaxNOW Streptococcus pneumoniae Test Kit

BinaxNOW ICT Strep Pneumo Cntl Kt 10pk

Quanta Biosciences

Qiagen

Qiagen

Inverness Medical Innovations

Inverness Medical Innovations

Sigma

Fisher Scientific

Qiagen

95063-200

Bacterial Diseases Ancillary Reagents Manufacturer Catalog Number

Quanta Biosciences

Respiratory Virus Real-time RT-PCR Kits

Bacterial Pathogen Reference Strains

Reference and Surveillance Reagents

Gastroenteritis Real-time RT-PCR Kits

Measles and Rubella Real-time RT-PCR Kits

Enterovirus Real-time RT-PCR Kits

PerfeCTa® Multiplex qPCR SuperMix (200 x 50ul rxns)

Human Ribonuclease P rRT-PCR Kit 37 1 lot @ 50 kits each

Novel Coronavirus 2012 Real-Time RT-PCR Positive Control 38 1 lot @ 75 vials each 1 mL per vial

Non-Influenza Respiratory Virus Real-Time RT-PCR Positive Control 38 1 lot @ 75 vials each 1 mL per vial

Adenovirus Typing Real-Time RT-PCR Positive Control 38 1 lot @ 75 vials each 1 mL per vial

Norovirus Conventional PCR Kit 39 1 lot @ 20 kits each

Rotavirus Conventional RT-PCR Kit 40 1 lot @ 150 kits each

Measles Genotyping PCR Kit 41 1 lot @ 120 kits each

Rubella Genotyping PCR Kit 42 1 lot @ 120 kits each

Rubella Diagnostic PCR Kit 43 1 lot @ 50 kits each

Poliovirus Sequencing Kit 44 5 lot @ 50 kits each

ITD Polio Proficiency Panel 45 4 lot @ 40 kits each

Polio Sequencing Proficiency Panel 45 5 lot @ 80 kits each

Quantity

20 boxes

25 boxes

64 boxes

36 boxes

42 boxes

54 boxes

20 kits

10 packs

39 packs

108 vials

20 boxes

1450 bottles

2 bottles

54 bottles

54 bag

54 vials

54 vials

108 vials

50 boxes

10 boxes

2530-CD-050

A29151

15581044

12574026

CS32050

28706

4376486

4337455

B0300-1VL

15597-B1

Biotium

Promega

ThermoFisher

GE Life Sciences

Lonza 57028

16500500

41003

G1881

15628019

WB120311

ThermoFisher

Qiagen

ThermoFisher

ThermoFisher

ThermoFisher

ThermoFisher

Qiagen

ThermoFisher

ThermoFisher

Sigma

ATCC

R&D Systems

Beckman Coulter

ThermoFisher

ThermoFisher

Flash Gel 1.2% 10 pack 2 tier

Invitrogen Ultra-Pure Agarose 500g

GelRed nucleic acid gel stain 10,000x 0.5ml

Blue/Orange loading dye, 6X 3ml

100 bp DNA ladder 50ug

Whatman Indicating FTA micro cards

Betaine, 5M

Escherichia coli bacteriophage MS2

Recombinant Human CD155/PVR Protein

Agencourt CleanSEQ 8mL

10X TBE (Tris-borate EDTA) buffer 1L

Invitrogen SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase 100Rxn

Invitrogen Charge-Switch PCR Clean-up kit

QIAquick Gel Extraction Kit (250)

BigDye XTerminator® Purification Kit (100 preps)

BigDye® Terminator v3.1 Cycle Sequencing Kit

AmpliTaq Gold DNA Polymerase with Buffer II and MgCl2 (250 units)

Qiagen - OneStep RT-PCR kit (100)

Invitrogen SuperScript® III Platinum® One-Step qRT-PCR Kit 100Rxn

Invitrogen SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity DNA Polymerase

100Rxn

N8080241

210212

11732020

12574035

Viral Diseases Ancillary Reagents Manufacturer Catalog Number

Enterovirus Reagent Panels

Respiratory Virus RT-PCR Controls

Gastroenteritis Conventional RT-PCR Kits

Measles and Rubella Conventional RT-PCR Kits

Enterovirus Sequencing Kits

12 boxes

12 boxes

12 boxes

40 boxes

9 boxes

5 boxes

5 boxes

5 boxes

5 boxes

10 boxes

Life Technologies

Life Technologies

Life Technologies

Life Technologies

Life Technologies 4311971

4393708

4393927

4408256

4306737

AB MicroAmp Optical Adhesive 100 covers

3500 Cathode Buffer

AB MicroAmp Optical 96 well Rxn plate 20 plates

3500 Polymer (384)

3500 Anode Buffer

Measles Virus IgG Virion/Serion ESR 102 G

ProSpecT Rotavirus Microplate Assay Fisher Scientific 11993132

Captia Rubella IgM Trinity Biotech 2325360

Captia Rubella IgG Trinity Biotech 2325300

Measles Virus IgM Virion/Serion ESR 102 M