2_JA 2076528.docx

Justification for Other than Full and Open Competition at or Below the Simplified Acquisition Threshold Project Title: FlowJo Software Licenses Bridge I. Agency/office:

DHHS, NIH, NIAID, RTB, PCS

Address of the requesting office: Chief, Flow Cytometry Section, Research Technologies Branch, Bldg. 4, Room B1-38

II. Description of the project, and supplies or services, required to meet the agency’s needs A. Project background The Flow Cytometry Section provides flow cyometric analysis and sorting for all of the Intramural program, NIAID, NIH. Purchase of this software licenses will guarantee the optimal function and therefore reliable data of the flow cytometry analysis and sorting for our customers.

B. Project description The Flow Cytometry Section of the Research Technologies Branch, NIAID, NIH performs flow Cytometric analysis, for the DIR, NIAID and utilizes highly sophisticated instrumentation including these analyzers and lasers, and software programs. The FlowJo software permits organizing and managing complex cytometry experiments and produces detailed graphical reports. This software is ubiquitous in use in the scientific community and has historically been the standard use. This license has been renewed annually to maintain continuity of experimental data analysis and because of the high desirability and functionality of the software

III. Identification of the statutory authority permitting other than full and open competition ☒ 41 USC 253 (C) (1) and FAR Part 6.302-1: only one responsible source

IV. Demonstration that the proposed contractor (s) unique qualifications or the nature of the acquisition requires use of the authority cited.

A. Name and address of the proposed contractor (s):

FlowJo LLC 385 Williamson Way Ashland, OR 97520 B. Describe why only the requested supply/ service will meet the government’s needs The Flow Cytometry Section’s provides flow cytometric analysis and sorting for all of the Intramural program, NIAID, NIH. This software works with NIAID’s flow cytometers to analyze raw flow cytometric data. Renewal of the FlowJo cytometric software site licenses will guarantee the optimal function and therefore reliable data of the flow cytometry analysis and sorting for NIAID investigators. FlowJo’s ability to automate repetitive operations facilitates the production of statistics tables and graphical reports when experiment(s) involve many samples, parameters and/or operations.