2. Bill of Requirements-Specifications posting attach.docx
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- Attached to
- Stunner Platform – benchtop micro-volume and high throughput protein characterization system (Brand Name or Equal) Federal contract opportunity
- Solicitation number
- RFQ-NIAID-23-2172175
About this file
This document outlines specifications for a federal contract solicitation. The National Institute of Allergy and Infectious Diseases is seeking a benchtop micro-volume and high throughput protein characterization system. Crucial features for the system include UV/Vis LNP total RNA quant, dynamic light scattering, static light scattering, and the ability to analyze minimal sample volumes less than 5 microliters within dimensions not exceeding 37 cm width by 54 cm depth by 33 cm height. Bidders should be able to provide analysis capabilities including AAV and AdV capsid titer measurement.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| 2. FAR 52.212-5 June 2023 - posting attachment.docx | DOCX document |
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Required specifications for the benchtop micro-volume and high throughput protein characterization system
| Crucial must have features |
| Good to have but not absolutely necessary featuers |
| UV/Vis |
| LNP total RNA quant |
| Dynamic light scattering (DLS) |
| AAV & AdV capsid titer |
Static light scattering (SLS)
Minimal amount of sample (< 5 uL)
Max dimensions ‐ 37 cm W x 54 cm D x 33 cm H; <35 kg
UV/Vis specifications Light source ‐ Xenon flash lamp Detectors ‐ UV/Vis polychromatic spectrophotometer Wavelength ‐ range230‐750 nm Wavelength accuracy ‐ ≤400nm: ±1nm; ≥400nm: ±2 nm Spectral resolution ‐ Better than 2 nm (toluene in hexane) Absorbance precision (1 cm quartz cuvette) <1 OD: ±0.005 OD; st dev 1‐2 OD: ±0.5% CV Absorbance accuracy (1 cm quartz cuvette) <1 OD: ±0.01 OD; 1‐2 OD: ±1%
DLS specifications Light source ‐ 660 nm laser diode Detection ‐ Avalanche photodiode module Size accuracy ‐ ±2% Minimum sample concentration ‐ 0.1 mg/mL lysozyme Hydrodynamic diameter range ‐ 0.3–1000nm Samples per plate 96 (12 x 8 microplate format) Measurement time for full plate ‐ <20 minutes for UV/Vis only; <90 min for UV/Vis and DLS Measurement range: OD 10 mm ‐ 0.03–275 OD ng/μL dsDNA ‐ 1.5–13750 ng/μL mg/mL ave protein mix ‐ 10 mm 0.03–275 mg/mL Absorbance precision (10 mm pathlength) <1 OD: ±0.01 OD; st dev 1‐200 OD: ±1% CV Absorbance accuracy (10 mm pathlength)<1
OD: ±0.02 OD; 1‐200 OD: ±2%
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