Class_III_Specs.docx

Specifications for a Class III Biosafety Cabinet The BSC must have the following Specifications:

Shall have a three glove, 60” working chamber with an attached one glove 24” pass‐through chamber.

Fully assembled, overall dimensions are not to exceed 86”tall x 85”wide x 34”deep. All components must fit through a 34”wide x 80”high doorway.

The 24” pass‐through chamber shall be on the Left side of the working chamber and be equipped with a forward opening hinged loading door.

The working chamber must have a hinged gull wing door that can be opened to allow loading of equipment and instruments.

The view screens must be made of 3/8” mirror quality scratch resistant safety glass and slanted at approximately 100 to enhance worker comfort.

The glove ports must be oval and built into the view screen. Gloves must be securely attached to the ports in a manner that prevents the gloves from accidentally coming off during use. Gloves must be able to be replaced without breaking containment.

Glove and sleeves shall be one piece and made of Hypalon.

All gasket material shall be made of Silicone.

All door and window seals will be double knife edge construction.

All metal components must be made of corrosion resisting steel. At a minimum, 11 gauge Grade 316 stainless steel for internal components and 304 stainless steel for external components.

Must allow for the safe and convenient changing of loaded exhaust HEPA filters.

Both the primary work chamber and the pass-through chamber incorporate vertical, top to bottom, unidirectional air flow.

Digital differential pressure gauge display with audible and visual alarms readily observable to the operator.

Electrical requirements:

115V 20 amp single phase, 60 cycle (USA Standard).

Two independently controlled/switched GFCI outlets are to be provided in the primary working chamber. Outlets are to be equipped with weather‐proof covers.

External fluorescent light assembly that provides at least 75‐100 foot candles of illumination to the work surface. Bulb/Tubes replacements must be serviceable from outside the contaminated zone. Both the pass‐through and the working chambers will be illuminated.

Factory/Manufacturer Testing:

Must meet all the requirements of a Class III Biological Safety Cabinet as defined by the Federal Register and CDC/NIH publication “Biosafety in Microbiological and Biomedical Laboratories” 5th Edition.

The manufacturer shall perform the at least the following tests and shall supply documentation verifying the results.

· Filter Leak Testing: To insure HEPA filter integrity and efficiency

· Electrical Testing

· Factory Helium Test: To validate the cabinet seal integrity of welds, gaskets, gloves, gloveattachment and any other penetrations pertaining to Class III Biological Safety Cabinets. Leaks shall not be more than 5 x 10‐7 cc/ s with an internal concentration of 5% Helium to air at 3” water column positive pressure.

· Cabinet Pressure Test: To verify continuous negative pressure of 0.5” wc