Specifications_for_Real-Time_PCR.docx

Specifications for 7500 Fast Real-Time PCR System

· FAST real-time PCR system

· 5 color detection system (FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5)

· Multiplexing technology up to 5 targets per well

· Results in 30 minutes

· Software that allows multiplexing, quick start, and quick export

· Expanded melt curve options

· Ability to use TaqMan Fast Universal PCR Master Mix

· 9 logs of linear dynamic range

· Detection of 1 copy of template in a 20 ul reaction for a single reporter TaqMan assay with a 99.7% confidence

· 96-well block

· CCD camera with halogen lamp excitation, 5-excitiation and 5-emmision filters

· Reaction volumes of 5-30 ul

· A peak block heating rate of 5.5o C

· A temperature range of 4-100o C

· Temperature accuracy of +/- 0.25o C

· Temperature uniformity of +/- 0.50o C

· Dimensions of 34 cm. X 45 cm. X 49 cm. and 34 kg in weight