Specifications.doc
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- Attached to
- Thermal Cycler & Meter Cable With Installation Federal contract opportunity
- Solicitation number
- 12305B24Q0037-II
About this file
This document is a Specifications sheet for an Automated Thermal Cycler to be integrated with the Beckman Coulter Biomek i7 and pre-existing library preparation program. The key requirements include:
Temperature range of 4C to 105C, temperature accuracy of +/- 0.25C, max block ramp rate of 3.5C/sec, max sample ramp rate of 1.8C/sec, 96-well block format compatible with full and half-skirted plates, PCR volume range of 10-100uL for full-skirted plates and 20-100uL for half-skirted plates, and temperature uniformity of 0.5C. The thermal cycler must be installed on the Beckman Coulter Biomek i7 deck by a certified technician, who must also update the KAPA HyperPlus Kit method to integrate the thermal cycler and test the integration.
The related federal contract opportunity is Solicitation Number 12305B24Q0037-II for "Thermal Cycler & Meter Cable With Installation". This is a combined synopsis/solicitation for commercial items/services with a Lowest Priced Technically Acceptable (LPTA) evaluation. Quotes are due by 4/16/2024 at 2:00 PM PST and must include a firm fixed price quote, place of manufacture, SAM.gov UEI#, and "equal to brand name" details if applicable. The place of delivery and acceptance is 10th & Constitution Ave, NW, MRC-0168, Washington DC 20660.
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| File | Type | Posted |
|---|---|---|
| Contract Terms and Conditions with Provisions.docx | DOCX document |
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SPECIFICATIONS
1.0 Specifications Description: Automated thermal cycler to be integrated with the Beckman Coulter Biomek i7 and pre-existing library preparation program.
Automated Thermal Cycler WITH Installation Quantity: 1 system
Unit Type: Each
Minimum requirements:
· Temperature range: 4°C to 105°C
· Temperature accuracy: +/- 0.25°C (35°C to 99.9°C)
· Max block ramp rate: 3.5°C/sec
· Max sample ramp rate: 1.8°C/sec
· Block format: 96-well, 0.2mL block, compatible with full and half-skirted plates
· PCR volume range: 10-100uL for full-skirted plates, 20-100uL for half-skirted plates
· Temperature uniformity: (0.50°C (20 sec after reaching 95°C)
· Temperature calibration: Calibrated to standards traceable to the National Institute of Standards and Technology (NIST)
· Software features: Application programming interfaces (APIs) available for integration with robotics systems
· Include a 3 meter cable Must:
· Automated thermal cycler must be installed on the Beckman Coulter Biomek i7 deck by a certified technician.
· A certified technician must update the current KAPA HyperPlus Kit (KK8514, half reactions) method to integrate the automated thermal cycler into the method program.
· A certified technician must test the integration of the automated thermal cycler and modified KAPA HyperPlus Kit (KK8514, half reactions) method from start to finish to ensure it works properly.
2.0
Place of Delivery
Christopher L. Owen USDA-ARS, c/o Smithsonian Institution, National Museum of Natural History, 10th and Constitution Avenue, NW, Washington, DC 20013-7012 3.0
Schedule
| Item |
| QTY |
| Unit |
| Place of Delivery |
| Delivery Date |
| Automated thermal cycler WITH installation |
| 1 |
| Each |
| See Section 2.0 |
| 60 Days After Award |
4.0
Delivery Point of Contact
Christopher L. Owen, christopher.owen@usda.gov, (202) 633-4560 5.0
Additional Requirements
Standard industry warranty must be included.
File details come from the government source that posted it. Updated .