512Specifications_for_Cryo_SEM_2019.docx
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- Cryo Scanning Electron Microscope Federal contract opportunity
- Solicitation number
- 12305B-19-CS-BARCSEM
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Specifications
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| 312asCI__(FAC_2019-4_082119).docx | DOCX document |
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Specifications for Cryo-Scanning Electron Microscope
Electron Microscope
| • | Three types of electron detectors capable of obtaining images at both low and high vacuum settings. 1. Five back scatter detectors (a 4-quadrant type and an annular type) especially for use at low vacuum settings. Capable of obtaining a “live” 3-dimensional (3D) image within two (2) minutes of initial scanning; 2. Three secondary electron detectors, one low, one mid-level and the other high that can be used individually or simultaneously for obtaining high resolution images. 3. An Ultra Variable Pressure Detector (UVD) unique in its ability to image in both high and low vacuum modes at a very high resolution but in cathodo-luminescence visible imaging which is essential for biological samples. |
| • | Capable of a large variable vacuum range (10-300Pa) (low vacuum) and high vacuum (7 x 10-4 Pa) with a range of 1 to 30kV which can be easily and quickly switched between low and high vacuum modes via software without the physical insertion or removal of adaptor biasing lens. |
| • | Fully integrated system with Schottky emission electron gun which provides a stable, high emission electron source for high magnification and resolution imaging. |
| • | Magnification of 20x – 2,000,000x with a resolution of 0.9nm at 1kV and 0.8nm at 15kV. The lower magnification (as low as 20X) in variable pressure mode is required to observe entire organisms prior to higher magnification imaging. |
| • | Utilizes a “heated” moveable objective aperture to improve the length of time the operator can use the SEM. |
| • | Allows for high definition image capture of up to 40,000 x 40,000 pixels to help preserve the fine details often seen from surface information. |
| • | Standard Size Adjustable Height Computer Table (45" W x 27" D x 22-48" H) with 2 monitor arms for two 24 inch monitors. |
Cryo-Prep System
| • | A gas cooled cryo-preparation system mounted onto the column of a SEM for observing high water content biological samples in pristine form without shrinkage or deformation due to vacuum pressure or high beam strength from the SEM. |
| • | The Prepdek workstation for preparing biological specimens for freezing, including plunge freezing and freeze fracturing. |
| • | Liquid nitrogen gas cooled preparation chamber for transferring biological samples from room temperature under atmospheric pressure to -130oC within a high vacuum SEM; decontamination of frosted samples (freeze etching); coating with platinum using a sputter coater for maximum contrast; and transfer into the cryo-stage inside the SEM. |
| • | Liquid nitrogen gas cooled cryo-stage inside the SEM with cold trap to prevent contamination. |
| • | Temperature control for the system is through the use of a recipe driven intuitive PC programmed touch-screen panel with CCD camera capabilities for observation and collection of data of the entire process. |
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