Specifications_for_Next_Generation_RNA_sequencing.docx

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Next Generation RNA Sequencing Federal contract opportunity
Solicitation number
12020219S0006
Issued by
Department of Agriculture Agricultural Research Service Field Research Implementation and Information Delivery Pacific West Area

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Specifications for Next Generation RNA sequencing

USDA-ARS is evaluating transcriptional regulation differences in strains of trout exposed bacterial and viral pathogens. For this project we have 720 RNA samples for analysis. USDA-ARS will supply RNA that meet Awardee quantity and quality specifications.

We need a service molecular laboratory that can perform the following:

1. Quality control of samples.

1. Library preparation of supplied RNA, strand specific library preparation

1. Quality control and quantitation of libraries

1. Next Generation Illumina Library sequencing 2 x 150 paired ends read with an average of 25 million reads per sample

1. Deliver the final analysis to include raw data files and pertinent sequence data to the USDA-ARS Lab in Hagerman, ID.

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