1-PD.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The purpose of this procurement is the acquisition of these 14 different MOUSE ON-TARGETplus siRNA libraries for the Functional Genomic Lab. We need Horizon to provide us with this array of siRNA reagents specifically targeting mouse genes in RNAi screening.

2. Background Information and Objective: The National Institutes of Health has established an RNAi facility to provide intramural researchers with the equipment and expertise necessary to conduct genome-wide RNAi screens in mammalian cells. This facility works as a collaborative resource for doing RNAi assay development, screening, and initial follow-up. Our facility works with investigators to develop and optimize their assays for screening, conduct the screens and related informatics analyses, then performs follow-up screens on promising leads. Further work is done with the PI to do the downstream biology required to get the quality publications and the kind of groundbreaking results needed. This request is required for this facility to develop a flexible functional genomics platform that offers complementary and orthogonal RNAi screens to NIH intramural investigators. Horizon provides several predesigned RNAi screening libraries, which allows us to conduct RNAi screenings effectively. This item is needed to facilitate our RNAi screening program. This program offers intramural researchers the equipment and expertise to conduct large-scale functional genomics screens.

3. Generic Name of Product: Mouse ON-TARGETplus siRNA Library, 0.1 nmole library

4. Purchase Description: Qty 14 - siRNA Mouse ON-TARGETplus siRNA libraries

1. Serine Proteases- SMARTpool, 0.1 nmole library

2. Transcription Factors- SMARTpool, 0.1 nmole library

3. Tyrosine Kinases- Set of 4, 0.1 nmole library

4. Cell Cycle- Set of 4, 0.1 nmole library,

5. Nuclear Receptors- Set of 4, 0.1 nmole library

6. Protein Kinases- Set of 4, 0.1 nmole library

7. GPCR- Set of 4, 0.1 nmole library

8. Phosphatases- Set of 4, 0.1 nmole library

9. Ion Channels- Set of 4, 0.1 nmole library

10. Cytokine Receptors-Set of 4, 0.1 nmole library

11. Deubiquitinating Enzymes-Set of 4, 0.1 nmole library

12. Proteases-Set of 4, 0.1 nmole library

13. Membrane Trafficking- Set of 4, 0.1 nmole library

14. Epigenetics-Set of 4, 0.1 nmole library

5. Salient characteristics:

In RNAi screens, small-interfering RNA (siRNA) are small double-stranded RNA molecules that target complementary mRNA and silence this target gene. Alongside siRNA are endoribonucleases, called RNA-induced silencing complex (RISCs), and Dicer enzymes, which help prepare siRNAs to bind to their target sequence. So, for RNAi screening, it is useful to have

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs an array of siRNAs that can target several genes of interest, which in our case would be mouse genes. Horizon will produce an siRNA library that includes siRNA reagents targeting mouse genes.

In these libraries, Horizon includes a dual- strand modification on the siRNAs and in the provided plate, includes siRNA in set of 4. These two features are imperative in gaining on-target and robust screening results. We will receive 14 different siRNA libraries containing siRNAs that target a specific intracellular process or protein.

6. Quantity: 14 Mouse ON-TARGETplus siRNA libraries

7. Delivery Date:

30 days ARO