The National Institute of General Medical Sciences (NIGMS) awarded a 5-year, $933,656 Project Grant (CFDA 93.859 Biomedical Research and Research Training) to the University of Chicago to uncover the mechanisms of cell-type-specific pre-mRNA splicing. The overarching goal is to develop new tools and datasets to understand how cis-regulatory elements and RNA-binding proteins regulate splice site selection in different cell types, with a focus on the mouse brain.
The research approach involves: 1) using single-cell long-read sequencing to identify cis-regulatory elements and coordinated splicing patterns, 2) studying redundant RNA-binding protein functions through multiplexed genome editing, and 3) investigating protein-RNA interactions at the cell-type level using dual RNA-deaminase editing and sequencing. Successful completion of this project is expected to generate new capabilities for understanding the mechanisms driving cell-type-specific pre-mRNA processing.
Generated 1/28/25, 9:54 AM