This federal Project Grant award from the National Institutes of Health (NIH) Office of Research Infrastructure Programs (CFDA 93.351) provides $779,657 to the University of Colorado-Denver to establish and expand community-accessible resources for site-directed transgenesis in zebrafish. The project aims to generate next-generation landing site strains for reporter and enhancer testing applications, as well as streamline mapping of established transgenes to identify additional safe-harbor sites for diverse biomedical research applications. The award period runs from July 15, 2025 to June 30, 2029. This work builds on the grantee's pioneering efforts on PhiC31 integrase transgenesis in zebrafish, with the goal of advancing transgenic capabilities and reducing the resources required to generate transgenic zebrafish models across multiple NIH research interests.