This Project Grant award from the National Institute of Allergy and Infectious Diseases (NIAID), under the Allergy and Infectious Diseases Research program (CFDA 93.855), provides $476,453 to Boston University Medical Campus to establish intein-based tools to study Ebola virus (EBOV) replication. The goals are to harness intein chemistry to develop technologies for studying this deadly, reemerging pathogen, including creating a self-removing fluorescent reporter system and an "off-switch" to inactivate newly translated viral proteins. The award will fund research to systematically evaluate intein sequence and structural features, develop optimized intein-based reporters, and establish an intein-based system to reversibly inactivate EBOV proteins. The project will also build upon prior discoveries, including identifying intein variants with enhanced catalytic properties through virus passaging. A $134,500 sub-award to Murray State University will support work examining intein splicing efficiency and factors governing splicing, to inform the design of intein-based tools for EBOV research. The award period runs from July 1, 2024 to May 31, 2026.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $25.1k | 9/11/24 | ||
| Not listed | $225.7k | 6/27/24 | ||
| Not listed | $225.7k | 6/27/24 |
Grant Number | Description | Subgrantee | Prime Award | Dollars Obligated (Click to sort descending) | Updated At (Click to sort ascending) |
|---|---|---|---|---|---|
4500005134S | Murray State University | Project Grant R21AI178560 | $29.0k | 11/11/24 |