The University of South Florida (USF) received a $200,323 Project Grant award from the National Institute on Aging (CFDA 93.866 - Aging Research) to investigate the aggregation of amyloid beta (Aβ) peptides during metabolic stress and spreading depolarization in the brain. The project will use multiscale modeling, high-resolution microscopy, antibody staining, and measurement of paralysis phenotype in C. elegans to determine the role of physiochemical changes due to metabolic stress and spreading depolarization in the onset and progression of Aβ40 and Aβ42 aggregation. This research aims to better understand the conditions leading to Aβ aggregation at spatiotemporal scales not feasible through experiments alone, with broader applications in computational neuroscience. The award has a project period from September 1, 2024 to June 30, 2026.