This $401,490 federal Project Grant award from the National Science Foundation's Engineering program (CFDA 47.041) is funding the development of a modular toolset for imaging cellular metabolites using fluorogenic RNA riboswitches. The key objectives are to: (1) engineer allosteric fluorogenic RNA "Broccoli"-based metabolite sensors with various riboswitch classes, (2) develop an in silico platform to improve probe design and sensitivity, and (3) obtain quantitative, multi-colored, and time-resolved imaging profiles of diverse metabolites in individual bacterial and mammalian cells. This research aims to enhance the real-time detection of cellular metabolites like sugars and fatty acids, which is crucial for understanding human health, nutrition, plant science, food safety, and environmental studies. The award will also support interdisciplinary scientist training and middle school student engagement through workshops and lab tours. The University of Massachusetts, Amherst is the prime awardee on this 3-year project running from May 2025 to April 2028.
Mod # | Description | Reason For Modification | Federal Obligation (Click to sort descending) | Date (Click to sort ascending) |
|---|---|---|---|---|
| Not listed | $401.5k | 6/10/25 |